Effect of different combinations of papain-based gels on dissolving pulp tissue

Authors

  • Matheus Melo Pithon Universidade Estadual do Sudoeste da Bahia
  • Rogério Lacerda-Santos Universidade Federal de Juiz de Fora
  • Gabriel Couto Oliveira Universidade Estadual do Sudoeste da Bahia
  • Caio Sousa Ferraz Universidade Estadual do Sudoeste da Bahia
  • Matheus Souza Campos Costa Universidade Estadual do Sudoeste da Bahia
  • Maíra Mercês Barreto Universidade Estadual do Sudoeste da Bahia
  • Daniel de Melo Silva Universidade Estadual Do Sudoeste Da Bahia

DOI:

https://doi.org/10.14393/BJ-v33n4a2017-37160

Keywords:

Papain, Dental pulp, Chlorhexidine, Dissolution

Abstract

The focus of this study was to evaluate the capacity to dissolve pulp tissue of various combinations of papain-based gels and other antimicrobial agents. 105 bovine pulps were used, of standardized sizes, fragmented into 15mm-sized portions and weighed on an analytical balance, divided into 7 groups (n=15): 1 - 0.9% Saline Solution (negative control); 2- 8% Papain gel; 3- 8% Papain gel + 0.5% Chloramine; 4- 0.5% Chloramine gel; 5- 8% Papain gel + 2% Chlorhexidine; 6- 2% Chlorhexidine gel; and 7- 5.25% Sodium Hypochlorite solution (positive control). After initial weighing, the pulp fragments were inserted in test tubes for dissolution for time intervals of 30, 60, 90 and 120 minutes, and then weighed again. The data were analyzed using the Kruskal-Wallis and Mann-Whitney tests (p<0.05). In the time interval of 120 minutes the 0.5% chloramine gel demonstrated 64.9% ability to pulp dissolve, followed by 8% papain gel with 61.3%; papain associated with 0.5% chloramine, 58%; and papain associated with 2% chlorhexidine, 55.4%; which showed statistically significant difference with 5.25% Sodium Hypochlorite (p<0.05). All the gels that contained papain and the 0.5% chloramine gel promoted pulp tissue dissolution, however on a significantly lower scale than 5.25% sodium hypochlorite. The 2% chlorhexidine demonstrated no capacity to dissolve pulp, as did the control.

Downloads

Download data is not yet available.

Author Biography

Rogério Lacerda-Santos, Universidade Federal de Juiz de Fora

Professor Adjunto de Ortodontia - UFJF

Doutor em Ortodontia - UFRJ

Downloads

Published

2017-07-25

How to Cite

PITHON, M.M., LACERDA-SANTOS, R., OLIVEIRA, G.C., FERRAZ, C.S., COSTA, M.S.C., BARRETO, M.M. and SILVA, D. de M., 2017. Effect of different combinations of papain-based gels on dissolving pulp tissue . Bioscience Journal [online], vol. 33, no. 4, pp. 1099–1105. [Accessed28 November 2022]. DOI 10.14393/BJ-v33n4a2017-37160. Available from: https://seer.ufu.br/index.php/biosciencejournal/article/view/37160.

Issue

Section

Health Sciences