Jian-Pi-Yi-Shen drug-containing serum promotes proliferation and activity of mouse osteoblasts

Yuli Cai; Xiaochen Liu; Liang Ma; Wenxue Lv; Weiguo Wang; Xingcai Zhang

doi:10.14393/BJ-v39n0a2023-64818

Autores

Yuli Cai Shandong University of Traditional Chinese Medicine https://orcid.org/0000-0002-3487-6540
Xiaochen Liu Shandong University of Traditional Chinese Medicine
Liang Ma Shandong University of Traditional Chinese Medicine
Wenxue Lv Shandong University of Traditional Chinese Medicine
Weiguo Wang Shandong University of Traditional Chinese Medicine
Xingcai Zhang Shandong University of Traditional Chinese Medicine https://orcid.org/0000-0002-8084-8508

DOI:

https://doi.org/10.14393/BJ-v39n0a2023-64818

Palavras-chave:

Nitric Oxide, Osteoporosis.

Resumo

Jian-Pi-Yi-Shen (JPYS) decoction is a traditional Chinese herbal formula. The present study aimed to investigate the effect of JPYS drug-containing serum on the proliferation and activity of mouse osteoblasts. Sprague‒Dawley rats were fed JPYS decoction, calcium supplement, or normal saline for 6 weeks, and the serum was collected. Mouse osteoblasts were treated with JPYS drug-containing serum, calcium supplement serum, or blank control serum. Cell proliferation was assayed using thiazolyl blue tetrazolium bromide. Levels of alkaline phosphatase, nitric oxide, and nitric oxide synthase in the culture medium were measured. The JPYS drug-containing serum significantly improved the proliferation of osteoblasts compared to the blank control serum and the calcium supplement serum. It also significantly increased the levels of alkaline phosphatase in the culture medium compared to the blank control serum and the calcium supplement serum. Treatment with JPYS drug-containing serum for 48 h and 72 h significantly increased the nitric oxide (NO) concentration in the culture medium compared to the blank control serum. The NOS activity of the osteoblasts was significantly increased by JPYS drug-containing serum compared to blank control serum and calcium supplement serum. All these results were enhanced that the JPYS decoction promotes the proliferation and activity of mouse osteoblasts. These effects may be the underlying mechanisms of JPYS decoction in treating osteoporosis.

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Referências

ARMAS, L.A. and RECKER, R.R. Pathophysiology of osteoporosis: new mechanistic insights. Endocrinology and Metabolism Clinics of North America. 2012, 41(3), 475-486. https://doi.org/10.1016/j.ecl.2012.04.006

BACCARO, L.F., et al. The epidemiology and management of postmenopausal osteoporosis: a viewpoint from Brazil. Clinical Interventions in Aging. 2015, 10, 583-591. https://doi.org/10.2147/cia.s54614

BECERRIL, S., et al. Functional Relationship between Leptin and Nitric Oxide in Metabolism. Nutrients. 2019, 11(9), 2129. https://doi.org/10.3390/nu11092129

COSTA, R.L.B., HAN, H.S. and GRADISHAR, W.J. Targeting the PI3K/AKT/mTOR pathway in triple-negative breast cancer: a review. Breast Cancer Research and Treatment. 2018. 169(3), 397-406. https://doi.org/10.1007/s10549-018-4697-y

DENG, X., et al. The combined effect of oleonuezhenide and wedelolactone on proliferation and osteoblastogenesis of bone marrow mesenchymal stem cells. Phytomedicine. 2019, 65, 153103. https://doi.org/10.1016/j.phymed.2019.153103

GAO, Z., et al. Study of osteoporosis treatment principles used historically by ancient physicians in Chinese Medicine. Chinese Journal of Integrative Medicine. 2013, 19(11), 862-868. https://doi.org/10.1007/s11655-013-1328-z

GUIZONI, D.M., et al. Modulation of endothelium-derived nitric oxide production and activity by taurine and taurine-conjugated bile acids. Nitric Oxide. 2020, 94, 48-53. https://doi.org/10.1016/j.niox.2019.10.008

HE, J.B., CHEN, M.H. and LIN, D.K. New insights into the tonifying kidney-yin herbs and formulas for the treatment of osteoporosis. Archives of Osteoporosis. 2017, 12(1), 14. https://doi.org/10.1007/s11657-016-0301-4

KALYANARAMAN, H., SCHALL, N. and PILZ, R.B. Nitric oxide and cyclic GMP functions in bone. Nitric Oxide. 2018, 76, 62-70. https://doi.org/10.1016/j.niox.2018.03.007

LANE, J.M., RUSSELL, L. and KHAN, S.N. Osteoporosis. Clinical Orthopaedics and Related Research. 2000, 372, 139-150. https://doi.org/10.1097/00003086-200003000-00016

LIU, C.Y., et al. Rehmanniae Radix in osteoporosis: A review of traditional Chinese medicinal uses, phytochemistry, pharmacokinetics and pharmacology. Journal of Ethnopharmacology. 2017, 198, 351-362. https://doi.org/10.1016/j.jep.2017.01.021

LIU, Y., LIU, J.P. and XIA, Y. Chinese herbal medicines for treating osteoporosis. Cochrane Database of Systematic Reviews. 2014, 3, Cd005467. https://doi.org/10.1002/14651858.CD005467.pub2

LORENTZON, M. Treating osteoporosis to prevent fractures: current concepts and future developments. Journal of Internal Medicine. 2019, 285(4), 381-394. https://doi.org/10.1111/joim.12873

MILLER, P.D. Management of severe osteoporosis. Expert Opinion on Pharmacotherapy. 2016, 17(4), 473-488. https://doi.org/10.1517/14656566.2016.1124856

MUHAMMAD, R.N., SALLAM, N. and EL-ABHAR, H.S. Activated ROCK/Akt/eNOS and ET-1/ERK pathways in 5-fluorouracil-induced cardiotoxicity: modulation by simvastatin. Scientific Reports. 2020, 10(1), 14693. https://doi.org/10.1038/s41598-020-71531-8

SASAKI, S., et al. A Role of Intestinal Alkaline Phosphatase 3 (Akp3) in Inorganic Phosphate Homeostasis. Kidney Blood Press Res, 2018, 43(5), 1409-1424. https://doi.org/10.1159/000493379

SHU, B., SHI, Q. and WANG, Y.J. Shen (Kidney)-tonifying principle for primary osteoporosis: to treat both the disease and the Chinese medicine syndrome. Chinese Journal of Integrative Medicine. 2015, 21(9), 656-661. https://doi.org/10.1007/s11655-015-2306-z

SILLER, A.F. and WHYTE, M.P. Alkaline Phosphatase: Discovery and Naming of Our Favorite Enzyme. Journal of Bone and Mineral Research. 2018, 33(2), 362-364. https://doi.org/10.1002/jbmr.3225

SRIVASTAVA, M. AND DEAL, C. Osteoporosis in elderly: prevention and treatment. Clinics in Geriatric Medicine. 2002, 18(3), 529-555. https://doi.org/10.1016/s0749-0690(02)00022-8

XU, H., et al. The use of preventive strategies for bone loss. The American Journal of Chinese Medicine. 2005, 33(2), 299-306. https://doi.org/10.1142/S0192415X05002916

YAN, G.L., et al. Progress of serum pharmacochemistry of traditional Chinese medicine and further development of its theory and method. Zhongguo Zhong Yao Za Zhi. 2015, 40(17), 3406-3412.

ZHAO, Y., VANHOUTTE, P.M. and LEUNG, S.W. Vascular nitric oxide: Beyond eNOS. Journal of Pharmacological Sciences. 2015, 129(2), 83-94. https://doi.org/10.1016/j.jphs.2015.09.002