Process optimization for enhanced production of cellulases form locally isolated fungal strain by submerged fermentation




Aspergillus niger, Cellulase, CMCase, FPase, Molecular Characterization


Cellulase has myriad applications in various sectors like pharmaceuticals, textile, detergents, animal feed and bioethanol production, etc. The current study focuses on the isolation, screening and optimization of fungal strain through one factor at a time technique for enhanced cellulase production.  In current study sixteen different fungal cultures were isolated and the culture which quantitatively exhibits higher titers of cellulase activity was identified both morphologically and molecularly by 18S rDNA and designated as Aspergillus niger ABT11. Different parameters like fermentation medium, volume, temperature, pH and nutritional components were optimized. The highest CMCase and FPase activities  was achieved in 100ml of M5 medium in the presence of 1% lactose and sodium nitrate at 30 oC, pH5 after 72 hours. The result revealed A. niger can be a potential candidate for scale up studies.


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ABD-ELRSOUL, R.M.M.A. and BAKHIET, S.E.A. Optimization of factors influencing cellulase production by some indigenous isolated fungal species. Jordan Journal of Biological Sciences. 2018, 11(2), 31-36.

AKULA, S. and GOLLA, N. Optimization of cellulase production by Aspergillus niger isolated from forest soil. The open Biotechnology Journal. 2018, 12(1), 256-269.

BHAT, M. Cellulases and related enzymes in biotechnology. Biotechnology Advances. 2000, 18(5), 355-383.

BRADFORD, M.M.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Analytical Biochemistry. 1976, 72(1-2), 248-254.

CARLILE, M. J., et al. The Fungi. 2nd ed. London: Academic Press, 2001.

CHELLAPANDI, P. and JANI, H.M. Production of endoglucanase by the native strains of Streptomyces isolates in submerged fermentation. Brazilian Journal of Microbiology. 2008, 39(1), 122-127.

DA SILVA, V.C.T., et al. Effect of pH, Temperature, and Chemicals on the Endoglucanases and β-Glucosidases from the Thermophilic Fungus Myceliophthora heterothallica F. 2.1. 4. Obtained by Solid-State and Submerged Cultivation. Biochemistry Research International. 2016, 2016, 1-9.

DAMISA, D., AMEH, J. and EGBE, N. Cellulase production by native Aspergillus niger obtained from soil environments. Fermentation Technology and Bioengineering. 2011, 1, 62-70.

EL-HADI, A.A., et al. Optimization of cultural and nutritional conditions for carboxy methyl cellulase production by Aspergillus hortai, Journal of Radiation Research and Applied Sciences. 2014, 7(1), 23-28.

GAO, J., et al. Production and characterization of cellulolytic enzymes from the thermoacidophilic fungal Aspergillus terreus M11 under solid-state cultivation of corn stover. BioResource Technology. 2008, 99(16), 7623-7629.

GEOFFRY, K. and ACHUR, R.N. Screening and production of lipase from fungal organisms. Biocatalyst and Agriculture Biotechnology. 2018, 14, 241-253

GORI, M.I. and MALANA, M.A. Production of carboxymethyl cellulase from local isolate of Aspergillus species. Pakistan Journal of Life and Social Sciences. 2010, 8(1), 1-6.

HAQ, I., et al. Sugar cane bagasse pretreatment: an attempt to enhance the production potential of cellulases by Humicola insolens TAS-13. Biokemistri. 2006, 18(2), 83-88.

HAQ, I., et al. Cotton saccharifying activity of cellulases produced by co-culture of Aspergillus niger and Trichoderma viride. Research Journal of Agriculture and Biology Sciences. 2005, 1(3), 241-245.

HUSSAIN, A., et al. Cellulolytic enzymatic activity of soft rot filamentous fungi Paecilomyces variotii. Advances in BioResearch. 2012, 3(3), 10-17.

IRFAN, M., et al. UV mutagenesis of Aspergillus niger for enzyme production in submerged fermentation. Pakistan Journal of Biochemistry and Molecular Biology. 2011, 44(4), 137-140.

KARNCHANATAT, A., et al. A novel thermostable endoglucanase from the wood-decaying fungus Daldinia eschscholzii. Enzyme and Microbial Technology. 2008, 42(5), 404-413.

KARTHIKEYAN. N. and PALANI, P.S.M. Screening, identifying of Penicillium K-P strain and its cellulase producing conditions. Journal of Ecobiotechnology. 2010, 2(10), 4-7.

MMANGO-KASEKE, Z., et al. Optimization of cellulase and xylanase production bymicrococcus species under submerged fermentation. Sustainability. 2016, 8(11), 1168(1-15)

KIM, D., et al. Identification and molecular modeling of a family 5 endocellulase from Thermus caldophilus GK24, a cellulolytic strain of Thermus thermophilus. International Journal of Molecular Sciences. 2006, 7(12), 571-589.

KUMAR S., et al. MEGA X: Molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution. 2018, 35(6), 1547-1549.

LYND, L.R., et al. Microbial cellulose utilization: fundamentals and biotechnology. Microbiology and Molecular Biology Reviews. 2002, 66(3), 506-577.

MALIK, S.K., et al. Optimization of process parameters for the biosynthesis of cellulases by Trichoderma viride. Pakistan Journal Botany. 2010, 42(6), 4243-4251.

MILLER, G.L. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry. 1959, 31(3), 426-428.

NISAR, K., et al. Hyper production of carboxy methyl cellulase by Thermomyces dupontii utilizing physical and chemical mutagenesis. Revista Mexicana de Ingeniería Química. 2020, 19(2), 617-625.

OLIVEIRA, P., et al. Cocoa shell for the production of endoglucanase by Penicillium roqueforti ATCC 10110 in solid state fermentation and biochemical properties. Revista Mexicana de Ingeniería Química. 2019, 18(3), 777-787.

PADMAVATHI, T., et al. Optimization of the medium for the production of cellulases by Aspergillus terreus and Mucor plumbeus. European Journal of Experimental Biology. 2012, 2(4), 1161-1170.

POTHIRAJ, C. and EYINI, M. Enzyme activities and substrate degradation by fungal isolates on cassava waste during solid state fermentation. Mycobiology. 2007, 35(4), 196-204.

REDDY, P.L.N., et al. Screening, identification and isolation of cellulolytic fungi from soils of Chittoor district, India. International Journal of Current Microbiology and Applied Sciences. 2014, 3(7), 761-77.

SINGH, A., et al. Production of cellulases by Aspergillus heteromorphus from wheat straw under submerged fermentation. International Journal of Civil and Environmental Engineering. 2009, 1(1), 23.26.

TAO, Y.M., et al. Purification and properties of endoglucanase from a sugar cane bagasse hydrolyzing strain, Aspergillus glaucus XC9. Journal of Agricultural and Food Chemistry. 2010, 58(10), 6126-6130.

YOON, L.W., et al. Fungal solid-state fermentation and various methods of enhancement in cellulase production. Biomass and Bioenergy. 2014, 2014(67), 319–338.




How to Cite

ABDULLAH, R., AKHTAR, A., NISAR, K., KALEEM, A.., IQTEDAR, M., IFTIKHAR, T., SALEEM, F. and ASLAM, F.., 2021. Process optimization for enhanced production of cellulases form locally isolated fungal strain by submerged fermentation. Bioscience Journal [online], vol. 37, pp. e37021. [Accessed3 March 2024]. DOI 10.14393/BJ-v37n0a2021-53815. Available from:



Biological Sciences