Process optimization for enhanced production of cellulases form locally isolated fungal strain by submerged fermentation
DOI:
https://doi.org/10.14393/BJ-v37n0a2021-53815Palavras-chave:
Aspergillus niger, Cellulase, CMCase, FPase, Molecular CharacterizationResumo
Cellulase has myriad applications in various sectors like pharmaceuticals, textile, detergents, animal feed and bioethanol production, etc. The current study focuses on the isolation, screening and optimization of fungal strain through one factor at a time technique for enhanced cellulase production. In current study sixteen different fungal cultures were isolated and the culture which quantitatively exhibits higher titers of cellulase activity was identified both morphologically and molecularly by 18S rDNA and designated as Aspergillus niger ABT11. Different parameters like fermentation medium, volume, temperature, pH and nutritional components were optimized. The highest CMCase and FPase activities was achieved in 100ml of M5 medium in the presence of 1% lactose and sodium nitrate at 30 oC, pH5 after 72 hours. The result revealed A. niger can be a potential candidate for scale up studies.
Downloads
Referências
ABD-ELRSOUL, R.M.M.A. and BAKHIET, S.E.A. Optimization of factors influencing cellulase production by some indigenous isolated fungal species. Jordan Journal of Biological Sciences. 2018, 11(2), 31-36.
AKULA, S. and GOLLA, N. Optimization of cellulase production by Aspergillus niger isolated from forest soil. The open Biotechnology Journal. 2018, 12(1), 256-269. http://dx.doi.org/10.2174/1874070701812010256
BHAT, M. Cellulases and related enzymes in biotechnology. Biotechnology Advances. 2000, 18(5), 355-383. https://doi.org/10.1016/S0734-9750(00)00041-0
BRADFORD, M.M.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Analytical Biochemistry. 1976, 72(1-2), 248-254. https://doi.org/10.1006/abio.1976.9999
CARLILE, M. J., et al. The Fungi. 2nd ed. London: Academic Press, 2001.
CHELLAPANDI, P. and JANI, H.M. Production of endoglucanase by the native strains of Streptomyces isolates in submerged fermentation. Brazilian Journal of Microbiology. 2008, 39(1), 122-127. https://dx.doi.org/10.1590%2FS1517-838220080001000026
DA SILVA, V.C.T., et al. Effect of pH, Temperature, and Chemicals on the Endoglucanases and β-Glucosidases from the Thermophilic Fungus Myceliophthora heterothallica F. 2.1. 4. Obtained by Solid-State and Submerged Cultivation. Biochemistry Research International. 2016, 2016, 1-9. https://doi.org/10.1155/2016/9781216
DAMISA, D., AMEH, J. and EGBE, N. Cellulase production by native Aspergillus niger obtained from soil environments. Fermentation Technology and Bioengineering. 2011, 1, 62-70.
EL-HADI, A.A., et al. Optimization of cultural and nutritional conditions for carboxy methyl cellulase production by Aspergillus hortai, Journal of Radiation Research and Applied Sciences. 2014, 7(1), 23-28. https://doi.org/10.1016/j.jrras.2013.11.003
GAO, J., et al. Production and characterization of cellulolytic enzymes from the thermoacidophilic fungal Aspergillus terreus M11 under solid-state cultivation of corn stover. BioResource Technology. 2008, 99(16), 7623-7629. https://doi.org/10.1016/j.biortech.2008.02.005
GEOFFRY, K. and ACHUR, R.N. Screening and production of lipase from fungal organisms. Biocatalyst and Agriculture Biotechnology. 2018, 14, 241-253 https://doi.org/10.1016/j.bcab.2018.03.009
GORI, M.I. and MALANA, M.A. Production of carboxymethyl cellulase from local isolate of Aspergillus species. Pakistan Journal of Life and Social Sciences. 2010, 8(1), 1-6.
HAQ, I., et al. Sugar cane bagasse pretreatment: an attempt to enhance the production potential of cellulases by Humicola insolens TAS-13. Biokemistri. 2006, 18(2), 83-88. https://doi.org/10.4314/biokem.v18i2.56396
HAQ, I., et al. Cotton saccharifying activity of cellulases produced by co-culture of Aspergillus niger and Trichoderma viride. Research Journal of Agriculture and Biology Sciences. 2005, 1(3), 241-245.
HUSSAIN, A., et al. Cellulolytic enzymatic activity of soft rot filamentous fungi Paecilomyces variotii. Advances in BioResearch. 2012, 3(3), 10-17.
IRFAN, M., et al. UV mutagenesis of Aspergillus niger for enzyme production in submerged fermentation. Pakistan Journal of Biochemistry and Molecular Biology. 2011, 44(4), 137-140.
KARNCHANATAT, A., et al. A novel thermostable endoglucanase from the wood-decaying fungus Daldinia eschscholzii. Enzyme and Microbial Technology. 2008, 42(5), 404-413. https://doi.org/10.1016/j.enzmictec.2007.11.009
KARTHIKEYAN. N. and PALANI, P.S.M. Screening, identifying of Penicillium K-P strain and its cellulase producing conditions. Journal of Ecobiotechnology. 2010, 2(10), 4-7.
MMANGO-KASEKE, Z., et al. Optimization of cellulase and xylanase production bymicrococcus species under submerged fermentation. Sustainability. 2016, 8(11), 1168(1-15) https://doi.org/10.3390/su8111168
KIM, D., et al. Identification and molecular modeling of a family 5 endocellulase from Thermus caldophilus GK24, a cellulolytic strain of Thermus thermophilus. International Journal of Molecular Sciences. 2006, 7(12), 571-589. https://doi.org/10.3390/i7120571
KUMAR S., et al. MEGA X: Molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution. 2018, 35(6), 1547-1549. https://doi.org/10.1093/molbev/msy096
LYND, L.R., et al. Microbial cellulose utilization: fundamentals and biotechnology. Microbiology and Molecular Biology Reviews. 2002, 66(3), 506-577. https://doi.org/10.1128/MMBR.66.3.506-577.2002
MALIK, S.K., et al. Optimization of process parameters for the biosynthesis of cellulases by Trichoderma viride. Pakistan Journal Botany. 2010, 42(6), 4243-4251.
MILLER, G.L. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry. 1959, 31(3), 426-428. https://doi.org/10.1021/ac60147a030
NISAR, K., et al. Hyper production of carboxy methyl cellulase by Thermomyces dupontii utilizing physical and chemical mutagenesis. Revista Mexicana de Ingeniería Química. 2020, 19(2), 617-625. https://doi.org/10.24275/rmiq/Bio823
OLIVEIRA, P., et al. Cocoa shell for the production of endoglucanase by Penicillium roqueforti ATCC 10110 in solid state fermentation and biochemical properties. Revista Mexicana de Ingeniería Química. 2019, 18(3), 777-787. https://doi.org/10.24275/uam/izt/dcbi/revmexingquim/2019v18n3/Oliveira
PADMAVATHI, T., et al. Optimization of the medium for the production of cellulases by Aspergillus terreus and Mucor plumbeus. European Journal of Experimental Biology. 2012, 2(4), 1161-1170.
POTHIRAJ, C. and EYINI, M. Enzyme activities and substrate degradation by fungal isolates on cassava waste during solid state fermentation. Mycobiology. 2007, 35(4), 196-204.
REDDY, P.L.N., et al. Screening, identification and isolation of cellulolytic fungi from soils of Chittoor district, India. International Journal of Current Microbiology and Applied Sciences. 2014, 3(7), 761-77.
SINGH, A., et al. Production of cellulases by Aspergillus heteromorphus from wheat straw under submerged fermentation. International Journal of Civil and Environmental Engineering. 2009, 1(1), 23.26.
TAO, Y.M., et al. Purification and properties of endoglucanase from a sugar cane bagasse hydrolyzing strain, Aspergillus glaucus XC9. Journal of Agricultural and Food Chemistry. 2010, 58(10), 6126-6130. https://doi.org/10.1021/jf1003896
YOON, L.W., et al. Fungal solid-state fermentation and various methods of enhancement in cellulase production. Biomass and Bioenergy. 2014, 2014(67), 319–338. http://dx.doi.org/10.1016/j.biombioe.2014.05.013
Downloads
Publicado
Como Citar
Edição
Seção
Licença
Copyright (c) 2021 Roheena Abdullah, Ammara Akhtar, Kinza Nisar, Afshan Kaleem, Mehwish Iqtedar, Tehreema Iftikhar, Faiza Saleem, Farheen Aslam
Este trabalho está licenciado sob uma licença Creative Commons Attribution 4.0 International License.
