Evaluation of response to anti-leptospira bacterin vaccination in pregnant ewes and the passive transfer of antibodies to their offspring

Authors

  • Ligia Pinho Cuccato Universidade Federal de Uberlândia
  • Anna Monteiro Correia Lima Universidade Federal de Uberlândia
  • Thais Fernanda Martins dos Reis Universidade Federal de Uberlândia
  • Caroline Lopes Queiroz Universidade Federal de Uberlândia
  • Ana Beatriz Gacez Buiatte Universidade Federal de Uberlândia
  • Andrea Zago Ciuffa Universidade Federal de Uberlândia
  • Dayane Olímpia Gomes Universidade Federal de Uberlândia
  • Vinícius Buiatte de Andrade Alves Universidade Federal de Uberlândia
  • Fernanda Rosalinski-Moraes Universidade Federal de Uberlândia

DOI:

https://doi.org/10.14393/BJ-v36n5a2020-47708

Keywords:

Immunization., Leptospirosis., Sheep.

Abstract

Leptospirosis in sheep is often underestimated, and leads to great economic losses for the sheep farming industry. The aim of this study was to evaluate the humoral immune response in pregnant ewes, after the injection of a commercial polyvalent vaccine for leptospirosis, and to observe the transmission of anti-Leptospira antibodies through the colostrum to the offspring. For this, 24 pregnant ewes were vaccinated for leptospirosis.  Blood samples were collected prior to vaccination and then 7, 14, 21, 28, 35, 42 and 49 days after vaccination. In order to evaluate passive immunity transfer, blood samples of 32 lambs were collected during the first 48 hours after birth, and another collection was performed 10 to 21 days after birth. The lambs were placed into 2 groups: Group A (n=16): singleton lambs; and group B (n=16): twins. The sera samples were submitted to the Microscopic Agglutination Test (MAT), in which 21 Leptospira serovars were tested. The results were analyzed in a descriptive form. The number of sheep reactive to MAT gradually increased until 21 days after vaccination, and decreased right after. Of all the serovars contained in the vaccine, the largest proportion of animals were seroconverted to Hardjoprajtino serovar, Serjoe serogroup. Anti-Leptospira antibodies transferred through colostrum to lambs were detected by MAT in the serum collected 24-48 hours after birth.  It was observed that 65.6% (21 out of 32) of the lambs were reactive. In the subsequent collections that occurred from 10 to 21 days after birth, a decrease in the number of animals reactive to the MAT was detected. There was no significant statistical difference for the passive transfer of antibodies between single or twin lambs.

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Published

2020-08-13

How to Cite

CUCCATO, L.P., LIMA, A.M.C., DOS REIS, T.F.M., QUEIROZ, C.L., BUIATTE, A.B.G., CIUFFA, A.Z., GOMES, D.O., ALVES, V.B. de A. and ROSALINSKI-MORAES, F., 2020. Evaluation of response to anti-leptospira bacterin vaccination in pregnant ewes and the passive transfer of antibodies to their offspring. Bioscience Journal [online], vol. 36, no. 5, pp. 1715–1725. [Accessed6 December 2022]. DOI 10.14393/BJ-v36n5a2020-47708. Available from: https://seer.ufu.br/index.php/biosciencejournal/article/view/47708.

Issue

Section

Agricultural Sciences