Action of leucine in heart hypertrophy induced by thyroid hormone in rats


  • Thiago Montes Fidale Universidade Federal de Uberlândia
  • Alexandre Gonçalves Universidade Federal de Uberlândia
  • Eduardo Gaspareto Haddad Universidade Federal de Uberlândia
  • Fernanda Rodrigues de Souza Universidade Federal de Uberlândia
  • Beatriz Montes Fidale Universidade Federal de Uberlândia
  • Fernando Cézar Veloso Universidade Federal de Uberlândia
  • Elmiro Santos Resende Universidade Federal de Uberlândia



Hyperthyroidism, BCAA, cardiac remodeling


Leucine is a regulator of protein metabolism in vivo, but there is little information regarding its effect on hyperthyroidism-induced cardiac hypertrophy. This study aimed to verify the action of leucine in heart hypertrophy using an experimental model of hyperthyroidism in rats. A total of 40 Wistar rats were used and allocated randomly into four groups: control (C), hormone (H), leucine (L) and hormone plus leucine (HL). Hyperthyroidism was induced by administering daily by gavage 20 μg of levothyroxine sodium in aqueous suspension per 100 g of body weight, and leucine was supplemented by adding 5% of the amino acid to conventional food. After 28 days, the heart was removed and weighed; the left ventricle was separated along with the interventricular septum and weighed. Histological slides were made from the left ventricle and stained with hematoxylin-eosin to measure the transverse diameter of cardiomyocytes. Two-way analysis of variance (ANOVA) and Tukey post-test were used and significance level set at 5%. There was a significant increase in the absolute heart weight, the left ventricular weight and cardiomyocyte diameter in rats of the H group compared to the other groups. The addition of leucine inhibited heart hypertrophy. Levothyroxine sodium-induced cardiac hypertrophy in Wistar rats is inhibited by leucine.


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How to Cite

FIDALE, T.M., GONÇALVES, A., HADDAD, E.G., DE SOUZA, F.R., FIDALE, B.M., VELOSO, F.C. and RESENDE, E.S., 2016. Action of leucine in heart hypertrophy induced by thyroid hormone in rats . Bioscience Journal [online], vol. 32, no. 4, pp. 1080–1084. [Accessed19 May 2024]. DOI 10.14393/BJ-v32n4a2016-32378. Available from:



Health Sciences