Chemical constituents and in vivo preliminary evaluation of the toxicological activity of Ouratea spectabilis (OCHNACEAE) and Clitoria guianensis (Fabaceae) leaves

Frank Bruno Vieira de Sousa; Állefe Barbosa Cruz; Daniela Francisca Soares; Camila Luiza Cunha; Juliana Cristina Holzbach

doi:10.14393/BJ-v39n0a2023-62323

Autores

Frank Bruno Vieira de Sousa Universidade Federal do Tocantins https://orcid.org/0000-0003-3215-2351
Állefe Barbosa Cruz Universidade Federal do Tocantins
Daniela Francisca Soares Universidade Federal do Tocantins https://orcid.org/0000-0002-8641-5674
Camila Luiza Cunha Universidade Federal de São Paulo https://orcid.org/0000-0002-7184-0870
Juliana Cristina Holzbach Universidade Federal do Tocantins https://orcid.org/0000-0003-2489-9359

DOI:

https://doi.org/10.14393/BJ-v39n0a2023-62323

Palavras-chave:

Artemia salina, Cerrado, Kaempferitrin.

Resumo

As espécies Clitoria guianensis e Ouratea spectabilis, encontradas no Cerrado brasileiro, são utilizadas na medicina popular, apesar de não apresentarem estudos químicos e biológicos reportados na literatura. O presente trabalho busca investigar a toxicidade frente ao microcrustáceo Artemia salina, dos extratos destas espécies, assim como a composição química do extrato hexânico das folhas de O. spectabilis e da fração EtOAc das folhas de C. guianensis. O estudo químico da fração EtOAc das folhas de C. guianensis resultou no isolamento de um flavonoide conhecido como Kaempferitrin. A análise do extrato hexânico das folhas de O. spectabilis, por CG-EM, sugere a presença de 13 substâncias conhecidas. Os extratos Hex, EtOAc e EtOH bruto de C. guianensis mostraram alta e moderada toxicidade frente a Artemia salina, respectivamente, com valores de dose letal (DL₅₀) de 43.7, 25.4 e 233.4 mg.L^-1. O extrato acetônico das folhas de O. spectabilis apresentou moderada toxicidade frente a Artemia salina com DL₅₀ de 114.8 mg.L^-1.

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