Induction and growth curve of calli from leaf and nodal explants of genipap

Authors

  • Annie Carolina Araújo de Oliveira Universidade Federal de Sergipe
  • Caroline de Araújo Machado Universidade Federal de Sergipe
  • Leila Albuquerque Resende de Oliveira Universidade Federal de Sergipe
  • Francine Ferreira Padilha Instituto de Tecnologia e Pesquisa
  • Ana Veruska Cruz da Silva Embrapa Tabuleiros Costeiros
  • Ana da Silva Lédo Embrapa Tabuleiros Costeiros

DOI:

https://doi.org/10.14393/BJ-v34n6a2018-39708

Keywords:

Genipa americana L., Callogenesis, Plant growth regulators

Abstract

The aim of this study was to determine the effect of the auxin 2,4-D (2,4-dichlorophenoxyacetic) in calli formation from leaf and nodal segments of genipap and to characterize its growth curve. Explants obtained from shoots previously established from in vitro seedlings were used for calli induction. The experimental design was completely randomized in a 3x5x2 factorial with three accessions (NB, SA, SAL), five concentrations of 2,4-D (0.0; 2.0; 4.0, 6.0 or 8.0 mg L-1) and two times of measurement for calli fresh weight (30 and 60 days). There was callus formation in all treatments tested. It was observed that the best response for callus induction from leaf segments was with 2.0 mg L-1 of 2,4-D. For the nodal segment, the response among the accessions was different due to 2,4-D concentrations. The growth curve was plotted according to the fresh weight of callus obtained at intervals of 10 days up to 60 days. Through the established growth curve, the nodal-derived calli from accession SA should be transferred to a new medium, after 40 days of culture.

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Published

2018-12-06

How to Cite

DE OLIVEIRA, A.C.A., MACHADO, C. de A., RESENDE DE OLIVEIRA, L.A., PADILHA, F.F., SILVA, A.V.C. da and LÉDO, A. da S., 2018. Induction and growth curve of calli from leaf and nodal explants of genipap . Bioscience Journal [online], vol. 34, no. 6, pp. 161–167. [Accessed23 May 2022]. DOI 10.14393/BJ-v34n6a2018-39708. Available from: https://seer.ufu.br/index.php/biosciencejournal/article/view/39708.

Issue

Section

Agricultural Sciences