Preservation and maceration of amazon açai leaflet tissue to obtain genomic DNA

Authors

  • Hellen Sandra Freires da Silva Azêvedo Fundação Oswaldo Cruz (FIOCRUZ). http://orcid.org/0000-0003-1682-7232
  • Polinar Bandeira Rufino Faculdade Meta
  • José Marlo Araújo de Azevedo Instituto Federal do Acre
  • Luciélio Manoel da Silva Empresa Brasileira de Pesquisa Agropecuária
  • Lúcia Helena de Oliveira Wadt Empresa Brasileira de Pesquisa Agropecuária
  • Tatiana de Campos Empresa Brasileira de Pesquisa Agropecuária

DOI:

https://doi.org/10.14393/BJ-v35n4a2019-42190

Keywords:

Açaí-solteiro, CTAB method, DNA isolation, Euterpe precatoria

Abstract

The objective of this study was to test the efficiency of preservation and maceration methods for Euterpe precatoria leaflet tissue to obtain genomic DNA for molecular studies. The leaflets of E. precatoria were collected in an experimental field at Embrapa Acre, Brazil. The study was conducted in a completely randomized design with 10 replicates, in a 12 × 2 factorial structure, with 12 storage treatments (fresh; lyophiliser 3 days; refrigerator 3, 5, and 7 days; silica gel 7, 10, 20, and 30 days; and transport buffer 3, 5, and 7 days) and two leaf tissue maceration methods (liquid nitrogen and the TissueLyser®). Statistically significant differences in the obtained DNA concentration were found between the maceration and storage treatments. The TissueLyser® macerator produced higher DNA concentrations when compared to liquid nitrogen. For the storage treatments, five groups were formed based on DNA concentration when macerated with the TissueLyser® and two groups when macerated with liquid nitrogen. The DNA concentrations ranged from 285.00 ng/µL (7 days in transport buffer) to 702.00 ng/µL (30 days in silica gel) when the leaflets were macerated with liquid nitrogen, and from 572.73 ng/µL (30 days in silica gel) to 2,850.00 ng/µL (3 days in lyophiliser) using the TissueLyser® macerator. The DNA purity (A260/A280 nm) varied from 1.30 to 1.70 when the leaflets were macerated with liquid nitrogen and from 1.30 to 1.90 with the TissueLyser® macerator. Despite the variations in leaf tissue preservation and DNA concentration, all treatments were effective for DNA isolation and it was possible to amplify genomic regions of microsatellite markers by PCR. It was concluded that leaflets of E. precatoria stored in a lyophiliser and processed with an automatic macerator resulted in satisfactory DNA for molecular studies.

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Author Biography

Hellen Sandra Freires da Silva Azêvedo, Fundação Oswaldo Cruz (FIOCRUZ).

Doutoranda da Rede Bionorte.

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Published

2019-08-08

How to Cite

AZÊVEDO, H.S.F. da S., RUFINO, P.B., AZEVEDO, J.M.A. de, SILVA, L.M. da, WADT, L.H. de O. and CAMPOS, T. de, 2019. Preservation and maceration of amazon açai leaflet tissue to obtain genomic DNA. Bioscience Journal [online], vol. 35, no. 4, pp. 1188–1197. [Accessed5 December 2022]. DOI 10.14393/BJ-v35n4a2019-42190. Available from: https://seer.ufu.br/index.php/biosciencejournal/article/view/42190.

Issue

Section

Biological Sciences