Process optimization for pectinase production by locally isolated fungal strain using submerged fermentation

Authors

  • Roheena Abdullah
  • Amna Jafer Department of Biotechnology Lahore college for Women university
  • Kinza Nisar Department of Biotechnology Lahore college for Women university
  • Afshan Kaleem Department of Biotechnology Lahore college for Women university
  • Mehwish Iqtedar Department of Biotechnology Lahore college for Women university
  • Tehreema Iftikhar Department of Botany Lahore College for women university Lahore
  • Faiza Saleem Department of Biotechnology Lahore college for Women university
  • Shagufta Naz Department of Biotechnology Lahore college for Women university

DOI:

https://doi.org/10.14393/BJ-v34n1a2018-39947

Keywords:

Pectinase, Aspergillus niger, Submerged fermentation, Wheat bran

Abstract

The present study deals with the isolation screening and optimization of fungal strain for pectinase production. The fungal strains were isolated from different sources, including soil, fruits etc. Qualitative screening was performed on the basis of the pectin hydrolysis zone. While, quantitative screening was carried out employing submerged fermentation. Among all the strains the strains showing highest pectinolytic potential were selected identified and assigned the code Aspergillus niger ABT-5.The influence of different fermentation media on pectinase production was evaluated. The M5 medium containing 10g wheat bran, nutrient medium containing (g/l) of (NH4)2SO4 6.0, K2HPO4 6.0, KH2PO4 6.0, MgSO4.7H2O 0.1 gave the highest pectinase production. The other important physico chemical parameters including incubation period, temperature, and volume of media, size of inoculum, carbon and nitrogen sources were also optimized for pectinase production. The highest pectinase production (15.5U/ml) was obtained at 72h of incubation, pH 6, temperature 30°C, volume of media 50ml. Fructose and urea were designated as best carbon and nitrogen sources subsequently.

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Published

2018-08-08

How to Cite

ABDULLAH, R., JAFER, A., NISAR, K., KALEEM, A., IQTEDAR, M., IFTIKHAR, T., SALEEM, F. and NAZ, S., 2018. Process optimization for pectinase production by locally isolated fungal strain using submerged fermentation . Bioscience Journal [online], vol. 34, no. 4, pp. 1025–1032. [Accessed28 November 2022]. DOI 10.14393/BJ-v34n1a2018-39947. Available from: https://seer.ufu.br/index.php/biosciencejournal/article/view/39947.

Issue

Section

Biological Sciences