Biological Trace Information Extracted from Bioaerosols Using NGS Analysis

Panyapon Pumkaeo; Wenhao Lu; Youki  Endou; Tomofumi Mizuno; Junko Takahashi; Hitoshi Iwahashi

doi:10.14393/BJ-v37n0a2021-53678

Autores

Panyapon Pumkaeo Gifu University
Wenhao Lu Gifu University
Youki Endou Gifu University
Tomofumi Mizuno Gifu University
Junko Takahashi National Institute of Advanced Industrial Science and Technology
Hitoshi Iwahashi Gifu University

DOI:

https://doi.org/10.14393/BJ-v37n0a2021-53678

Palavras-chave:

Biological trace information, Bioaerosol, Next Generation Sequencing

Resumo

Os bioaerosóis são partículas atmosféricas com traços biológicos, como vírus, bactérias, fungos e material vegetal, como pólen e detritos vegetais. Neste estudo, analisamos as informações biológicas em bioaerossóis usando o sequenciamento de última geração do DNA traço. As amostras foram coletadas usando um amostrador Andersen e separadas em dois grupos de acordo com o tamanho do material particulado (PM): pequeno (PM2,5) e grande (PM10). A amplificação e o sequenciamento do gene 16S rDNA bacteriano, região espaçador 1 transcrita interna procariótica (ITS1) e sequência de DNA de um gene de cloroplasto vegetal (rbcL) foram realizados usando vários conjuntos de iniciadores específicos visando sequências animais e vegetais.

Muita informação bacteriana foi detectada a partir dos bioaerosóis. As bactérias mais abundantes em várias amostras foram das Actinobacteria (classe), Alphaproteobacteria, Bacilli e Clostridia. Para a detecção de animais usando espaçador interno transcrito 1, apenas fungos não cultivados foram detectados em mais da metade dos acertos, com alto número de Cladosporium sp. nas amostras. Para a identificação da planta, as informações ITS1 correspondiam apenas às espécies de fungos. No entanto, o direcionamento da região rbcL revelou diversas informações sobre plantas, como Medicago papillosa. Em conclusão, traços de bactérias, fungos e plantas podem ser detectados nos bioaerossóis, mas não nos animais que usam nossos primers.

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