A wide spectrum of antibacterial activity of secondary metabolites from Bacillus amyloliquefaciens ELI149

Authors

  • Estibaliz Sansinenea Benemérita Universidad Autónoma de Puebla
  • Jessica Vaca Benemérita Universidad Autónoma de Puebla
  • Norma Elena Rojas Benemérita Universidad Autónoma de Puebla
  • Candelario Vázquez Benemérita Universidad Autónoma de Puebla

DOI:

https://doi.org/10.14393/BJ-v35n6a2019-42660

Keywords:

Antibacterial, Amberlite, Bacillus amyloliquefaciens, secondary metabolites

Abstract

A highly potent secondary metabolites-producing Bacillus strain was isolated from Mexican soil (Puebla State), together with other fifty strains. The fifty-one strains were subjected for metabolites extraction and evaluated as antibacterial against several bacteria. The active metabolites of these strains were extracted using amberlite XAD16 absorbent resin. The antibacterial activity of crude extracts of all strains was performed by disk diffusion method against some pathogenic gram positive and gram-negative bacteria. Among all Bacillus strains tested, the most potent strain ELI149 (NRB) was selected for molecular characterization. The nucleotide sequence of the 16S rRNA gene (1.5 Kb) of this strain evidenced a 94% similarity with Bacillus amyloliquefaciens strain IIHR-Ba-2, which showed the highest inhibition against the most bacteria probed even greater inhibition than the standard antibiotic. In conclusion, secondary metabolites extracted from Bacillus amyloliquefaciens strain are highly potent as antibiotic against the most bacteria probed. Identification of which metabolites extracted from amberlite are the responsible of the bacteria growth inhibition will be a challenge.

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Published

2020-01-01

How to Cite

ESTIBALIZ SANSINENEA, VACA, J., ROJAS, N.E. and VÁZQUEZ, C., 2020. A wide spectrum of antibacterial activity of secondary metabolites from Bacillus amyloliquefaciens ELI149. Bioscience Journal [online], vol. 36, no. 1, pp. 235–244. [Accessed14 April 2024]. DOI 10.14393/BJ-v35n6a2019-42660. Available from: https://seer.ufu.br/index.php/biosciencejournal/article/view/42660.

Issue

Section

Biological Sciences