Assessments on the catalytic and kinetic properties of beta-glucosidase isolated from a highly efficient antagonistic fungus Trichoderma harzianum

Authors

  • Elham Khalili Dept. of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, 81310 Skudai, Johor, Malaysia
  • Fahrul Huyop
  • Muhammad Arshad Javed
  • Naji Arafat Mahat
  • Kalaivani Batumalaie
  • Roswanira Abdul Wahab

DOI:

https://doi.org/10.14393/BJ-v34n1a2018-39384

Keywords:

β-glucosidase, Trichoderma harzianum, Kinetic assessment, Macrophomina phaseolina

Abstract

Due to the toxicity and inefficiency of chemical fungicides to control infestation of Macrophomina phaseolina (Tassi) Goid which causes charcoal rot in plants, a biotechnological approach using β-glucosidase (EC.3.2.1) as the alternative bioactive ingredient in fungicide is hereby, proposed. The extracellular enzyme was isolated from a highly efficient fungal antagonist, Trichoderma harzianum T12. The highly similar molecular masses obtained using SDS-PAGE (96 kDa) and MALDI-TOF mass spectrometry (98.3 kDa) affirmed that the β-glucosidase was purified to homogeneity. Consequently, optimum catalytic parameters that rendered the highest enzyme activity were found to be: 45˚C, pH 7, inoculum size of 10 % (w/v), supplementation with metal ions Zn2+ and Mn2+ ions, and Tween 80. Addition of wheat bran and (NH4)2SO4 as carbon and nitrogen sources also improved enzyme activity. BLASTn showed the sequence of β-glucosidase T12 was highly identical to other β-glucosidases viz. T. harzianum strain IOC-3844 (99%), T. gamsii and T. virens bgl1 (86 %) as well as T. reesei strain SJVTR and T. viride strain AS 3.3711 (84 %). Kinetic assessment showed that β-glucosidase T12 catalyzes hydrolytic activity is characterized by a Km of 0.79 mM and Vmax of 8.45 mM min-1 mg-1 protein, with a corresponding kcat of 10.69 s-1

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Author Biography

Elham Khalili, Dept. of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, 81310 Skudai, Johor, Malaysia

Dept of Chemistry and Senior lecturer

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Published

2018-08-08

How to Cite

KHALILI, E., HUYOP, F., JAVED, M.A., MAHAT, N.A., BATUMALAIE, K. and WAHAB, R.A., 2018. Assessments on the catalytic and kinetic properties of beta-glucosidase isolated from a highly efficient antagonistic fungus Trichoderma harzianum . Bioscience Journal [online], vol. 34, no. 4, pp. 830–847. [Accessed6 December 2022]. DOI 10.14393/BJ-v34n1a2018-39384. Available from: https://seer.ufu.br/index.php/biosciencejournal/article/view/39384.

Issue

Section

Agricultural Sciences