Assessment of intracanal medications cytotoxicity on L929 fibroblast cells


  • Michelle de Paula Farias Universidade Federal de Sergipe
  • Felipe de Souza Matos Universidade Federal de Sergipe
  • Nayane Chagas Carvalho Universidade Federal de Sergipe
  • Roque Pacheco de Almeida Universidade Federal de Sergipe
  • Adriano Augusto Melo de Mendonça Universidade Federal de Sergipe
  • Ricardo Luiz Cavalcanti de Albuquerque Júnior Centro Universitário Tiradentes
  • Maria Amália Gonzaga Ribeiro Universidade Federal de Sergipe



Cytotoxicity tests, immunologic, Endodontics, Calcium hydroxide, Iodoform, Fibroblasts


This study aimed to evaluate the cytotoxicity of intracanal medications on L929 fibroblast cells at different periods of observation. The following experimental groups were studied: calcium hydroxide with camphorated paramonochlorophenol and glycerin (CPG); iodoform with glycerin (IG); calcium hydroxide with iodoform and distilled water (CIW); iodoform with distilled water (IW); calcium hydroxide with distilled water (CW); Otosporin® (OT); and a control group composed of cells and culture medium. Eluates were prepared from each group and placed in contact with 1 x 105 cells/well for periods of 30 minutes, 12, 24, 48 and 72 hours, 5 and 7 days. After each experimental period, a cytotoxicity test was performed using methyltetrazolium (MTT) and a spectrophotometer at an optical density of 570 nm to analyze cell viability. The ANOVA and Tukey test with a significance level of 5% was used to analyze the data. At 30 minutes and at 12 hours, all groups were equal to the control group. At 24 hours, there was greater cytotoxicity in the IG group than in the control group (P<0.001). At 48 hours, only the OT group was cytotoxic (P <0.001). At 72 hours and at 5 days, the most cytotoxic groups were CW and OT. At 7 days, the IW and CPG groups were the least cytotoxic (P <0.001). With respect to experimental time, significant differences between 24 hours and 7 days were observed in all groups. Otosporin® was the most cytotoxic medication, followed by calcium hydroxide with distilled water.


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How to Cite

FARIAS, M. de P., MATOS, F. de S., CARVALHO, N.C., DE ALMEIDA, R.P., DE MENDONÇA, A.A.M., DE ALBUQUERQUE JÚNIOR, R.L.C. and RIBEIRO, M.A.G., 2016. Assessment of intracanal medications cytotoxicity on L929 fibroblast cells . Bioscience Journal [online], vol. 32, no. 2, pp. 566–573. [Accessed28 May 2024]. DOI 10.14393/BJ-v32n2a2016-30974. Available from:



Health Sciences