Enhanced production of β- glucosidase by locally isolated fungal strain employing submerged fermentation

Authors

  • Roheena Abdullah Lahore college for women university
  • Sobia Nazir Chudhary Lahore college for women university
  • Afshan Kaleem Lahore college for women university
  • Mehwish Iqtedar Lahore college for women university
  • Kinza Nisar
  • Tehreema Iftikhar Lahore college for women university
  • Faiza Saleem Lahore college for women university

DOI:

https://doi.org/10.14393/BJ-v35n5a2019-47943

Keywords:

β-glucosidase., Aspergillus niger., Fungi., Submerged fermentation.

Abstract

β-glucosidase has wide spectrum of biotechnological applications in different industries including food, textile, laundry detergents, pulp and paper, pharmaceutical and biofuel industry. The present investigation related to isolation, screening, and process optimization of fungal strain for enhanced production of β-glucosidase (BGL). For this purpose, different fungal stains were isolated from different sources including soil, fruits, bark of tree as well as from the compost. The screening of fungal strain for BGL production was carried out via submerged fermentation. All the tested strains were identified on the basis of micro and macroscopic features. The fungal strain having greater ability for BGL synthesis among tested ones was identified as Aspergillus niger and given the code SBT-15. The process parameter including fermentation media, temperature, pH, rate of fermentation, carbon and nitrogen sources, volume of media were optimized. Five different fermentation media were evaluated M3 medium gave maximum production. The optimal conditions for BGL production was 72 hours of incubation at 40°C, pH 6 and 50 ml fermentation medium. Glucose (1%) and ammonium sulphate (3%) were optimized as best carbon and nitrogen sources, respectively.

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Published

2019-10-09

How to Cite

ABDULLAH, R., NAZIR CHUDHARY, S., KALEEM, A., IQTEDAR, M., NISAR, K., IFTIKHAR, T. and SALEEM, F., 2019. Enhanced production of β- glucosidase by locally isolated fungal strain employing submerged fermentation. Bioscience Journal [online], vol. 35, no. 5, pp. 1552–1559. [Accessed28 May 2022]. DOI 10.14393/BJ-v35n5a2019-47943. Available from: https://seer.ufu.br/index.php/biosciencejournal/article/view/47943.

Issue

Section

Biological Sciences