Experimental study on hplc determination of astragaloside in qishen huoxue granule and its protective effect in septic rats

Authors

  • Xiaoqi Sun Changchun Medical College
  • Hong Zhang Changchun Medical College
  • Wenhua Zou Changchun Medical College
  • Ying Chen ICU of The First Hospital of Jilin University

DOI:

https://doi.org/10.14393/BJ-v32n1a2016-29581

Keywords:

Astragaloside, HPLC, Qishen Huoxue Granule

Abstract

Objective: To determine the astragaloside content in Qishen Huoxue Granule (QHG) by HPLC and to investigate the protective effect of astragaloside in septic rats. Methods: Dikma DiamonsilTM C18 column was used. Mobile phase was methanol: acetonitrile (42:58) and detection was performed at 205 nm. Healthy rats were randomly divided into three groups, group A: sham operation (SO) group; group B: cecal ligation and puncture (CLP) group; and group C: CLP QHG treatment group. 72 h after model establishment, plasma tumor necrosis factor-α (TNF-α), plasma and peritoneal albumin concentrations, as well as mesenteric venular flow velocity in each group were measured and degree of intestinal mucosal injury was observed. Results: Average recovery was 100.7%, with a RSD of 2.20%. Sample concentration exhibited good linearity with peak area within a 0.01~0.2 mg/mL range. After CLP, plasma TNF-α concentration and albumin leakage in rats increased markedly; such increases were more significant in group B than group C (P<0.01). Changes in intestinal mucosal scores also presented the same trend (P<0.01). Mesenteric venular flow slowed down and plasma albumin concentration lowered; such decreases were more significant in group B than group C (P<0.05). Conclusion: QHG can reduce the inflammatory response and intestinal mucosal injury in rats with CLP-induced sepsis.

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Published

2016-01-20

How to Cite

SUN, X., ZHANG, H., ZOU, W. and CHEN, Y., 2016. Experimental study on hplc determination of astragaloside in qishen huoxue granule and its protective effect in septic rats . Bioscience Journal [online], vol. 32, no. 1, pp. 255–261. [Accessed30 November 2022]. DOI 10.14393/BJ-v32n1a2016-29581. Available from: https://seer.ufu.br/index.php/biosciencejournal/article/view/29581.

Issue

Section

Health Sciences