Efficacy of Trichoderma asperellum, T. harzianum, T. longibrachiatum and T. reesei against Sclerotium rolfsii

Authors

  • Klênia Rodrigues Pacheco Universidade de Brasília
  • Bernardo Souza Mello Viscardi Universidade de Brasília
  • Thais Melissa Macedo de Vasconcelos Universidade de Brasília
  • Geisianny Augusta Monteiro Moreira Universidade de Brasília
  • Helson Mario Martins do Vale Universidade de Brasília
  • Luiz Eduardo Bassay Blum Universidade de Brasília http://orcid.org/0000-0002-3968-6615

DOI:

https://doi.org/10.14393/BJ-v32n2a2016-32732

Keywords:

Aethelia rolfsii, Phaseolus vulgaris, Biocontrol, Trichoderma spp.

Abstract

This work was carried out to select and to evaluate efficacy of Trichoderma isolates to control sclerotium wilt (Sclerotium rolfsii) of common-bean (Phaseolus vulgaris). For the experiments were used two isolates of S. rolfsii UB 193 and UB 228. Sixty-five Trichoderma spp. isolates were tested and the following ones were selected in vitro for the in vivo tests: 5, 11, 12, 15, 102, 103, 127, 136, 137, 1525 (T. longibrachiatum), 1637 (T. reesei), 1642, 1643 (T. harzianum), 1649 (T. harzianum), 1700 (T. asperellum) and EST 5. The most promising isolates were identified by Sequencing of the internal transcribed spacer regions ITS1, ITS2, and the 5.8s rRNA genomic region, using the ITS5 and ITS4 primers and compared with sequences in the National Center for Biotechnology Information (NCBI) database. These selected isolates 1649 (T. harzianum), 1525 (T. longibrachiatum) and 1637 (T. reesei) were tested for evaluation of sclerotial germination inhibition under laboratory conditions, and to evaluate the effects of these on disease of bean plants under greenhouse conditions. The Trichoderma isolates 1649, 1525 and 1637 were more efficient in reducing sclerotial germination. In addition to 1649, 1525 and 1637, the isolates 5, 12, 102 and 1525 (T. longibrachiatum) significantly reduced de amount of diseased bean plants under greenhouse conditions. 

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Author Biography

Luiz Eduardo Bassay Blum, Universidade de Brasília

Departamento de Fitopatologia

Professor

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Published

2016-04-04

How to Cite

PACHECO, K.R., VISCARDI, B.S.M., VASCONCELOS, T.M.M. de, MOREIRA, G.A.M., VALE, H.M.M. do and BLUM, L.E.B., 2016. Efficacy of Trichoderma asperellum, T. harzianum, T. longibrachiatum and T. reesei against Sclerotium rolfsii . Bioscience Journal [online], vol. 32, no. 2, pp. 412–421. [Accessed28 November 2022]. DOI 10.14393/BJ-v32n2a2016-32732. Available from: https://seer.ufu.br/index.php/biosciencejournal/article/view/32732.

Issue

Section

Agricultural Sciences