Toxicity of Bacillus sp. (Bacillales: Bacillaceae) on the fungus gnats, Bradysia aff. ocellaris larvae (Diptera: Sciaridae)
DOI:
https://doi.org/10.14393/BJ-v39n0a2023-59878Palavras-chave:
Biological Control, Bti, Strawberry, Sustainable management.Resumo
Bacillus thuringiensis (Bt) Berliner tem potencial para uso no manejo de insetos. Seu uso pode ser uma alternativa para o manejo da Bradysia aff. ocellaris (Comstock), considerada uma das principais pragas do morangueiro em sistema sem solo. Portanto, o objetivo deste trabalho foi avaliar a toxicidade de diferentes bactérias sobre B. aff. ocellaris em bioensaios de laboratório e estufa. Os seguintes isolados foram usados nos experimentos: Bacillus circulans (Bc), B. thuringiensis var. oswaldo cruzi (Bto) ou B. thuringiensis var. israelensis (Bti) e B. thuringiensis var. kurstaki (Btk). No laboratório, larvas de B. aff. ocellaris mostraram alta suscetibilidade ao isolado Bti (92% de mortalidade) 14 dias após a exposição ao tratamento (DAET). Em contraste, os isolados Bc, Bto e Btk apresentaram mortalidade inferior a 32%, não diferindo do tratamento controle (água - mortalidade de 22%). De acordo com as curvas concentração-resposta, os valores de concentração letal LC50 e LC90 foram 4 x 106 UFC.mL-1 e 4 x 1015 UFC.mL-1. Ao aplicar Bti (4 × 1012 CFU.mL-1) na base de plantas de morangueiro crescendo em vasos plásticos contendo substrato de planta comercial, houve redução de 26% na emergência de B. aff. ocellaris foi observado. De acordo com esses resultados, o isolado Bti é considerado promissor para a formulação de bioinseticidas à base de Bt para o manejo de B. aff. ocellaris na cultura do morangueiro.
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Copyright (c) 2023 Adriane da Fonseca Duarte, Juliano Lessa Pinto Duarte, Liliane Nachtigall Martins, Lucas Raphael da Silva, Nycole de Souza Cunha, Fábio Pereira Leivas Leite, Uemerson Silva da Cunha, Daniel Bernardi
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