Effects of Stryphnodendron adstringens extracts on murine 4T1 tumor line

Alessandra Aparecida de Melo  Souza; Elias Raad  Gervásio; Thais Barbosa  de Paula; Luis Ribeiro da Silva Neto; Fernanda Pinheiro Chagas  Fernandes; Gabriel Furtado  Leite; Filipe Peres  Barreto; Rosy Iara Maciel de Azambuja Ribeiro

doi:10.14393/BJ-v37n0a2021-50347

Authors

Alessandra Aparecida de Melo Souza Universidade Federal de São João Del Rei https://orcid.org/0000-0002-7380-0653
Elias Raad Gervásio Universidade Federal de São João Del Rei https://orcid.org/0000-0003-1915-2242
Thais Barbosa de Paula Universidade Federal de São João Del Rei https://orcid.org/0000-0002-8761-1108
Luis Ribeiro da Silva Neto Universidade Federal de São João Del Rei https://orcid.org/0000-0001-7064-8943
Fernanda Pinheiro Chagas Fernandes Universidade Federal de São João Del Rei https://orcid.org/0000-0002-9031-8375
Gabriel Furtado Leite Universidade Federal de São João Del Rei https://orcid.org/0000-0002-9490-6238
Filipe Peres Barreto Universidade Federal de São João Del Rei https://orcid.org/0000-0001-6905-0338
Rosy Iara Maciel de Azambuja Ribeiro Universidade Federal de São João Del-Rei https://orcid.org/0000-0002-7380-0653

DOI:

https://doi.org/10.14393/BJ-v37n0a2021-50347

Keywords:

Carcinoma, Cell Culture, Cerrado Plants, Cytotoxicity, Stryphnodendron adstringens.

Abstract

Breast cancer appears as the main gynecological cancer and presents high morbidity and mortality. Because most diagnoses are made belatedly, it is necessary to seek therapeutic options that aim for advanced stages of the disease. This study aims to evaluate the antitumoral action of Stryphnodendron adstringens fruit extracts on 4T1 murine mammary carcinoma cell culture. The inhibitory potential of S. adstringens fruit extract on the metalloproteinases (MMPs) 2 and 9 was evaluated through zymography. From these results, MTT assays were performed to evaluate the extracts’ effects on the murine mammary carcinoma 4T1 line cell viability. From the crude extract, the following extracts were obtained: hydroalcoholic (SAFCEA), hexane (SAFCEB), chloroform (SAFCEC), and ethyl acetate (SAFCED). Lastly, the migration of the cells treated with extracts SAFCEA and SAFCED was verified. The hydroalcoholic extract (SAFCEA) was the most efficient in inhibiting gelatinases. During the phytochemical study, it was noted that alkaloids were present in all partitions. The 50 % growth inhibition (IC₅₀) concentrations found were: 40.1 μg/mL (SAFCEA) and 70.14 μg/mL (SAFCED). After the cellular cytotoxicity assay, cell morphology was altered by treatment with the selected partitions (SAFCEA and SAFCED), obtaining morphology consistent with apoptosis. The results demonstrate that S. adstringens extracts exhibit the inhibitory activity of MMP-2 and MMP-9 as well as cytotoxicity toward 4T1 tumor cells. These findings indicate that follow-up studies of the partitions from S. adstringens may lead to the development of novel chemotherapeutics for oncological treatments.

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