Antimicrobial activity of endophytic fungi from coffee plants

Authors

  • Mônica Cristina Pereira Monteiro Universidade Federal de Lavras - UFLA
  • Natálie Martins Alves Universidade Federal de Lavras - UFLA
  • Marisa Vieira de Queiroz Universidade Federal de Viçosa- UFV
  • Danilo Batista Pinho Universidade de Brasília
  • Olinto Liparini Pereira Universidade Federal de Viçosa -UFV
  • Sara Maria Chalfoun de Souza Empresa de Pesquisa Agropecuária de Minas Gerais - EPAMIG
  • Patrícia Gomes Cardoso Universidade Federal de Lavras - UFLA

DOI:

https://doi.org/10.14393/BJ-v33n2-34494

Keywords:

Aspergillus ochraceus, Fusarium verticillioides, Inhibition, Muscodor spp., Volatiles compounds.

Abstract

Endophytic fungi are a promising source for discovery of compounds with biotechnological potential. The aim of this study was to select and identify endophytic fungi from Coffea arabica that produce volatile organic compounds (VOCs), evaluate the effect of the VOCs produced by endophytic fungi on the growth of Rhizoctonia solani, Fusarium oxysporum, Phoma sp., Botrytis cinerea, Fusarium solani, Fusarium verticillioides, Cercospora coffeicola and Pestalotia longisetula, and select endophytic fungi with potential for biological control of Aspergillus ochraceus inoculated in coffee beans and F. verticillioides inoculated in corn seeds. An isolate of Muscodor albus was used as selection tool for VOC producing fungi. Among the 400 endophytic fungi isolates, 11 were able to grow in the presence of VOCs produced by M. albus. These fungi were identified as Muscodor spp. (9) and Simplicillium sp. according to searches in UNITE database using DNA sequences of internal transcribed spacer (ITS). The VOC's produced by endophytic fungi inhibited the growth the phytopathogenic fungi with different efficacies, compared to the control. The VOCs produced by Muscodor coffeanum (COAD 1842) showed fungicidal effect against A. ochraceus on coffee beans. Six endophytic fungi completely inhibited growth of F. verticillioides inoculated in corn seeds. This study demonstrates that the volatile-compound producing endophytic fungi, isolated from Coffea arabica, are promising sources of bioactive compounds.

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Author Biographies

Mônica Cristina Pereira Monteiro, Universidade Federal de Lavras - UFLA

Departamento de Ciências Biológicas/DBI - Lavras

Natálie Martins Alves, Universidade Federal de Lavras - UFLA

Departamento de Ciências Biológicas/DBI - Lavras

Marisa Vieira de Queiroz, Universidade Federal de Viçosa- UFV

Departamento de Microbiologia Agrícola

Danilo Batista Pinho, Universidade de Brasília

Departamento de Fitopatologia/DFP - Brasília -DF

Olinto Liparini Pereira, Universidade Federal de Viçosa -UFV

Departamento de Fitopatologia/DFP - Viçosa- MG.

Sara Maria Chalfoun de Souza, Empresa de Pesquisa Agropecuária de Minas Gerais - EPAMIG

Centro Tecnológico do Sul de Minas, Campus da UFLA -  Lavras

Patrícia Gomes Cardoso, Universidade Federal de Lavras - UFLA

Departamento de Ciências Biológicas/DBI

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Published

2017-03-30

How to Cite

MONTEIRO, M.C.P., ALVES, N.M., DE QUEIROZ, M.V., PINHO, D.B., PEREIRA, O.L., DE SOUZA, S.M.C. and CARDOSO, P.G., 2017. Antimicrobial activity of endophytic fungi from coffee plants . Bioscience Journal [online], vol. 33, no. 2. [Accessed29 November 2022]. DOI 10.14393/BJ-v33n2-34494. Available from: https://seer.ufu.br/index.php/biosciencejournal/article/view/34494.

Issue

Section

Biological Sciences