Industrial milk powder in bioassays for evaluation of cytotoxicity and genotoxicity


  • Rayssa Aléxia Martins Moura Universidade Federal do Piauí
  • Marcia Maria Mendes Marques Universidade Federal do Piauí
  • Helena Mendes Pereira Universidade Estadual do Ceará
  • Ila Monize Sousa Sales Universidade Federal do Piauí
  • Fabelina Karolyne Sousa Santos Universidade Federal do Piauí
  • Maria Izabel Florindo Guedes Universidade Federal do Piauí
  • Ana Paula Peron Universidade Federal do Piauí



powdered milk, cell division, mitotic spindle changes, cell viability, meristematic tissue, MTT test.


Considering the widespread consumption of milk powder by the general population as well as the lack of studies on the toxicity of such industrialized foods, this study evaluated the cytotoxic and genotoxic potential of powdered milk from four reputed companies in the food market of Brazil and other South American countries. Milk samples were evaluated in root meristem cells of Allium cepa L., at concentrations of 0.065 and 0.13 g/mL, for 24 and 48 hours of exposure; and by means of cell viability in culture of cells of normal lineage, via MTT test, for 24 hours, at concentrations of 0.016; 0.032; 0.065 and 0.13g/mL. The concentration 0.13 g/mL was the one suggested for consumption in all milk packages evaluated in this study. In A. cepa, we observed that the milks, at both concentrations and at the two exposure times investigated, reduced the cellular proliferation of root meristems demonstrating a significant cytotoxicity. Furthermore, 0.13g/mL milks at the exposure time of 24h induced an expressive frequency of cellular alterations in the plant tissue, showing to be genotoxic. In the in vitro evaluation, three milks at 0.065 g/mL and all milks at 0.13 g/mL have significantly reduced cell viability, proving to be cytotoxic to the analyzed cell culture. Therefore, under the studied conditions, the powdered milks evaluated caused significant genetic instability to the cells of the test systems used.


Download data is not yet available.




How to Cite

MOURA, R.A.M., MARQUES, M.M.M., PEREIRA, H.M., SALES, I.M.S., SANTOS, F.K.S., GUEDES, M.I.F. and PERON, A.P., 2017. Industrial milk powder in bioassays for evaluation of cytotoxicity and genotoxicity . Bioscience Journal [online], vol. 33, no. 6, pp. 1622–1631. [Accessed2 March 2024]. DOI 10.14393/BJ-v33n6a2017-37266. Available from:



Biological Sciences