CARACTERIZAÇÃO E PLASTICIDADE DE CÉLULAS –TRONCO MESENQUIMAIS DE GELÉIA DE WHARTON DE CORDÃO UMBILICAL DE CAPRINO
DOI:
https://doi.org/10.14393/BJ-v37n0a2021-50386Palavras-chave:
Cell Culture, Flow Cytometry, Lineage Differentiation, Mesenchymal Stem Cells, Umbilical Cord Matrix.Resumo
RESUMO: Células-tronco mesenquimais (MSCs) de vários sítios anatômicos foram descritas, caracterizadas e utilizadas em modelos terapêuticos. As células-tronco do cordão umbilical (UC-MSCs) e anexos fetais, incluindo as da geleia de Wharton (WJ-MSCs) são de fácil aquisição, disponibilidade, não requer procedimento invasivo, não apresentam risco para os doadores e não apresenta limitação ética. O objetivo deste estudo foi analisar a plasticidade das células-tronco mesenquimais da geleia de Wharton (WJ-MSCs) do cordão umbilical de caprinos, avaliando seu comportamento in vitro e caracterizando estas células imunofenotipicamente. Assim, foram realizados ensaios de unidades formadoras de colônias, viabilidade e curva de crescimento celular, análise por citometria de fluxo e o potencial de plasticidade. As células da matriz do cordão umbilical caprino exibiram morfologia fibroblastóide com formação de colônias e capacidade de autorenovação, sempre mantendo seu estado indiferenciado até a oitava passagem (P8). A cinética celular apresentou as fases LAG, LOG, PLATEAU e DECAIMENTO. As células WJ-MSCs de caprino, durante a sétima passagem (P7), responderam in vitro a diferenciação celular por meios indutores para as linhagens, osteogênica, adipogênica e condrogênica, resultando em osteoblastos, adipócitos e condrócitos respectivamente, apresentando características tridimensionais semelhantes aos tecidos. A citometria de fluxo demostrou a expressão de CD90 e CD105; ausência da expressão CD14. Conclui-se que a população de células isoladas a partir da geleia de Wharton de caprino constitui uma amostra representativa de células-tronco, com grandes possibilidades no campo da medicina regenerativa e reprodutiva.
PALAVRAS-CHAVE: Matriz de cordão umbilical. Cultura de célula. Citometria de fluxo. Células –tronco mesenquimais. Diferenciação de linhagem
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Copyright (c) 2021 Gustavo Cardoso da Silva Neves, Napoleão Martins Argôlo Neto, Maíra Soares Ferraz, Clautina Ribeiro de Moraes da Costa, Andressa Rêgo da Rocha, Huanna Waleska Soares Rodrigues, Felipe de Carvalho Nunes, Camila Ernanda Sousa de Carvalho, Miguel Ferreira Cavalcante Filho, Danilo José Ayres de Menezes, Maria Acelina Martins de Carvalho
Este trabalho está licenciado sob uma licença Creative Commons Attribution 4.0 International License.
