Antagonistic activity of bacteria from wild honey against Colletotrichum musae, and testing of wild honey as biopesticide spray to control banana anthracnose

Authors

  • Mark Paul Selda Rivarez University of the Philippines Los Baños
  • Elizabeth P. Parac Caraga State University https://orcid.org/0000-0002-1987-9612
  • Niño R. Laurel University of the Philippines Los Baños
  • Benjamin V. Cunanan University of the Philippines Los Baños, Los Baños
  • Angelie B. Magarro University of the Philippines Los Baños
  • Shajara Fatima M. Dimasingkil Mindanao State University-Maguindanao

DOI:

https://doi.org/10.14393/BJ-v37n0a2021-49921

Keywords:

Bacillus, Banana Anthracnose, Biological Control, Colletotrichum musae, Lactobacillus, Wild Honey.

Abstract

Anthracnose is a foliar and fruit disease caused by Colletotrichum spp. affecting a wide range of crops. Infection occurs early followed by quiescence in fruits, such as in banana, where chemical-based pesticides are used as a dependable fungal control for many years. There is an increasing need for a safe control and as implicated in the Organic Agriculture Act of 2010 (RA 10068) in the Philippines. This scenario drove the use of alternative pest control such as the use of biologicals and natural products. In this study, seven bacteria were isolated from wild honey, produced by Apis mellifera, wherein four (BC2, BC3, BC6 and BC7) were found to be an effective antagonist against Colletotrichum musae in in vitro conditions. These bacteria were identified to belong to the genus Lactobacillus spp. (BC2, BC3, BC7) and Bacillus spp. (BC6) based on sugar utilization tests, morphological and cultural growth in PDPA. For the in vivo test, different dilutions of wild honey were used and it was found out that lower concentrations were effective as biopesticide spray to prevent anthracnose infection. Lastly, we report herewith the first isolation of bacteria with biological control potential from wild honey, and to apply the raw or natural product as biopesticide in postharvest fruits.

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References

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Published

2021-12-29

How to Cite

RIVAREZ, M.P.S.., PARAC, E.P., NIÑO R. LAUREL, BENJAMIN V. CUNANAN, ANGELIE B. MAGARRO and DIMASINGKIL, S.F.M., 2021. Antagonistic activity of bacteria from wild honey against Colletotrichum musae, and testing of wild honey as biopesticide spray to control banana anthracnose. Bioscience Journal [online], vol. 37, pp. e37089. [Accessed14 August 2022]. DOI 10.14393/BJ-v37n0a2021-49921. Available from: https://seer.ufu.br/index.php/biosciencejournal/article/view/49921.

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Section

Agricultural Sciences