Determination and validation of secnidazole in tablets by UV spectrophotometric

Authors

  • Márcia Regina Marcílio Universidade Federal de Mato Grosso
  • Alexia Lorenzi Raiser Universidade Federal de Mato Grosso
  • Ligia Paula Fumagalli Universidade Federal de Mato Grosso
  • Rudy Bonfilio Universidade Federal de Alfenas
  • Carla Regina Andrighetti Universidade Federal de Mato Grosso
  • Elton Brito Ribeiro Universidade Federal de Mato Grosso
  • Denia Mendes de Sousa Valladão Universidade Federal de Mato Grosso

DOI:

https://doi.org/10.14393/BJ-v33n5a2017-34889

Keywords:

Quality control, Quantify, Dissolution test, Secnidazole

Abstract

Secnidazole, a 5-nitroimidazole, is a drug used in the treatment against protozoa, and several bacterial infections. This study purpose was to develop and validate a UV spectrophotometric method to determine secnidazole in pharmaceutical tablet dosage forms once there is no method reported in the pharmacopoeia yet. The quantification was performed using methanol as solvent at 325 nm (maximum wavelength) and three kinds of products marketed in Brazil (reference, generic and similar tablets) containing 1g of secnidazole. The method obeyed Beer's law in the concentration range of 4 - 20 mgmL-1 respectively. The method was validated according to the International Conference on Harmonization (ICH) and Brazil National Health Surveillance Agency (ANVISA) guidelines, showing accuracy, precision, selectivity, robustness and linearity. Tests such as weight range, friability, disintegration, hardness and dissolution were carried out to check tablets' quality and all the trials showed to be in accordance with the general test guidelines of the Brazilian Pharmacopoeia. The dissolution test was carried out and the developed method was applied. The method developed is suitable for the estimation of secnidazole in tablets without any interference from the excipients and can be used for routine in quality control. Still, it's a simple, fast and low cost method.

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Published

2017-09-21

How to Cite

MARCÍLIO, M.R., RAISER, A.L., FUMAGALLI, L.P., BONFILIO, R., ANDRIGHETTI, C.R., RIBEIRO, E.B. and VALLADÃO, D.M. de S., 2017. Determination and validation of secnidazole in tablets by UV spectrophotometric . Bioscience Journal [online], vol. 33, no. 5, pp. 1351–1361. [Accessed28 May 2022]. DOI 10.14393/BJ-v33n5a2017-34889. Available from: https://seer.ufu.br/index.php/biosciencejournal/article/view/34889.

Issue

Section

Health Sciences