Induction and growth curve of calli from leaf and nodal explants of genipap
Keywords:Genipa americana L., Callogenesis, Plant growth regulators
The aim of this study was to determine the effect of the auxin 2,4-D (2,4-dichlorophenoxyacetic) in calli formation from leaf and nodal segments of genipap and to characterize its growth curve. Explants obtained from shoots previously established from in vitro seedlings were used for calli induction. The experimental design was completely randomized in a 3x5x2 factorial with three accessions (NB, SA, SAL), five concentrations of 2,4-D (0.0; 2.0; 4.0, 6.0 or 8.0 mg L-1) and two times of measurement for calli fresh weight (30 and 60 days). There was callus formation in all treatments tested. It was observed that the best response for callus induction from leaf segments was with 2.0 mg L-1 of 2,4-D. For the nodal segment, the response among the accessions was different due to 2,4-D concentrations. The growth curve was plotted according to the fresh weight of callus obtained at intervals of 10 days up to 60 days. Through the established growth curve, the nodal-derived calli from accession SA should be transferred to a new medium, after 40 days of culture.
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Copyright (c) 2018 Annie Carolina Araújo de Oliveira, Caroline de Araújo Machado, Leila Albuquerque Resende de Oliveira, Francine Ferreira Padilha, Ana Veruska Cruz da Silva, Ana da Silva Lédo
This work is licensed under a Creative Commons Attribution 4.0 International License.