Potent anti-oxidant and anti-inflammatory potentials of Punica granatum leaf and flower hydromethanolic extracts in vitro


  • Ibtsam Gheith 1Department of Medical Laboratories Technology, Faculty of Applied Medical Sciences, Taibah University, 344 Medinah, Kingdom of Saudi Arabia. 2Department of Biotechnology, Animal Health Research Institute, 11843 Dokki, Egypt.
  • Abubakr El-Mahmoudy 3Department of Pharmacology, Faculty of Veterinary Medicine, Benha University. 13736 Moshtohor, Qalioubeya, EGYPT.




Punica granatum, Extract, Antioxidant, Anti-inflammatory, Oxidative stress.


Punica granatum flower and leaf were extracted with hydromethanol 50% and evaluated for antioxidant activity using phosophomolybdenum, DPPH, reducing power, and -OH-scavenging assays; and for anti-inflammatory activity using HRBC-MS assay. Both extracts exhibited antioxidant activities indicated by DPPH decolorization and -OH-scavenging and increased Ferric reducing and phosphomolybdenum assays. Also, both extracts showed anti-inflammatory activities indicated by red blood cell membrane stabilization and reduced haemolysis-resultant haemoglobin. All results were concentration-dependent; and the leaf extract was more potent than that of the flower. The IC50 values for the flower and leaf extracts were 777.6 ± 1.48 & 656.4 ± 1.79; 184.3 ± 1.803 & 113.9 ± 2.001; 130.8 ± 1.66 & 81.4 ± 2.1; 132.4 ± 1.55 & 79.67 ± 0.03; and 126.1 ± 1.35 & 67.25 ± 1.28 μg/mL in phosophomolybdenum, DPPH, reducing power, -OH-scavenging and red blood cell-membrane stability assays, respectively. Phytochemical analysis revealed presence of tannins and flavonoids with results more obvious in the leaf extract. These active principle contents may account for the recorded antioxidant and anti-inflammatory activities of both extracts. These findings provide evidence for the possible beneficial applications of the Punica granatum leaf and flower extracts in stress-related disease conditions and for maintenance of normal health status and well-being as well.


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How to Cite

GHEITH, I. and EL-MAHMOUDY, A., 2017. Potent anti-oxidant and anti-inflammatory potentials of Punica granatum leaf and flower hydromethanolic extracts in vitro . Bioscience Journal [online], vol. 33, no. 2. [Accessed23 February 2024]. DOI 10.14393/BJ-v33n2-33736. Available from: https://seer.ufu.br/index.php/biosciencejournal/article/view/33736.



Biological Sciences