Extracts of native forest species of the southern amazon in the control of Aphis craccivora koch (Hemiptera: aphididae)





Aphid, Insecticide Botanic, Plant Extracts.


The objective of this study was to evaluate the mortality of Aphis craccivora Koch (Hemiptera: Aphididae) submitted to different extracts with different concentrations of leaves of the native forest species: Tetragastris altissima (Aubl.) Swart (Burseraceae), Metrodorea flavida K. Krause (Rutaceae) and Cheiloclinium cognatum (Miers) AC Sm (Celastraceae) under laboratory conditions. Adults of Aphis craccivora were collected in Gliricidia sepium (Jacq.) Kunthe Walp. (Fabaceae), and separated into groups of ten individuals, transferred to Petri dishes and exposed to topical application of aqueous, alcoholic and leaf infusion extracts of Cheiloclinium cognatum, Metrodorea flavida and Tetragastris altissima. The extracts were applied at concentrations of: 3, 6, 9, 12 and 15%, with 1% dimethylsulfoxide (DMSO), using a control treatment 1 (distilled water + DMSO 1%) and control treatment 2 (fipronil). Evaluations were performed at 24, 48 and 72 hours after the application of the extracts by counting the dead insects. The experimental design was completely randomized, with five replicates, three types of extracts and five concentrations with controls. In all the studied species, the aqueous and infusion extracts presented a lower mortality (less than 52%), whereas the alcoholic extract in higher concentrations of Cheiloclinium cognatum, Metrodorea flavida showed a mortality of up to 100%. The species Tetragastris altissima reached an average mortality of 92%. The lethal concentration for the alcoholic extract of Cheiloclinium cognatum was LC50 6.43% and LC90 12.22%, Metrodorea flavida LC50 was 3.08% and LC90 7.05% and that for Tetragastris altissima LC50 5.58% and LC90 17.47%, after 72 hours. The use of the alcoholic extract of the species Metrodorea flavida at a concentration of 9% in the control of Aphis craccivora is indicated.


Download data is not yet available.


ABBOTT, W.S. A method for computing the effectiveness of an insecticide. Journal of Invertebrate Pathology. 1925, 18, 265-267.

AGROFIT - Sistema de Agrotóxicos Fitossanitários. Inseticidas registrados para controle de Aphis craccivora. Available from: http://agrofit.agricultura.gov.br/agrofit_cons/principal_agrofit_cons

ANDREO, D. and JORGE, N. Antioxidantes naturais: técnicas de extração. Boletim do Centro de Pesquisa de Processamento de Alimentos. 2006, 24(2), 319-336. http://dx.doi.org/10.5380/cep.v24i2.7489

BANDEIRA, H.F.S., et al. Preferência do pulgão-preto e da cigarrinha-verde em diferentes genótipos de feijão caupi em Roraima. Revista Agro@mbiente. 2015, 9(1), 79-85. http://dx.doi.org/10.18227/1982-8470ragro.v9i1.1998

BANDEIRA, H.F., et al. Bioactivity of Annona Montana Macfad extracts on the black cowpea aphid (Aphiscraccivora Koch). Revista Brasileira de Ciências Agrárias. 2017, 12(1), 41-46. http://dx.doi.org/10.5039/agraria.v12i1a5419

BARAKAT, D.A. Insecticidal and antifeed ant activities and chemical composition of Casimiroa edulis La Llave & Lex (Rutaceae) leaf extract and its fractions against Spodoptera littoralis larvae. Australian Journal of Basic and Applied Sciences. 2011, 5(9), 693-703.

BASKAR, K., et al. Ovicidal activity of Atalantia monophylla (L) Correa against Spodoptera litura Fab. (Lepidoptera: Noctuidae). Asian Pacific journalof tropical biomedicine. 2012, 2(12), 987-991. http://dx.doi.org/10.1016/S2221-1691(13)60011-8.

CAMARGO, R.S., et al., 2011. Morfologia interna. In: FUJIHARA, R. T. et al., eds. Insetos de importância econômica: guia ilustrado para identificação de famílias. Botucatu: Fepaf., pp. 43-61.

CARVALHO, G.A., et al. Eficiência do óleo de nim (Azadirachta indica A. Juss) no controle de Brevicoryne brassicae (Linnaeus, 1758) e Myzus persicae (Sulzer, 1776) (Hemiptera: Aphididae) em couve-manteiga Brassica oleracea Linnaeus var. Acephala. Arquivos do Instituto Biológico. 2008, 75(2), 181-186.

CARVALHO, G.S., et al. Mortalidade e comprometimento do desenvolvimento de Zabrotes subfasciatus Boh. (Coleoptera: Chrysomelidae), induzido pelo extrato de sangra d'água Croton urucurana Baill (Euphorbiaceae). Comunicata Scientiae. 2014, 5(3), 331-338. https://doi.org/10.14295/cs.v5i3.471

CARVALHO, J.R., et al. Análise de probit aplicada a bioensaios com insetos. Colatina: IFES, 2017.

CUNHA, S.B.Z., et al. Predators of the Alfalfa Aphis Acyrthosiphon pisum (Harris), Aphis craccivora Koch, and Therioaphis trifolii (Monell) (Hemiptera: Aphidoidea) as determined by the Serological Technique. EntomoBrasilis. 2016, 9(2), 120-123. http://dx.doi.org/10.12741/ebrasilis.v9i2.595

FAZOLIN, M., et al., 2016. Feijão. In: SILVA, N.M., ADAIME, R. and ZUCCHI, R.A., eds. Pragas Agrícolas e Florestais na Amazônia. Brasilia, DF: Embrapa, pp. 323-343.

FERREIRA, E.B., CAVALCANTI, P.P. and NOGUEIRA, D.A. ExpDes.pt: Experimental Designs package (Portuguese). R packageversion 1.1.2, 2013.

FINNEY, D. J. Probit Analysis, 3rd. ed. London: Cambridge University Press, 1971.

FONSECA, J., et al. Efeito de extratos metanólicos de Stryphnodendron adstringens (Mart) coville na alimentação e reprodução de Plutella xylostella L. (Lepidoptera: Plutellidae). Interciencia. 2018, 43(3), 182-187.

GADIR, A. and AHMED, I.M. Commiphora myrrha and Commiphora african a essential oils. Journal of Chemical and Pharmaceutical Research. 2014, 6(7), 151-156.

GALLO, D., et al. Entomologia Agrícola. Piracicaba: FEALQ, 2002.

GAMA, J. Ecotoxicology: methods for ecotoxicology. R packageversion 1.0.1, 2015.

GHANIM, N.M. and GHANI, S.B.A. Controlling Tuta absoluta (Lepidoptera: Gelechiidae) and Aphis gossypii (Hemiptera: Aphididae) by aqueous plant extracts. Life Science Journal. 2014, 11(3), 299-307.

KRINSKI, D., MASSAROLI, A. and MACHADO, M. Potencial inseticida de plantas da família Annonaceae. Revista Brasileira de Fruticultura. 2014, 36(1), 225-242. http://dx.doi.org/10.1590/S0100-29452014000500027

KRISHNAMOORTHY, S., et al. Identification of chemical constituents and larvicidal activity of essential oil from Murraya exotica L. (Rutaceae) against Aedes aegypti, Anopheles stephensi and Culex quinquefasciatus (Diptera: Culicidae). Parasitology Research. 2015, 114(5), 1839-1845. http://dx.doi.org/10.1007/s00436-015-4370-x

KRISHNAPPA, K. and ELUMALAI, K. Larvicidal and ovicidal activities of Chloroxylons wietenia (Rutaceae) essential oils against Spodoptera litura (Lepidoptera: Noctuidae) and their chemical compositions. International Journal of Current Research in Life Sciences. 2012, 1(1), 3-7.

LAAMARI, M., KHELFA, L. and D’ACIER, A.C. Resistance source to cowpea aphid (Aphis craccivora Koch) in broad bean (Vicia faba L.) Algerian landrace collection. African Journal of Biotechnology. 2008, 7(14), 2486-2490. http://dx.doi.org/10.4314/ajb.v7i14.59055

LAARIF, A., et al. Chemical composition and insecticidal activity of essential oil from Citrus aurantium (Rutaceae) fruit peels against two green house insects; Spodoptera littoralis (Noctuidae) and Tuta absoluta (Gelechiidae). Advances in Agriculture, Sciences and Engineering Research. 2013, 3(5), 825-830.

LATINOVIC, N., KARAMAOUNA, F. and KAVALLIERATOS, N.G. First record of Aphis craccivora Koch (Hemiptera: Aphididae) on aronia crop in Montenegro. Hellenic Plant Protection Journal. 2017, 10(2), 67-69. http://dx.doi.org/10.1515/hppj-2017-0007

LEITE, D.M., GARLET, J. and KARSBURG, I.V. First report of the Aphis craccivora Koch on Catasetum sp. in Brazil. EntomoBrasilis. 2017, 10(3), 251-253. http://dx.doi.org/10.12741/ebrasilis.v10i3.701

LOIZZO, M.R., et al. Ruta chalepensis L. (Rutaceae) leaf extract: chemical composition, antioxidant and hypoglicaemic activities. Natural product research. 2018, 32(5), 521-528. http://dx.doi.org/10.1080/14786419.2017.1326491

MELVILLE, C.C., et al. Preferência do pulgão-preto, Aphis craccivora Koch (Hemiptera: Aphididae), a genótipos de feijão-caupi. Revista Agroambiente. 2016, 10(2), 153-160. http://dx.doi.org/10.18227/1982-8470ragro.v10i2.3042

NAVARRO-SILVA, M.A., MARQUES, F.A. and DUQUE, J.E.L. Review of semiochemicals that mediate the oviposition of mosquitoes: a possible sustainable tool for the control and monitoring of Culicidae. Revista Brasileira de Entomologia. 2009, 53(1), 1-6. http://dx.doi.org/10.1590/S0085-56262009000100002

PINA, E.S., et al. Improved production of quinone-methide triterpenoids by Cheiloclinium cognatum root cultures: possibilities for a non-destructive biotechnological process. Plant Cell, Tissue and Organ Culture (PCTOC). 2017, 128(3), 705-714. http://dx.doi.org/10.1007/s11240-016-1151-x

R DEVELOPMENT CORE TEAM. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria, 2017. Available from: http://www.Rproject.org

RABELO, J.S. and BLEICHER, E. Controle de pulgão-preto em feijão-caupi com o uso de sementes de Annonaceae e a bioatividade das sementes em diferentes épocas de armazenamento. Agropecuária científica no Semi-Árido. 2014, 10(4), 5-8. http://dx.doi.org/10.30969/acsa.v10i4.603

RIZVI, R., et al. Effect of some botanicals for the management of plant-parasitic nematodes and soil-inhabiting fungi infesting chickpea. Turkish Journal of Agriculture and Forestry. 2012, 36(6), 710-719. http://dx.doi.org/10.3906/tar-1201-45

RODRIGUES, S.R., et al. Preferência do pulgão preto Aphis craccivora Koch, 1854 (Hemiptera: Aphididae) por Genótipos de Feijão-Caupi Vignaun guiculata (L.) Walp. (Fabaceae). Bioscience Jounal. 2012, 28(5), 30-32.

RUDIGER, A.L., SIANI, A.C. and VEIGA Jr., V.F. The Chemistry and Pharmacology of the South America genus Protium Burm. f. (Burseraceae). Pharmacognosy Review. 2007, 1(1), 93-104.

SILVA, F.C., DUARTE, L.P. and VIEIRA FILHO, S.A. Celastráceas: fontes de triterpenos pentacíclicos com potencial atividade biológica. Revista Virtual de Química. 2014, 6(5), 1205-1220. http://dx.doi.org/10.5935/1984-6835.20140079

SILVA, J.P.G.F., et al. Repelência e deterrência na oviposição de Bemisia tabaci biótipo B pelo uso de extratos vegetais em Cucurbita pepo L. Revista Brasileira de Plantas Medicinais, 14(1), 76-83. http://dx.doi.org/10.1590/S1516-05722012000100011

SILVA, S.C.S., et al. Perfil fitoquímico, susceptibilidade antibacteriana e capacidade antioxidante das folhas de Croton urucurana Baillon (Euphorbiaceae). Infarma-Ciências Farmacêuticas. 2017a, 29(3), 264-270. http://dx.doi.org/10.14450/2318-9312.v29.e3.a2017.pp264-270

SILVA, T.S.A., et al. Potencial inseticida de plantas medicinais encontradas na Amazônia Central contra o pulgão-da-couve Brevicoryne brassicae (L.) (Hemiptera: Aphididae). EntomoBrasilis. 2017b, 10(2), 106-111. http://dx.doi.org/10.12741/ebrasilis.v10i2.697

SPIVEY, A.C., WESTON, M. and WOODHEAD, S. Celastraceae sesquiterpenoids: biological activity and synthesis. Chemical Society Reviews. 2002, 31(1), 43-59. http://dx.doi.org/10.1039/b000678p

SPLETOZER, A.G., SOARES-LOPES, C.R.A. and PERON, M. Estrutura populacional e análise fitoquímica de Metrodorea flavida k. Krause (Rutaceae) em dois fragmentos florestais do município de Alta Floresta, Mato Grosso. Enciclopédia Biosfera. 2015, 11(22), 1194-1205.

STURZA, S.V.S., et al. Primeiro registro de Aphis craccivora Koch 1854 (Hemiptera: Aphididae) sobre plantas daninhas em Santa Maria, RS. Ciência Rural. 2011, 41(11), 1863-1866. http://dx.doi.org/10.1590/S0103-84782011001100001

TAGLIARI, M.S., KNAAK, N. and FIUZA, L.M. Efeito de extratos de plantas na mortalidade de lagartas de Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae). Arquivos do Instituto Biológico. 2010, 77(2), 259-264.

VIEGAS Jr, C. Terpenos com atividade inseticida: uma alternativa para o controle químico de insetos. Química Nova. 2003, 26(3), 390-400. http://dx.doi.org/10.1590/S0100-40422003000300017




How to Cite

SANCHES, L.A., OLIVEIRA, S. de B., SASAYA, M.K. and GARLET, J., 2021. Extracts of native forest species of the southern amazon in the control of Aphis craccivora koch (Hemiptera: aphididae). Bioscience Journal [online], vol. 37, pp. e37018. [Accessed14 August 2022]. DOI 10.14393/BJ-v37n0a2021-48182. Available from: https://seer.ufu.br/index.php/biosciencejournal/article/view/48182.



Agricultural Sciences