Dehydration tolerance and storage sensitivity of Campomanesia adamantium seeds




Cerrado plants, Desiccation tolerance, Viability, Vigor.


Cultivating native fruit species depends on the existence of technical and scientific information. In this context, studies on propagation are the basis for other investigations. Campomanesia adamantium is propagated by seeds, and quality seeds guarantee the formation of high-performance plants in the field. This study aimed to analyze the dehydration tolerance and storage sensitivity of Campomanesia adamantium seeds. Experiment 1 evaluated the physiological quality of seeds with moisture contents of 43, 30, 26, 23, 18, 15, and 5%. Experiment 2 determined the seed moisture content, germination, and vigor of seeds depulped and immediately stored at 16 and 25°C, for 25 and 50 days. Seed viability was not affected by dehydration up to 15% of moisture content but decreased at 5%. Drying was accompanied by reduced seed vigor and seedling mass. The germination of unstored seeds was superior to stored ones. Storage at 16°C for 25 days was promising to maintain seed viability. The seeds showed intermediate recalcitrant behavior. Dehydration below 30% reduced vigor, while 5% affected viability. Campomanesia adamantium seeds can be stored for 25 days at 16°C in permeable packaging that maintains approximately 10% of moisture, with germination higher than 60%.


Download data is not yet available.


ALVES, E.U., et al. Comportamento fisiológico de sementes de pitombeira [Talisia esculenta (A. ST. Hil) Radlk] submetidas à desidratação. Revista Brasileira de Fruticultura. 2008, 30, 509-516.

BARBEDO, C.J., CENTENO, D.C. and RIBEIRO, R.C.L.F. Do recalcitrant seeds really exist? Hoehnea. 2013, 40(4), 583-593.

BERJAK, P. and PAMMENTER, N.W. What ultrastructure has told us about recalcitrant seeds. Revista Brasileira de Fisiologia Vegetal. 2000, 12, 22-55.

BERJAK, P. and PAMMENTER, N.W. Implications of the lack of desiccation tolerance in recalcitrant seeds. Frontiers in Plant Science. 2013, 22(4), 478.

BRASIL. Ministério da Agricultura, Pecuária e Abastecimento. Regras para análise de sementes. Ministério da Agricultura, Pecuária e Abastecimento. Secretaria de Defesa Agropecuária. Brasília: MAPA/ACS, 2009. Available from:

CASTRO, L.E., GUIMARÃES, C.C. and FARIA, J.M.R. Physiological, cellular and molecular aspects of the desiccation tolerance in Anadenanthera colubrina seeds during germination. Brazilian Journal of Biology. 2017, 77(4), 774-780.

COUTINHO, I.D., et al. Influência da variação sazonal nos teores de flavonóides e atividade antioxidante das folhas de Campomanesia adamantium (Cambess.) O. Berg. Myrtaceae. Revista Brasileira de Farmacognosia. 2010, 20(3), 322-327.

DOUSSEAU, S., et al. Ecofisiologia da germinação de sementes de Campomanesia pubescens. Ciência Rural. 2011, 41(8), 1362-1368.

DRESCH, D.M., et al. Storage of Campomanesia adamantium (Cambess.) O. Berg seeds: influence of water content and environmental temperature. American Journal of Plant Science. 2014, 5(17), 2555-2565.

DRESCH, D.M., MASETTO, T.E. and SCALON, S.P.Q. Campomanesia adamantium (Cambess.) O. Berg seed desiccation: influence on vigor and nucleic acids. Anais da Academia Brasileira de Ciências. 2015a, 87(4), 2217-2228.

DRESCH, D.M., et al Do desiccation and storage of Campomanesia adamantium (Cambess.) O. Berg. (Myrtaceae) seeds affect the formation and survival of seedlings? African Journal of Agricultural Research. 2015b, 10(33), 3216-3224.

ELLIS, R.H., HONG, T.D. and ROBERTS, E.H. An intermediate category of seed storage behavior? I. Coffee. Journal of Experimental Botany. 1990, 41(9), 1167-1174

ENDOH, K., et al. Cryopreservation of Fagus crenata seeds: estimation of optimum moisture content for maintenance of seed viability by Bayesian modeling. Canadian Journal of Forest Research. 2017, 48(2), 192-196.

FARRANT, J.M., PAMMENTER, N.W. and BERJAK, P. Recalcitrance – a current assessment. Seed Science and Technology. 1988, 16(1), 155-166.

FINCH-SAVAGE, W.E., 1996. The role of development studies in research on recalcitrant and intermediate seeds. In: QUEDRAOGO, A. S., POULSEN, K. and STUBSGAARD, F. (Ed.). Proceedings of a workshop on improved methods for handling and storage of intermediate/recalcitrant tropical forest tree seeds. Roma: International Plant Genetic Resources Institute, pp.83-97.

FLEMING, M.B., RICHARDS, C.M. and WALTERS, C. Decline in RNA integrity of dry-stores soybean seed correlates with loss of germination potential. Journal of Experimental. 2017, 68(9), 2219-2230.

FONSECA, S.C.L. and FREIRE, H.B. Sementes recalcitrantes: problemas na pós-colheita. Bragantia. 2003, 62(2), 297-303.

GOODMAN, R.C., JACOBS, D.F. and KARRFALT, R.P. Evaluating desiccation sensitivity of Quercus rubra acorns using X-ray image analysis. Canadian Journal of Forest Research. 2005, 35(12), 2823-2831.

LONG, R.L., et al. The ecophysiology of seed persistence: a mechanistic view of the journey to germination or demise. Biological Reviews of the Cambridge Philosophical Society. 2015, 90(1), 31-59.

MASETTO, T.E., et al. Desiccation tolerance and DNA integrity in Eugenia pleurantha O. Berg. (Myrtaceae) seeds. Revista Brasileira de Sementes Society. 2008, 30(1), 175-180.

MARCOS-FILHO, J. Fisiologia de sementes de plantas cultivadas. 2 ed. Londrina: Abrates, 2015.

MARTINS, C.C., et al. Secagem e armazenamento de sementes de Juçara. Revista Árvore. 2009, 33(4), 635-642.

MELCHIOR, S.J., et al. Colheita e armazenamento de sementes de gabiroba (Campomanesia adamantium Camb. – Myrtaceae) e implicações na germinação. Revista Brasileira de Sementes. 2006, 28(3), 141-150.

MICHALAK, M., et al. Desiccation tolerance and cryopreservation of seeds of black poplar (Populus nigra L.), a disappearing tree species in Europe. European Journal of Forest Research. 2015, 134(1), 53-60.

OSBORNE, D.J. Hazards of a germinating seed: available water and the maintenance of genomic integrity. Israel Journal of Plant Sciences. 2000, 48(3), 173-179. ISSN 0792-9978.

PAMMENTER, N.W. and BERJAK, P. Aspects of recalcitrant seed physiology. Revista Brasileira de Fisiologia Vegetal. 2000, 12, 56-96. Available from:

PAMMENTER, N.W., BERJAK, P. and WALTERS, C., 2000. The effect of drying rate on recalcitrant seeds. “lethal water content” causes of damage, and quantification of recalcitrance. In: BLACK, M. and BRADFORD, K. J. and VÁZQUEZ-RAMOS, J. (Ed.). Seed biology – advances and applications. New York: CABI Publishing, pp. 21-221.

PÉREZ, H.E., HILL, L.M. and WALTERS, C. An analysis of embryo development in palm: interactions between dry matter accumulation and water relations in Pritchardia remota (Arecaceae). Seed Science Research. 2012, 22(2), 97-111.

SACANDÉ, M., et al. (Eds.) Comparative storage biology of tropical tree seeds. Roma: International Plant Genetic Resources Institute, 2004.

SANTOS, P.C.G., et al. Qualidade de sementes de Hancornia speciosa Gomes em função do tempo de secagem. Semina: Ciências Agrárias. 2010, 31(2), 343-352. Available from:

SCALON, S.P.Q., et al. Sensibilidade à dessecação e ao armazenamento em sementes de Eugenia pyriformis Cambess. (uvaia). Revista Brasileira de Fruticultura. 2012, 34(1), 269-276.

VALLILO, M.I., et al. Composição química dos frutos de Campomanesia adamantium (Cambessedes) O. Berg. Food Science and Technology. 2006, 26(4), 805-810.

VIEIRA, C.V., et al. Germinação e armazenamento de sementes de caboatã (Cupania vernalis Cambess.) – Sapindaceae. Ciência Agrotecnológica. 2008, 32(2), 444-449.

WALTERS, C. Levels of recalcitrance in seeds. Revista Brasileira de Fisiologia Vegetal. 2000, 2, 7-21.

WALTERS, C. Orthodoxy, recalcitrance and in-between: describing variation in seed storage characteristics using threshold responses to water loss. Planta. 2015, 242(2), 397-406.

WYSE, S.V., DICKIE, J.B. and DYER, A. Predicting the global incidence of seed desiccation sensitivity. Journal of Ecology. 2017, 105, 1082-1093.




How to Cite

FERNANDES LEÃO-ARAÚJO, Érica, APARECIDA SILVEIRA FERREIRA, E., AGUIAR SILVA, M., LÚCIA COSTA, L., PEIXOTO, N. and REGINA BARBOZA DE SOUZA, E., 2022. Dehydration tolerance and storage sensitivity of Campomanesia adamantium seeds. Bioscience Journal [online], vol. 38, pp. e38069. [Accessed24 July 2024]. DOI 10.14393/BJ-v38n0a2022-53571. Available from:



Agricultural Sciences