Influence of dexamethasone and levamisole on macrophage recruitment, giant cell formation and blood parameters in the tropical fish Piaractus mesopotamicus

Authors

  • Thalita R. Petrillo Universidade Estadual Paulista
  • Gustavo da Silva Claudiano Universidade Estadual Paulista
  • Jefferson Yunis-Aguinaga UNESP http://orcid.org/0000-0002-1959-4350
  • Wilson Gomez Manrique Universidade Estadual Paulista
  • Marcelo Pardi de Castro Universidade Estadual Paulista
  • Marco Antonio de Andrade Belo Universidade Estadual Paulista
  • Julieta R. Engrácia de Moraes Universidade Estadual Paulista
  • Flávio Ruas de Moraes Universidade Estadual Paulista

DOI:

https://doi.org/10.14393/BJ-v33n4a2017-33016

Keywords:

Glucocorticoid, Coverslips, Inflammation, Pacu

Abstract

The aim of this study was to evaluate the effect of parenteral administration of dexamethasone and levamisole on the accumulation of macrophages and formation of giant cells in chronic inflammation by foreign body and blood parameters in pacu (Piaractus mesopotamicus). It was used 50 mg/kg of levamisole and 2.0 mg/kg of dexamethasone and the combination of both drugs. Coverslips were implanted under the skin. After 2, 7, and 15 days post-implantation (DPI), fish were anesthetized for removal of coverslips and the number of macrophages and giant cells were count. It was also collected blood from the caudal vessel to evaluate: red blood cell count, hematocrit, hemoglobin concentration, leukocytes count, thrombocytes count, MCV, and MCHC. We observed that dexamethasone affects negatively the formation of giant cells in the chronic inflammation for foreign body. Levamisole, despite being immunostimulatory in several species, showed limited action. However, it was enough to counteract the effect of dexamethasone; the association of the drugs did not interfere significantly in erythrocyte and leukocyte number in most of the treatments and times studied. In dexamethasone group there was a reduction in the number of erythrocytes and hemoglobin associated with increased mean corpuscular volume, suggesting slight macrocytic anemia. At 15 DPI, most groups showed the recovery of hematologic response. As in mammals, dexamethasone affects negatively the inflammatory response. Levamisole showed little effect by itself. However, in some parameters the association of both drugs causes similar response to control and naïve groups, showing the antagonistic effect of these drugs.

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Published

2017-07-25

How to Cite

PETRILLO, T.R., CLAUDIANO, G. da S., YUNIS-AGUINAGA, J., GOMEZ MANRIQUE, W., DE CASTRO, M.P., BELO, M.A. de A., DE MORAES, J.R.E. and DE MORAES, F.R., 2017. Influence of dexamethasone and levamisole on macrophage recruitment, giant cell formation and blood parameters in the tropical fish Piaractus mesopotamicus . Bioscience Journal [online], vol. 33, no. 4, pp. 1015–1027. [Accessed22 May 2022]. DOI 10.14393/BJ-v33n4a2017-33016. Available from: https://seer.ufu.br/index.php/biosciencejournal/article/view/33016.

Issue

Section

Agricultural Sciences