Conventional and volatile salts as precipitating agents for recombinant antiviral protein

Authors

  • Kamilla Alves Carvalho Universidade Federal de Uberlândia
  • Dalton Giovanni Nogueira da Silva Universidade Federal de Uberlândia
  • Ronaldo Zucatelli Mendonça Universidade Federal de Uberlândia
  • Erika Ohta Watanabe Universidade Federal de Uberlândia
  • Fabiana Regina Xavier Batista Universidade Federal de Uberlândia

DOI:

https://doi.org/10.14393/BJ-v33n4a2017-36729

Keywords:

Measles, recAVLOEc, Salting out, Volatile salt

Abstract

Recombinant proteins expressed in cell culture have been shown to be relevant in the biopharmaceutical production focusing human health. The current work investigated the precipitation process of recAVLOEc protein, synthesized by E. coli BL21 (DE3) pLysS cells. The system is used for the AVLO expression that shown antiviral activity and it was found in the hemolymph of Lonomia obliqua caterpillar. The precipitation was conducted by the use of conventional salts (ammonium sulfate and sodium sulfate) and the volatile ammonium carbamate salt. Initially, the precipitated protein obtained from bacterial lysate was added to L929 cells to evaluate the cytotoxic effect; and besides Vero cells were infected with measles virus to verify the antiviral action of the precipitated recombinant protein. Toxic effect on the culture of L929 cells was observed for the precipitate obtained by the use of ammonium sulfate and sodium sulfate. In addition, tests in L929 cell cultures infected with EMC virus showed that samples of precipitated protein by salts did not show antiviral action. In Vero cell cultures, the precipitated protein by sodium sulfate showed antiviral action for measles virus.

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Author Biography

Fabiana Regina Xavier Batista, Universidade Federal de Uberlândia

Núcleo de Processos Biotecnológicos / Biotecnologia

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Published

2017-07-25

How to Cite

CARVALHO, K.A., NOGUEIRA DA SILVA, D.G., MENDONÇA, R.Z., WATANABE, E.O. and XAVIER BATISTA, F.R., 2017. Conventional and volatile salts as precipitating agents for recombinant antiviral protein . Bioscience Journal [online], vol. 33, no. 4, pp. 1048–1053. [Accessed7 December 2022]. DOI 10.14393/BJ-v33n4a2017-36729. Available from: https://seer.ufu.br/index.php/biosciencejournal/article/view/36729.

Issue

Section

Health Sciences