INVESTIGAÇÃO DO POSSÍVEL EFEITO PROTETOR DA FOLHA DE Smilax fluminensis STEUD. EM CAMUNDONGOS SUBMETIDOS AO ESTRESSE OXIDATIVO PELO PARACETAMOL
DOI:
https://doi.org/10.14393/BJ-v37n0a2021-53862Palavras-chave:
Acetaminophen, Free Radicals, Hepatotoxicity, Smilax fluminensis.Resumo
Paracetamol (PCM), um fármaco amplamente utilizado pela população como antipirético e analgésico. Se administrado em altas doses pode causar dano hepático, levando a hepatoxicidade. O gênero Smilax, encontradas em regiões temperadas e tropicais, é tradicionalmente utilizado pela população através do extrato das folhas e raízes para diversas afecções, como no tratamento da sífilis, diabetes, asma e como ação diurética. Através disto, utilizaram-se os extratos das folhas da Smilax fluminensis para avaliar o efeito protetor frente ao estresse oxidativo induzido através de uma alta dose de PCM em camundongos que receberam o medicamento e após receberam o tratamento com extrato bruto e frações. Foram realizadas análise do plasma através da aspartato aminotransferase (AST), alanina aminotransferase (ALT), glicose, triglicérides e colesterol, além de técnicas bioquímicas como, catalase (CAT), glutationa-S-transferase (GST), glutationa reduzida (GSH), ácido ascórbico (ASA), substâncias reativas ao ácido tiobarbitúrico (TBARS) e proteínas carboniladas (CARBONIL) de fígado, cérebro e rins. A fração 1 do extrato foi a mais promissora, diminuindo os níveis plasmáticos da AST e ALT, os níveis de CAT e GST do fígado, juntamente com a GSH e no tecido renal e cerebral houve uma diminuição das proteínas carboniladas (PCM+F1 versus PCM). Além disso, a fração 1 mostrou-se hipoglicêmica e hipocolesterolêmica. Conclui-se que a fração 1 das folhas da Smilax fluminensis possui boa atividade antioxidante frente aos danos causados pela alta dose de paracetamol.
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Copyright (c) 2021 Ana Paula Simões da Cunha, Larissa Scremin Ferreira, Ana Júlia Pasuch Gluzezak, Edith Eunice Arthur Petrica, Adilson Paulo Sinhorin, Valéria Dornelles Gindri Sinhorin
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