Clonal cleaning and in vitro multiplication of Angelonia integerrima sprengel

Authors

  • Mara Cíntia Winhelmann Universidade Federal do Rio Grande do Sul
  • Marília Tedesco Universidade Federal do Rio Grande do Sul
  • Priscila Paris Universidade Federal do Rio Grande do Sul
  • Eduarda Demari Avrella Universidade Federal do Rio Grande do Sul
  • Claudimar Sidnei Fior Universidade Federal do Rio Grande do Sul
  • Gilmar Schafer Universidade Federal do Rio Grande do Sul

DOI:

https://doi.org/10.14393/BJ-v34n5a2018-39442

Keywords:

Pampa biome, stem apex, native ornamental, Plantaginaceae

Abstract

The objective of this study is to test stem apex sizes in the in vitro establishing of Angelonia integerrima in order to obtain explants without by fungi and bacteria contamination for further multiplications. The treatments consisted of different stem apex sizes (1.0, 3.0, 5.0, 7.0, 9.0 and 11.0 mm). At 45 and 90 days of cultivation, a count of contaminated explants and a count of shoots per explant formed were performed. In a second experiment, explants were cultivated in a medium containing different concentrations of benzylaminopurine (BAP) (0.0, 0.05, 0.10, 0.15 and 0.20 mg L-1). After 56 days of cultivation, the following variables were evaluated: shoot length, shoot fresh mass and number of shoots. During the explant establishment phase (45 days), only stem apexes with 1.0 mm in size were not contaminated. However, in the second subculture (at 90 days), only shoots from initial explants, with 7 mm in size or larger, were contaminated. Regarding multiplication, the presence of BAP showed a positive linear behavior for all variables. It is possible to obtain A. integerrima seedlings free of contamination in vitro by fungi and bacteria, using initial explants less than or equal to 5 mm. IBA provided a linear increment for the multiplication of this species.

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Published

2018-10-11

How to Cite

WINHELMANN, M.C., TEDESCO, M., PARIS, P., AVRELLA, E.D., FIOR, C.S. and SCHAFER, G., 2018. Clonal cleaning and in vitro multiplication of Angelonia integerrima sprengel . Bioscience Journal [online], vol. 34, no. 5, pp. 1258–1263. [Accessed5 December 2022]. DOI 10.14393/BJ-v34n5a2018-39442. Available from: https://seer.ufu.br/index.php/biosciencejournal/article/view/39442.

Issue

Section

Agricultural Sciences