In vitro conservation and leaf anatomy of different chemotypes of Lippia alba (Mill.) N. E. BR


  • Magna Galvão Peixoto Universidade Federal de Sergipe
  • Ana Catarina Lima de Oliveira Instituto Federal de Sergipe
  • Arie Fitzgerald Blank Universidade Federal de Sergipe
  • Jefferson Henrique Santos Silva Universidade Federal de Sergipe
  • José Magno Queiroz Luz Universidade Federal de Uberlândia
  • Maria de Fátima Arrigoni-Blank Universidade Federal de Sergipe



Verbenaceae, Slow Growth, Plant anatomy, Morphology


The aim of this work was to develop an in vitro conservation protocol for Lippia alba that involved mineral oil and to study the leaf anatomy of L. alba genotypes in in vitro and ex vitro environments. In vitro conservation involved five genotypes; LA-13 and LA-57 (carvone chemotype); LA-22 (linalool chemotype); and LA-29 and LA-44 (citral chemotype). Each genotype was treated with mineral oil, with water, and a control at 18°C and 23°C. Survival (%), shoot height (cm) and leaf color were assessed at 180 and 270 days. The genotypes with the best in vitro conservation outcomes (LA-13 and LA-57) were used to study the anatomy of the leaves. Midrib thickness, leaf blade thickness, adaxial and abaxial cuticles and the number of glandular and tector trichomes were assessed. A temperature of 18°C was optimal for the conservation of genotypes of the carvone chemotypes. The explants were short and had green leaves. This was especially true for LA-57, whose height did not exceed 2.0 cm. At 270 days, the LA-13 and LA-57 genotypes continued to have green and viable leaves, especially LA-57, which had the lowest mean height. Field plants of the carvone chemotype had thicker midribs, leaf blades, and cuticles and had more trichomes. The LA-57 genotype had the most glandular and tector trichomes. 


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Author Biography

Arie Fitzgerald Blank, Universidade Federal de Sergipe

Professor, Doutor, Departamento de Engenharia Agronômica




How to Cite

PEIXOTO, M.G., OLIVEIRA, A.C.L. de, BLANK, A.F., SILVA, J.H.S., LUZ, J.M.Q. and ARRIGONI-BLANK, M. de F., 2017. In vitro conservation and leaf anatomy of different chemotypes of Lippia alba (Mill.) N. E. BR . Bioscience Journal [online], vol. 33, no. 1, pp. 41–51. [Accessed19 May 2024]. DOI 10.14393/BJ-v33n1a2017-35757. Available from:



Agricultural Sciences