Production and partial purification of antifungal chitinase from Bacillus cereus VITSD3

C. Subathra Devi; V. Mohana Srinivasan; B. Archana; Steffi Susan Roy; S. Jemimah Naine

doi:10.14393/BJ-v31n3a2015-26263

Authors

C. Subathra Devi Vellore Institute of Technology
V. Mohana Srinivasan Vellore Institute of Technology
B. Archana Vellore Institute of Technology
Steffi Susan Roy Vellore Institute of Technology
S. Jemimah Naine Vellore Institute of Technology

DOI:

https://doi.org/10.14393/BJ-v31n3a2015-26263

Keywords:

chitinaseBacillus cereus VITSD3, antifungal activity, chitin, Aspergillus, partial purification.

Abstract

The current work was designed to isolate and characterize chitin degrading bacteria. Among the 55 bacterial colonies isolated from 7 different soil samples, 4 isolates were capable of degrading chitinase, among which one strain VITSD3 was found to be potent. Based on the morphological, biochemical and molecular characterization of VITSD3 the isolate was confirmed as Bacillus cereus (Genbank accession number: KC961638), designated as Bacillus cereus VITSD3. The crude enzyme had a total activity of 220 U, precipitated with 44.8 U and 22.5 U for dialysed sample. The hydrolysed product NAG (N-Acetyl Glucosamine) from chitin was analysed by high-pressure liquid chromatography (HPLC).The molecular weight of the chitinase was determined using SDS PAGE and found to be 55 kDa. The partially purified chitinase produced from Bacillus cereus VITSD3 showed antifungal activity against Aspergillus fumigatus (18 mm), Aspergillus niger (6 mm) and Aspergillus flavus (15 mm). Hence the investigation suggests a potential benefit of partially purified chitinase extracted from Bacillus cereus VITSD3 which will serve as an excellent antifungal potential with therapeutic use.