Potential inhibition of glycation and free radical scavenging activities of purple sweet potato extracts

Abstract

Purple sweet potato (PSP) contains antioxidant compounds and it can be used to prevent oxidative damage to cellular components of human body. The research purpose is to find out the potential of PSP extract on inhibiting glycation process and free radicals scavenging activities. Purple sweet potato was extracted using ethanol 25, 50, and 75% (PSP25, PSP50, PSP75) and then it was analyzed for free radical scavenging activities and antiglycation in forming advanced glycation end products (AGEs) using spectrophotometric method. Then all the collected data were examined with one-way ANOVA (p<0.05). The results showed that PSP extract has antioxidant activities and antiglycation properties. Based on IC50 values, PSP75 extract has a lower IC50 value compared to PSP25 and PSP50 (P <0.05) and has better activity in scavenging DPPH, hydroxyl, and superoxide radicals. This potentiality was shown by the IC₅₀ value of each PSP extract. The value of IC₅₀ of scavenging DPPH radical acitivity for PSP25, PSP50, PSP75 extracts was respectively 281.08, 254.94, and 241.30 mg/mL. The value of IC₅₀ scavenging hydroxyl radicals was respectively 1.03, 088, and 0,79 mg/mL, and the IC₅₀ value of scavenging radicals of superoxide anion was respectively 1.10, 0.97, and 0.82 mg/mL. The absorbance value of PSP75 in the BNT test and Fluorescence intensity are lower than PSP25 and PSP50, so that PSP75 extract is better at inhibiting glycation reaction. It can be concluded that the PSP extract has the potential in the inhibition of the glycation reaction and in the activity of elimination of free radicals (DPPH, hydroxyl, and superoxide radicals).