Assessment of carcinogenic potential of soft drinks of cola, diet cola, orange and lemon, produced in the city of Uberlândia, Minas Gerais state, Brazil


  • Cássio Resende de Morais Universidade Federal de Uberlândia
  • Thays Cunha Vieira Fundação Carmelitana Mário Palmério
  • Renato Mendonça Borges Universidade Federal de Uberlândia
  • Luisa Mariana Melo Guimarães Universidade Federal de Uberlândia
  • Lucas Almeida Barcelos Universidade Federal de Uberlândia
  • Fernanda Carvalho Souza Universidade Federal de Uberlândia
  • Leticia Santos Pimentel Universidade Federal de Uberlândia
  • Jéssica Costa da Silva Universidade Federal de Pernambuco
  • Mirley Alves Vasconcelos Universidade Federal de Uberlândia
  • Tamiris Sabrina Rodrigues Universidade Federal de Uberlândia
  • Francielle Aparecida de Souza Universidade de Uberaba
  • Alexandre Azenha Alves de Rezende Universidade federal de Uberlândia
  • Mário Antônio Spanó Universidade Federal de Uberlândia
  • Ana Maria Bonetti Universidade Federal de Uberlândia



Soft drinks, wts, Drosophila melanogaster


Soft drinks are industrialized unfermented beverages, free of alcohol, carbonated, rich in artificial flavors and sugar. The intense consumption of such beverages can be related to not inheritable diseases such as caries, allergy, cellulite and stretch marks, gastrointestinal disorders, diabetes and cancer. The aim of this study was to evaluate the carcinogenic potential of different concentrations of soft drinks produced in the Uberlândia city, Minas Gerais State, Brazil, by means of Epithelial Tumor Detection Test using Drosophila melanogaster as a model. Third stage larvae descendants of crosses between D. melanogaster virgin females wts/TM3, sb¹ and males mwh/mwh were treated with different concentrations (0.83, 1.66 or 3.33 mL/g) of cola, diet cola, orange or lemon soft drinks. The total epithelial tumor rate observed in flies treated with 3.33 mL/g of cola and orange soft drinks was higher than the negative control. The diet cola and lemon caused no significant increase in the overall frequency of epithelial tumors in D. melanogaster. In conclusion, in these experimental conditions, the cola and orange base soft drinks demonstrated carcinogenic potential in somatic cells of D. melanogaster in the concentration of 3.33 mL/g.


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Author Biographies

Cássio Resende de Morais, Universidade Federal de Uberlândia

Master in Genetics and Biochemistry

Alexandre Azenha Alves de Rezende, Universidade federal de Uberlândia

PhD in Genetics and Biochemistry




How to Cite

DE MORAIS, C.R., VIEIRA, T.C., BORGES, R.M., GUIMARÃES, L.M.M., BARCELOS, L.A., SOUZA, F.C., PIMENTEL, L.S., SILVA, J.C. da, VASCONCELOS, M.A., RODRIGUES, T.S., SOUZA, F.A. de, REZENDE, A.A.A. de, SPANÓ, M.A. and BONETTI, A.M., 2016. Assessment of carcinogenic potential of soft drinks of cola, diet cola, orange and lemon, produced in the city of Uberlândia, Minas Gerais state, Brazil . Bioscience Journal [online], vol. 32, no. 4, pp. 1025–1039. [Accessed27 May 2024]. DOI 10.14393/BJ-v32n4a2016-32969. Available from:



Biological Sciences