Real-time PCR for traceability and quantification of genetically modified seeds in lots of non-transgenic soybean

Authors

  • Joelma Leão-Buchir Universidade Federal do Paraná
  • Gilberto Vinícius Melo Pereira Universidade Federal do Paraná
  • André Luís Lopes da Silva Universidade Federal do Paraná
  • Silvana Alban Universidade Federal do Paraná
  • Maria Carolina Rocha Universidade Federal do Paraná
  • Jossimara Polettini Universidade do Oeste Paulista
  • Vanete Thomaz-Soccol Universidade Federal do Paraná
  • Carlos Ricardo Soccol Universidade Federal do Paraná

DOI:

https://doi.org/10.14393/BJ-v34n1a2018-37236

Keywords:

Roundup Ready® soybean, GMP, Real Time PCR, Glycine max, cp4-epsps gene

Abstract

The constant presence of genetically modified (GM) soybean in conventional seed lots has become a growing problem for international seed trade. In this context, seed companies have prompted the development of routine tests for accurate genetically modified soybean seeds detection. In this study, a quantitative PCR-based method was standardized in order to detect and quantify mixtures of seeds (i.e. certified seed) or GM grains (i.e. seeds came from field) into samples of non-GM soybean, in a way that soybean lots can be assessed within the standards established by legislation. The method involved the use of p35S-f2/petu-r1 primers targeting CP-4 enolpyruvylshikimate-3-phosphate synthase (cp4-epsps) gene (i.e. that confers herbicide tolerance in Roundup ReadyTM (RR)) for real-time PCR detection and quantification through mericon Quant GMO Detection Assay. The results revealed the method efficiency to detect and quantify the presence of even one soybean seed in batch used for routine evaluation of GM seeds. In addition, it was possible to detect of up to 0.1% of transgenic DNA relative to the soybean grains content. Thus, the sensitive GMO quantitative approach described in this study will provide support in supervising activities, and facilitate the process and control of GM soybean.

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Author Biography

André Luís Lopes da Silva, Universidade Federal do Paraná

Possui graduação em Ciências Biológicas pela Universidade Federal de Santa Maria (2002), especialização em Biologia pela Universidade Federal de Santa Maria (2005) e em Biossegurança pela Universidade Federal de Santa Catarina (2005). Possui mestrado em Agronomia pela Universidade Federal de Santa Maria (2005). Atualmente cursa doutorado em Processos Biotecnológicos na Universidade Federal do Paraná (UFPR). Possui experiência em biologia vegetal com foco em Fisiologia, Genética e Biotecnologia, atuando principalmente nos temas: Cultura de Tecidos, Produção de Mudas, Biorreatores, Estresses abióticos, Transformação Genética com Plantas Ornamentais, Olericolas, Medicinais e Florestais. Revisor Científico dos periódicos Ciência Rural, Revista Brasileira de Biociências, Journal of Biotechnology and Biodiversity, Acta Scientiarum Biological Sciences e Journal of Developmental Biology and Tissue Engineering.

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Published

2017-12-20

How to Cite

LEÃO-BUCHIR, J., MELO PEREIRA, G.V., LOPES DA SILVA, A.L., ALBAN, S., ROCHA, M.C., POLETTINI, J., THOMAZ-SOCCOL, V. and SOCCOL, C.R., 2017. Real-time PCR for traceability and quantification of genetically modified seeds in lots of non-transgenic soybean . Bioscience Journal [online], vol. 34, no. 1, pp. 34–41. [Accessed2 December 2022]. DOI 10.14393/BJ-v34n1a2018-37236. Available from: https://seer.ufu.br/index.php/biosciencejournal/article/view/37236.

Issue

Section

Agricultural Sciences