Teste rápido para detectar resistência a herbicidas inibidores de ALS em arroz-daninho e capim-arroz em um bioensaio à base de ágar
DOI:
https://doi.org/10.14393/BJ-v39n0a2023-63353Palavras-chave:
BAP, Echinochloa spp., Growth medium, Oryza sativa, Tiller regeneration.Resumo
A resistência aos inibidores da acetolactato sintase (ALS) aumentou recentemente no Sul do Brasil, onde as plantas daninhas capim-arroz e arroz-daninho desenvolveram resistência a imazapyr+imazapic. O objetivo foi avaliar um meio de crescimento adequado para regeneração de tecidos, bem como discriminar doses de imazapyr+imazapic incluídos no meio de cultura para distinguir biótipos resistentes e suscetíveis de arroz-daninho e capim-arroz em bioensaios à base de ágar. Experimentos em estufa foram conduzidos para detectar biótipos resistentes (R) e suscetíveis (S) de arroz-daninho e capim-arroz aos inibidores de ALS, e bioensaios foram conduzidos para avaliar um meio de crescimento adequado para regeneração de tecido de capim-arroz e determinar a concentração de herbicida necessária para distinguir biótipos R e S. O meio de cultura que proporcionou um crescimento adequado de capim-arroz foi MS 50% com a adição de benzilamino-purina. A regeneração in vitro com o meio de crescimento contendo imazapyr+imazapic permitiu discriminar R e S biótipos de arroz-daninho e capim-arroz. A concentração necessária para o controle satisfatório de explantes suscetíveis de capim-arroz e arroz daninho cultivados in vitro foi de 0,9 μM e 1,3 μM de imazapyr+imazapic, respectivamente. O bioensaio usando regeneração de explantes in vitro gera uma oportunidade de predizer a eficácia de imazapyr+imazapic.
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Copyright (c) 2023 Dirceu Agostinetto, Daniela Tessaro, Maicon Fernando Schmitz, Matheus Bastos Martins, Leandro Vargas, André da Rosa Ulguim
Este trabalho está licenciado sob uma licença Creative Commons Attribution 4.0 International License.
