Characterization and plasticity of wharton's jelly mesenchymal stem cells of goat

Gustavo Cardoso da Silva  Neves; Napoleão Martins Argôlo Neto; Maíra Soares  Ferraz; Clautina Ribeiro de Moraes  da Costa; Andressa Rêgo  da Rocha; Huanna Waleska Soares Rodrigues; Felipe de Carvalho  Nunes; Camila Ernanda Sousa  de Carvalho; Miguel Ferreira  Cavalcante Filho; Danilo José Ayres  de Menezes; Maria Acelina Martins de Carvalho

doi:10.14393/BJ-v37n0a2021-50386

Authors

Gustavo Cardoso da Silva Neves Universidade Federal do Piauí https://orcid.org/0000-0001-5488-1163
Napoleão Martins Argôlo Neto Universidade Federal do Piauí https://orcid.org/0000-0002-6218-6168
Maíra Soares Ferraz Universidade Federal do Piauí https://orcid.org/0000-0002-3162-1729
Clautina Ribeiro de Moraes da Costa Instituto Federal de Educação, Ciência e Tecnologia do Piauí https://orcid.org/0000-0001-7512-4540
Andressa Rêgo da Rocha Instituto Federal de Educação, Ciência e Tecnologia do Piauí https://orcid.org/0000-0003-3586-3482
Huanna Waleska Soares Rodrigues Universidade Federal do Piauí https://orcid.org/0000-0002-0329-090X
Felipe de Carvalho Nunes Centro Universitário Maurício de Nassau https://orcid.org/0000-0001-8089-331X
Camila Ernanda Sousa de Carvalho Universidade Federal do Piauí https://orcid.org/0000-0002-6928-7527
Miguel Ferreira Cavalcante Filho Universidade Federal do Piauí https://orcid.org/0000-0002-7319-9079
Danilo José Ayres de Menezes Universidade Federal do Rio Grande do Norte https://orcid.org/0000-0001-6089-3283
Maria Acelina Martins de Carvalho Universidade Federal do Rio Grande do Norte https://orcid.org/0000-0001-9165-7339

DOI:

https://doi.org/10.14393/BJ-v37n0a2021-50386

Keywords:

Cell Culture, Flow Cytometry, Lineage Differentiation, Mesenchymal Stem Cells, Umbilical Cord Matrix.

Abstract

Mesenchymal stem cells (MSCs), obtained from several anatomical sites, have already been described, characterized and used in therapeutic models for tissue repair. The umbilical cord mesenchymal stem cells, represented by cells from arteries and veins walls, as well as Wharton's jelly are easy to be obtained, highly available, require no invasive procedure, do not present risk to donors and do not present ethical limitation. The aim of this research was to analyze the plasticity of Wharton's jelly mesenchymal stem cells (WJ-MSCs) of goat, evaluating their behavior in vitro and characterizing them immunophenotypically. Thus, tests were performed on colony forming units, viability and cell growth curve, flow cytometry analysis and plasticity potential. Goat umbilical cord matrix cells exhibited fibroblastoid morphology with colony formation and self-renewal ability, always maintaining their undifferentiated state up to the eighth passage (P8). The growth curve kinetics exhibited the LAG, LOG, and DECAY phases, without displaying a PLATEAU phase. The plasticity assay demonstrated positive differentiation for osteogenic, adipogenic and chondrogenic lines, characterized by the synthesis of intracytoplasmic granules or extracellular matrix with the presence of calcium, lipids and proteoglycans. Flow cytometry demonstrated the expression of CD90 and CD105; absence of CD14 expression. It is concluded that the cell population isolated from the Wharton's jelly of goat constitutes a representative sample of mesenchymal stem cells, with great possibilities in the field of regenerative and reproductive medicine.

Downloads

Download data is not yet available.

References

ARGÔLO NETO, N.M., et al. Isolation, expansion, differentiation and growth kinetics essay in mesenchymal stem cells culture from the bone marrow of collared peccaries (Tayassu tajacu). Acta Scientiae Veterinariae. 2016, 44(1), 1-11. https://doi.org/10.22456/1679-9216.80850

BASTAWROUS, M., et al., 2016. Wharton’s jelly stem cells. In: FAUZA, D.O. and BANI, M., eds. Fetal Stem Cells in Regenerative Medicine. New York: Springer, pp. 257-276. https://doi.org/10.1007/978-1-4939-3483-6

COSTA, C.R.M., et al. Labeling of adipose-derived stem cells with quantum dots provides stable and long-term fluorescent signal for ex vivo cell tracking. In Vitro Cellular & Developmental Biology-Animal. 2017, 53(4), 363-370. https://doi.org/10.1007/s11626-016-0121-2

DA ROCHA, A.R., et al. Hematopoietic progenitor constituents and adherent cell progenitor morphology isolated from black‐rumped agouti (Dasyprocta prymnolopha, Wagler 1831) bone marrow. Microscopy Research and Technique. 2012, 75(10), 1376-1382. https://doi.org/10.1002/jemt.22077

DE SCHAUWER, C., et al. Markers of stemness in equine mesenchymal stem cells: a plea for uniformity. Theriogenology. 2011, 75(8), 1431-1443. https://doi.org/10.1016/j.theriogenology.2010.11.008

DESANCÉ, M., et al. Chondrogenic differentiation of defined equine mesenchymal stem cells derived from umbilical cord blood for use in cartilage repair therapy. International Journal of Molecular Sciences. 2018, 19(2), 537. https://doi.org/10.3390/ijms19020537

DONDERS, R., et al. Human Wharton's Jelly-derived stem cells display a distinct immunomodulatory and proregenerative transcriptional signature compared to bone marrow-derived stem cells. Stem Cells and Development. 2018, 27(2), 65-84. https://doi.org/10.1089/scd.2017.0029

DONOFRIO, G., et al. Susceptibility of bovine mesenchymal stem cells to bovine herpesvirus 4. Journal of Virological Methods. 2005, 127(2), 168-170, 2005. https://doi.org/10.1016/j.jviromet.2005.02.019

EL-ASHMAWY, N.E., et al. Effect of human umbilical cord blood-derived mononuclear cells on diabetic nephropathy in rats. Biomedicine & Pharmacotherapy. 2018, 97, 1040-1045. https://doi.org/10.1016/j.biopha.2017.10.151

GAUTHAMAN, K., et al. Human umbilical cord Wharton's jelly stem cell (hWJSC) extracts inhibit cancer cell growth in vitro. Journal of Cellular Biochemistry. 2012, 113(6), 2027-2039. https://doi.org/10.1002/jcb.24073

GUGJOO, M.B., et al. Goat mesenchymal stem cell basic research and potential applications. Small Ruminant Research. 2020, 183, 106045. https://doi.org/10.1016/j.smallrumres.2019.106045

MOHAMMADI, M., et al. Effect of gestational age on migration ability of the human umbilical cord vein mesenchymal stem cells. Advances in Medical Sciences. 2018, 63(1), 119-126. https://doi.org/10.1016/j.advms.2017.08.002

MOSHREFI, M., et al. Isolation and characterization of mesenchymal cells isolated from caprine umbilical cord matrix. Animal Reproduction. 2018, 7(4), 367-372.

PRIETO, P., et al. Cell expansion-dependent inflammatory and metabolic profile of human bone marrow mesenchymal stem cells. Frontiers in Physiology. 2016, 7, 548. https://doi.org/10.3389/fphys.2016.00548

PURANIK, S.B., et al. Isolation of mesenchymal-like cells from Wharton’s jelly of umbilical cord. International Journal of Pharmaceutical, Chemical and Biological Sciences. 2012, 2(3), 218-224.

ROCHA, A.R., et al. Immunophenotyping, plasticity tests and nanotagging of stem cells derived from adipose tissue of wild rodent agouti (Dasyprocta prymnolopha). Arquivo Brasileiro de Medicina Veterinária e Zootecnia. 2019, 71(5), 1571-1581. https://doi.org/10.1590/1678-4162-10677

ROCHA, M.A.C., et al. Allogeneic mesenchymal stem cells and xenogenic platelet rich plasma, associated or not, in the repair of bone failures in rabbits with secondary osteoporosis. Acta Cirurgica Brasileira. 2017, 32(9), 767-780. http://dx.doi.org/10.1590/s0102-865020170090000009

SARGIACOMO, C., et al. Upregulation of sodium taurocholate cotransporter polypeptide during hepatogenic differentiation of umbilical cord matrix mesenchymal stem cells facilitates hepatitis B entry. Stem Cell Research & Therapy. 2017, 8(1), 204. https://doi.org/10.1186/s13287-017-0656-5

SIRONI, F., et al. Multiple intracerebroventricular injections of human umbilical cord mesenchymal stem cells delay motor neurons loss but not disease progression of SOD1G93A mice. Stem Cell Research. 2017, 25, 166-178. https://doi.org/10.1016/j.scr.2017.11.005

SOARES, I.M.V. et al. The influence of Aloe vera with mesenchymal stem cells from dental pulp on bone regeneration: characterization and treatment of non-critical defects of the tibia in rats. Journal of Applied Oral Science. 2019, 27, e20180103. https://doi.org/10.1590/1678-7757-2018-0103

TAGHIZADEH, R.R.; CETRULO, K.J. and CETRULO, C.L. Wharton’s Jelly stem cells: future clinical applications. Placenta. 2011, 32, S311-S315. https://doi.org/10.1016/j.placenta.2011.06.010

VOLAREVIC, V., et al. Stem cells as new agents for the treatment of infertility: current and future perspectives and challenges. BioMed Research International. 2014, 2014, 507234. https://doi.org/10.1155/2014/507234

XIE, J., et al. Umbilical cord-derived mesenchymal stem cells alleviated inflammation and inhibited apoptosis in interstitial cystitis via AKT/mTOR signaling pathway. Biochemical and Biophysical Research Communications. 2018, 495(1), 546-552. https://doi.org/10.1016/j.bbrc.2017.11.072