Process optimization for enhanced production of cellulases form locally isolated fungal strain by submerged fermentation

Roheena Abdullah; Ammara Akhtar; Kinza Nisar; Afshan  Kaleem; Mehwish Iqtedar; Tehreema Iftikhar; Faiza Saleem; Farheen  Aslam

doi:10.14393/BJ-v37n0a2021-53815

Authors

Roheena Abdullah Lahore College for Women University https://orcid.org/0000-0003-3932-6297
Ammara Akhtar Lahore College for Women University https://orcid.org/0000-0002-3497-5004
Kinza Nisar Lahore College for Women University https://orcid.org/0000-0003-0424-4320
Afshan Kaleem Lahore College for Women University https://orcid.org/0000-0002-3972-0051
Mehwish Iqtedar Lahore College for Women University https://orcid.org/0000-0002-3972-0051
Tehreema Iftikhar Government College University https://orcid.org/0000-0001-5369-0859
Faiza Saleem Lahore College for Women University https://orcid.org/0000-0001-7697-7377
Farheen Aslam Lahore College for Women University https://orcid.org/0000-0003-4380-2401

DOI:

https://doi.org/10.14393/BJ-v37n0a2021-53815

Keywords:

Aspergillus niger, Cellulase, CMCase, FPase, Molecular Characterization

Abstract

Cellulase has myriad applications in various sectors like pharmaceuticals, textile, detergents, animal feed and bioethanol production, etc. The current study focuses on the isolation, screening and optimization of fungal strain through one factor at a time technique for enhanced cellulase production. In current study sixteen different fungal cultures were isolated and the culture which quantitatively exhibits higher titers of cellulase activity was identified both morphologically and molecularly by 18S rDNA and designated as Aspergillus niger ABT11. Different parameters like fermentation medium, volume, temperature, pH and nutritional components were optimized. The highest CMCase and FPase activities was achieved in 100ml of M5 medium in the presence of 1% lactose and sodium nitrate at 30 ^oC, pH5 after 72 hours. The result revealed A. niger can be a potential candidate for scale up studies.

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