Evaluation of Lysinibacillus SP, isolated from Coptotermes curvignathus Gut, for the delignification of oil palm residues

Authors

  • Fadilat Ayeronfe University Tun Hussein Onn Malaysia, Johor, Malaysia.
  • Angzzas Kassim University Tun Hussein Onn Malaysia,Johor, Malaysia.
  • Patricia Hung Universiti Putra Malaysia, Malaysia
  • Hafiz Zainulabidin University Tun Hussein Onn Malaysia, Johor, Malaysia.
  • Nadiah Ishak University Tun Hussein Onn Malaysia,Johor, Malaysia.
  • Sharfina Syarifah University Tun Hussein Onn Malaysia,Johor, Malaysia.
  • Ashuvila Aripin University Tun Hussein Onn Malaysia,Johor, Malaysia.

DOI:

https://doi.org/10.14393/BJ-v35n3a2019-42070

Keywords:

Biodelignification, Oil palm, Pulping, Lignin, Enzymes

Abstract

The application of ligninolytic bacteria and enzymes is a green pre-treatment alternative in the production of paper and biofuel from oil palm residues. In this study we investigated the ability of Lysinibacillus pakistanensis isolated from termite gut in degrading the lignin component of oil palm residues. The residues were biotreated with the bacterial strain in an aerated submerged fermentation system for 7 days at 30 , pH 7 and compared with untreated control. Enzyme activities were determined using specific substrates. Peak lignin peroxidase (377.6 U/L), manganese peroxidase (218.19 U/L), and laccase (405.4 U/L) activity were recorded after 4,4, and 5 days of incubation respectively, using oil palm leaf as substrates. Lignin loss of 4.5%, 5.7% and 6.6% in oil palm leaf, oil palm trunk and empty fruit bunch respectively was achieved after treatment with the microorganism. SEM images revealed structural changes in the cell wall of the residues. Pre-treatment with this bacterial strain has promising prospects of improving the efficiency of the pulping process in an environmentally safe manner.

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Published

2019-06-11

How to Cite

AYERONFE, F., KASSIM, A., HUNG, P., ZAINULABIDIN, H.., ISHAK, N., SYARIFAH, S. and ARIPIN, A., 2019. Evaluation of Lysinibacillus SP, isolated from Coptotermes curvignathus Gut, for the delignification of oil palm residues. Bioscience Journal [online], vol. 35, no. 3, pp. 892–902. [Accessed3 December 2022]. DOI 10.14393/BJ-v35n3a2019-42070. Available from: https://seer.ufu.br/index.php/biosciencejournal/article/view/42070.

Issue

Section

Agricultural Sciences