Production of xylanase by a new strain of Thermoascus aurantiacus: obtainment of enzymatic extract with reduced cellulolytic activity for application in pulp and paper industries

Authors

  • Ana Carolina da Costa Universidade Federal da Grande Dourados
  • Rodrigo Prudente Scalabrini Universidade Federal da Grande Dourados
  • Maria Alice Silvestre Universidade Federal da Grande Dourados
  • André Rodrigues Universidade Federal da Grande Dourados
  • Marcelo Fossa da Paz Universidade Federal da Grande Dourados
  • Gustavo Graciano Fonseca Universidade Federal da Grande Dourados

DOI:

https://doi.org/10.14393/BJ-v32n4a2016-32992

Keywords:

Solid State Fermentation, Pulp and paper bleaching, Cellulases and hemicellulases

Abstract

Xylanases are useful in several industrial segments, including pulp and paper bleaching, animal feed, and bread-making processes. However, the industrial use of these enzymes is closely related to its production cost and its catalytic properties. The process of solid state fermentation enables the use of agro-industrial residues as substrates for microbial cultivation and enzymes production, reducing costs. In the present study, different cultivation parameters were evaluated for the xylanase production by the thermophilic fungus Thermoascus aurantiacus, by solid state fermentation, using agro-industrial residues as substrates. High production of xylanase (1701.9 U g-1 of dry substrate) was obtained using wheat bran containing 65% of initial moisture, at 120 h of cultivation, and 45°C. The xylanase showed optimal activity at pH 5.0 and 75°C; its stability was maintained at pH 3.0-11.0. The enzyme retained its catalytic potential after 1 h, at 75°C. The enzymatic extract produced under optimized conditions showed reduced activities of endoglucanase and FPase. Our results, including the xylanase production by T. aurantiacus in low-cost cultivation medium, high structural stability of the enzyme, and reduced cellulolytic activity, encourage the application of this enzymatic extract in pulp and paper bleaching processes. 

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Published

2016-07-08

How to Cite

COSTA, A.C. da, SCALABRINI, R.P., SILVESTRE, M.A., RODRIGUES, A., PAZ, M.F. da and FONSECA, G.G., 2016. Production of xylanase by a new strain of Thermoascus aurantiacus: obtainment of enzymatic extract with reduced cellulolytic activity for application in pulp and paper industries . Bioscience Journal [online], vol. 32, no. 4, pp. 1040–1048. [Accessed28 November 2022]. DOI 10.14393/BJ-v32n4a2016-32992. Available from: https://seer.ufu.br/index.php/biosciencejournal/article/view/32992.

Issue

Section

Biological Sciences