Hiclato de doxiciclina pode causar danos ao epitélio germinativo testicular em ratos Wistar
DOI:
https://doi.org/10.14393/BJ-v39n0a2023-64399Palavras-chave:
Antibiotics, Histology, Male Infertility, Morphometry, Reproduction.Resumo
A doxiciclina antimicrobiana de amplo espectro atua como inibidor da síntese proteica e é amplamente utilizada no tratamento clínico de diversas infecções por microrganismos sensíveis ao fármaco, bem como na alimentação animal. Sua lipossolubilidade garante sua alta biodisponibilidade tecidual e seu uso está associado a diversas alterações bioquímicas no organismo e efeitos potencialmente adversos na reprodução. Este estudo tem como objetivo avaliar os efeitos da ação da doxiciclina na espermatogênese para fornecer uma análise completa dos compartimentos tubular e intersticial e identificar possíveis alterações no parênquima testicular. Ratos Wistar machos adultos foram divididos em três grupos: um controle (água) e dois tratados com doxiciclina nas doses de 10mg/kg e 30mg/kg, por 30 dias. Após a eutanásia e processamento das amostras, foram avaliados os seguintes parâmetros: a) diâmetro tubular e altura do epitélio seminífero; b) proporções volumétricas (%) e volumes (mL) dos componentes do parênquima testicular; c) contagem de populações de células germinativas testiculares; d) avaliação da viabilidade celular. Os resultados da avaliação comparativa entre os grupos experimentais demonstraram aumento significativo no diâmetro e área do lúmen tubular e redução na contagem de espermatogônias no grupo experimental que recebeu hidrato de doxiciclina na dose de 30mg/kg. No mesmo grupo experimental, foi encontrado um aumento no rendimento global da espermatogênese como consequência do aumento do índice mitótico.
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Copyright (c) 2023 João Vitor Lopes-Ferreira, Jullyana Costa Machado, Fernanda Carolina Riberio Dias, Luciana Schulthais Altoé, Reggiani Gonçalves Vilela, Sérgio Luis Pinto da Matta, Angelica de Oliveira Gomes, Marcos de Lucca Moreira Gomes
Este trabalho está licenciado sob uma licença Creative Commons Attribution 4.0 International License.
