Antagonistic activity of bacteria from wild honey against Colletotrichum musae, and testing of wild honey as biopesticide spray to control banana anthracnose

Mark Paul Selda  Rivarez; Elizabeth P. Parac; Niño R. Laurel; Benjamin V. Cunanan; Angelie B. Magarro; Shajara Fatima M. Dimasingkil

doi:10.14393/BJ-v37n0a2021-49921

Autores

Mark Paul Selda Rivarez University of the Philippines Los Baños
Elizabeth P. Parac Caraga State University https://orcid.org/0000-0002-1987-9612
Niño R. Laurel University of the Philippines Los Baños
Benjamin V. Cunanan University of the Philippines Los Baños, Los Baños
Angelie B. Magarro University of the Philippines Los Baños
Shajara Fatima M. Dimasingkil Mindanao State University-Maguindanao

DOI:

https://doi.org/10.14393/BJ-v37n0a2021-49921

Palavras-chave:

Bacillus, Banana Anthracnose, Biological Control, Colletotrichum musae, Lactobacillus, Wild Honey.

Resumo

RESUMO: A antracnose é uma doença foliar e frutícola causada por Colletotrichumspp. afetando uma ampla gama de culturas. A infecção ocorre precocemente, seguida de quiescência em frutas e os pesticidas de base química são usados como controle fúngico confiável por muitos anos. Há uma necessidade crescente de um controle seguro e como implicado na Lei de Agricultura Orgânica de 2010 (RA 10068) nas Filipinas. Esse cenário impulsionou o uso de controle alternativo de pragas, como o uso de produtos biológicos e naturais. Neste estudo, sete bactérias foram isoladas de mel silvestre, onde quatro (BC2, BC3, BC6 e BC7) mostraram ser um antagonista efetivo contra Colletotrichum musae em condições in vitro. Essas bactérias foram identificadas como pertencentes ao gênero Lactobacillusspp. (BC2, BC3, BC7) e Bacillusspp. (BC6) com base em testes de utilização de açúcar, crescimento morfológico e cultural em PDPA. Para o teste in vivo, diferentes diluições de mel silvestre foram utilizadas e constatou-se que concentrações mais baixas foram efetivas como pulverização de biopesticidas para prevenir a infecção por antracnose. Este estudo é o primeiro a conduzir isolamento de bactérias antagonistas em mel silvestre e a aplicar o produto natural como biopesticida em frutos pós-colheita.

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