The probable radioprotective role of Acacia nilotica L. against biochemical and cytogenetic disorders induced in gamma irradiated male rats

Sameh Soliman Tawfik; Wael Aly El khouly; Sherien Abdelwhab Montaser; Manal Ramadan Mohammed

doi:10.14393/BJ-v39n0a2023-65513

Autores

Sameh Soliman Tawfik National Centre for Radiation Research and Technology https://orcid.org/0000-0002-0625-5640
Wael Aly El khouly Nuclear and Radiation Safety Research Center https://orcid.org/0000-0003-3580-5311
Sherien Abdelwhab Montaser Egyptian Atomic Energy Authority https://orcid.org/0000-0001-8033-1018
Manal Ramadan Mohammed National Centre for Radiation Research and Technology https://orcid.org/0000-0002-0664-278X

DOI:

https://doi.org/10.14393/BJ-v39n0a2023-65513

Palavras-chave:

Acacia nilotica, Genetics, Histopathology, Radioprotector, Rats, Testis, γ-rays.

Resumo

Gum Arabic (Acacia nilotica L.) is a respected plant that has many nutrients and curative practices. It hinders, improves, or manages many disorders. The radio-protective activity of Acacia nilotica was investigated against γ-rays-induced testicle damage in rats. Twenty-four rats were correspondingly distributed into 4 groups; control, Acacia nilotica (15mg/kg, daily for 30 days), γ-irradiated (5Gy γ-rays, single dose) and Acacia nilotica plus γ-rays treated groups. The plasma testosterone and total antioxidant status (TAS) were estimated. Lipid peroxidation; malondialdehyde (MDA), reduced glutathione (GSH), malondialdehyde (MDA), superoxide dismutase (SOD), also glutathione peroxidase (GPx), catalase (CAT), tumor necrosis factor-α (TNF-α) with interleukin-1β (IL-1β), were determined in the testicle tissues. A testis weight, sperm count and motility, peripheral-blood and bone-marrow micronuclei (PMN and BMN), and frequency of chromosomal aberrations (CAs) were scored. A significant decline in the levels of plasma testosterone with TAS observed in the γ-irradiated rats. The results also showed significantly increased levels of testicle MDA, inflammatory markers, PMN, BMN and CAs frequencies and decrease in testes weight, sperm count and motility and levels of testicle antioxidants markers in gamma irradiated group. All these biochemical and fertility indices results were significantly enhanced in the Acacia nilotica plus γ-rays treated groups. However, the possible alleviate activity of Acacia nilotica on γ-rays-induced testicle injury in rats has not previously conversed, and this is the topic of this study.

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