Metal accumulation, growth and nutrition of Vernonia polyanthes exposed to lead nitrate and arbuscular mycorrhizal fungi

Joacir  Morais; Cácio Luiz  Boechat; Daniela Fernandes  de Oliveira; Adriana Miranda de Santana  Arauco; Filipe Selau  Carlos; Poliana Prates de Souza  Soares

doi:10.14393/BJ-v37n0a2021-53697

Authors

Joacir Morais Universidade Federal do Piauí https://orcid.org/0000-0001-8399-9929
Cácio Luiz Boechat Universidade Federal do Piauí https://orcid.org/0000-0002-5086-9156
Daniela Fernandes de Oliveira Universidade Federal do Rio Grande do Sul https://orcid.org/0000-0003-1816-0494
Adriana Miranda de Santana Arauco Universidade Federal do Piauí https://orcid.org/0000-0002-2538-777X
Filipe Selau Carlos Universidade Federal de Pelotas https://orcid.org/0000-0002-3068-0399
Poliana Prates de Souza Soares Universidade Estadual do Sudoeste da Bahia https://orcid.org/0000-0003-4158-4017

DOI:

https://doi.org/10.14393/BJ-v37n0a2021-53697

Keywords:

Bioaccumulation Factor, Phytoextraction, Phytostabilization, Potential Toxic Element, Translocation Factor.

Abstract

The association between plants and arbuscular mycorrhizal fungi (AMF) can be used to bioremediate areas contaminated by metals. The objectives of this work were to evaluate the lead (Pb²⁺) phytoaccumulation capacity, morpho-physiology and nutrition responses of Vernonia polyanthes exposed to a solution amended with concentrations of lead nitrate and arbuscular mycorrhizal fungi. The treatments consisted of increasing doses of Pb²⁺ as lead nitrate [Pb(NO₃)₂], two strains of AMF and an absolute control without lead and AMF. Lead negatively affected some morphophysiological variables, reduced 27.3, 25.63, 30.60, and 56.60% shoot length, root collar diameter, number of leaves and leaf area, respectively, besides reducing decreasing chlorophyll a. Lead accumulated in the shoot and roots, the latter at the highest concentrations. However, the translocation factor was above 1, indicating low efficiency. The bioaccumulation factor referring to the roots were above 1. The fungi colonization rate was low, 3.31% for Gigaspora margarita and 2.33% for Acaulospora morrowiae. However, the absorption of lead increased, reflecting in lower values of chlorophyll a, dry mass of root and diameter. Results indicated that the arboreal species V. polyanthes tolerate high concentrations of lead and can accumulate significant amounts in the roots. AMF increase the accumulation of lead in the shoot and can be used in projects aimed at the phytoextraction of metals.

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