The chick embryo chorioallantoic membrane assay as a model for the study of angiogenesis


  • Angélida Daiane Lemos do Prado Universidade Estadual de Goiás
  • Elisa Flávia Luiz Cardoso Bailão Universidade Estadual de Goiás
  • João Carlos Nabout Universidade Estadual de Goiás
  • Tatiana Rabachini University of Bern
  • Paulo Roberto de Melo Reis Pontifícia Universidade Católica de Goiás
  • Pablo José Gonçalves Universidade Federal de Goiás
  • Luciane Madureira Almeida Universidade Estadual de Goiás



Alternative method, angiogenesis, bibliometric analysis, CAM assay, oncology, tumor vascularization.


Angiogenesis is a fundamental physiological process with strong implications in tissue homeostasis. Animal models helping to identify how angiogenesis is regulated are fundamental to answer many biological questions. Chick embryo chorioallantoic membrane (CAM) assay is one of the most employed methods to study angiogenesis. In this study we applied a scientometric approach to evaluate the employment of CAM assay in published articles. Temporal trends indicated that CAM assay was the preferred method to investigate angiogenesis over time. The publications had a significant number of citations and the impact factor of journals publishing articles is relevant for the scientific community. A total of 52 different research areas have articles published using this particular technique. Oncology is the research field in which CAM assay was mostly used. Accordingly, tumor-derived cell lines were the most frequent sample tested on CAM. We also identified that 73,6% of articles published used only CAM assay to answer questions concerning angiogenesis. We concluded that although the CAM assay is a classical approach, that does not need so much infrastructure and financial support to be performed, it is a well-accepted technique by the scientific community. In addition, this methodology has gain attention in scientific community because no pain is experienced by the chick and they are minor ethical concerns to employ this method. Moreover, this data can help researchers who are unfamiliar with the CAM assay to identify if this particular method is suitable for their research.



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How to Cite

DO PRADO, A.D.L., BAILÃO, E.F.L.C., NABOUT, J.C., RABACHINI, T., MELO REIS, P.R. de, GONÇALVES, P.J. and ALMEIDA, L.M., 2019. The chick embryo chorioallantoic membrane assay as a model for the study of angiogenesis. Bioscience Journal [online], vol. 35, no. 4, pp. 1262–1275. [Accessed24 February 2024]. DOI 10.14393/BJ-v35n4a2019-42777. Available from:



Health Sciences