Anti-inflammatory, antinociceptive and antioxidant activities of the hydromethanolic fraction from Annona nutans leaves

Authors

  • Nathália Lucca Silva Universidade Federal de São João Del-Rei
  • Aline Aparecida Saldanha Universidade Federal de São João Del-Rei
  • Denise Brentan Silva Universidade Federal do Mato Grossso do Sul
  • Carlos Alexandre Carollo Universidade Federal do Mato Grossso do Sul
  • Angela Lucia Bagnatori Sartori Universidade Federal do Mato Grossso do Sul
  • Adriana Cristina Soares Universidade Federal de São João Del-Rei
  • João Máximo de Siqueira Universidade Federal de São João Del-Rei

DOI:

https://doi.org/10.14393/BJ-v35n5a2019-45927

Keywords:

Annona nutans, Flavonoids, Anti-inflammatory, Antioxidant, Anti-nociceptive, HPLC quantification

Abstract

Annona nutans (Annonaceae) is a plant species found in Bolivia, Paraguay, Argentina, and the Brazilian Cerrado, specifically in the states of Mato Grosso and Mato Grosso do Sul (Brazil). Its common names are Araticû-Mi and Araticû-Ñu. The research contributions regarding the chemical composition and biological activities of extracts from A. nutans are rare, with only four articles being found in the literature. Therefore, the present study evaluated the anti-inflammatory and antinociceptive activities of the hydromethanolic fraction (FHMeOH) using carrageenan-induced paw edema and hot-plate tests. In addition, the antioxidant activity was evaluated by DPPH radical scavenging, total phenolic, flavonoid and tannin content assays and quantification of the major metabolites by LC-MS were performed. Oral treatment with the FHMeOH (at a dose of 300 mg.kg-1) significantly reduced paw edema 2 h and 4 h after the inflammatory stimulus. The intraperitoneal (i.p.) treatment with the FHMeOH (50 and 100 mg.kg-1) proved to be most effective, and the inhibition of acute inflammation was still visible 6 h after carrageenan injection. At doses of 50 and 100 mg.kg-1 (i.p.), FHMeOH exhibits central antinociceptive effects by increasing the latency of the reaction in the hot-plate model. The FHMeOH showed antioxidant potential, and the metabolites quercetin-3-O-galactoside, quercetin-3-O-glucoside, isorhamnetin-3-O-galactoside, quercetin-3-O-β-D-apiofuranosyl-(1→2)-galactopyranoside, and chlorogenic acid were identified and quantified by LC-MS. Our results indicate, for the first time, that FHMeOH has anti-inflammatory, antinociceptive and antioxidant potential, and it is a promising source of studies for new herbal medicines

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Published

2019-10-09

How to Cite

SILVA, N.L., SALDANHA, A.A., SILVA, D.B., CAROLLO, C.A., SARTORI, A.L.B., SOARES, A.C. and SIQUEIRA, J.M. de, 2019. Anti-inflammatory, antinociceptive and antioxidant activities of the hydromethanolic fraction from Annona nutans leaves. Bioscience Journal [online], vol. 35, no. 5, pp. 1599–1613. [Accessed2 December 2022]. DOI 10.14393/BJ-v35n5a2019-45927. Available from: https://seer.ufu.br/index.php/biosciencejournal/article/view/45927.

Issue

Section

Health Sciences