Acute toxicity of Jatropha curcas oil on plant cell cycle

Authors

  • Larissa Fonseca Andrade-Vieira Universidade Federal de Lavras
  • Carolina Mastella Botelho Universidade Federal do Espírito Santo
  • Bruno Galvêas Laviola Universidade Federal do Espírito Santo
  • Adésio Ferreira Universidade Federal do Espírito Santo
  • Milene Miranda Praça Fontes Universidade Federal do Espírito Santo

DOI:

https://doi.org/10.14393/BJ-v35n5a2019-42562

Keywords:

Aneugenic effects., Lactuca sativa., Mitotic index, Phorbol esters, Root growth.

Abstract

Today, a great interest in Jatropha-based products exists worldwide, mainly for the production of biofuel. However, the oil obtained from this plant is known to be toxic due to contained curcins and phorbol esters. Bioassays, including plant cytogenetic assays based on cell cycle observation, are useful for determining the toxicity of J. curcas oil. Hence, the aim of this study was to describe the mechanism of action of J. curcas oil by cell cycle analysis using Lactuca sativa as plant testing model. A decrease in root growth was observed, closely related to the reduction in mitotic index, along with an increase in condensed nuclei. J. curcas chemicals act both as aneugenic agents, leading to the formation of lagged, sticky chromosomes and c-metaphase cells, as well as clastogenic agents, inducing the formation of chromosome bridges and fragments. The cytotoxicity and genotoxicity of phorbol esters and other chemical components of J. curcas oil was determined and discussed.

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Published

2019-10-09

How to Cite

ANDRADE-VIEIRA, L.F.., BOTELHO, C.M.., LAVIOLA, B.G.., FERREIRA, A.. and FONTES, M.M.P., 2019. Acute toxicity of Jatropha curcas oil on plant cell cycle. Bioscience Journal [online], vol. 35, no. 5, pp. 1544–1551. [Accessed26 November 2022]. DOI 10.14393/BJ-v35n5a2019-42562. Available from: https://seer.ufu.br/index.php/biosciencejournal/article/view/42562.

Issue

Section

Biological Sciences