Bioscience Journal

Climate change is expected to reduce the potential distribution of Ceiba glaziovii in Caatinga, the largest area of dry tropical forest in South America

2024-08-16T11:31:30-03:00

Ecological niche modeling is a widely used tool to predict species distribution considering current, past, or future climate change scenarios across different geographic areas. Modeling scenarios allow researchers to assess the impacts of climate change on species distribution and identify priority areas for conservation. This study aimed to model the current and future potential distribution of Ceiba glaziovii under different climate change scenarios in Brazil. The MaxEnt algorithm was used to correlate species occurrence points with bioclimatic variables in current and future climate scenarios. Four General Circulation Models (GCMs) from CMIP6 were employed: BCC-CSM2-MR, CNRM-CM6-1, IPSL-CM6A-LR, and MIROC6, considering optimistic and pessimistic projections. The contribution of variables and model accuracy were assessed using the Jackknife statistical test and the Area Under the Curve (AUC) parameter. AUC values for current and future scenarios demonstrated high accuracy. The bioclimatic variables of precipitation and temperature were the main contributors to determining areas with higher habitat suitability. In the future climate scenario, there was a reduction in areas with good climatic suitability for all four GCMs, considering optimistic and pessimistic projections. Among the areas with high habitat suitability, the IPSL-CM6A-1 model in the optimistic projection showed the smallest reduction, while in the pessimistic scenario, all areas with high suitability disappeared. The species' climatic niche is expected to decrease under all tested climate change scenarios. The central areas of the Caatinga and its transition zones exhibit the highest climatic suitability in current and future scenarios and should be prioritized for the species' conservation.

Pre-and post-emergence herbicide selectivity in peanuts at an early stage

2024-08-07T10:47:15-03:00

Studies on herbicide selectivity to peanuts detected differences according to the evaluated active ingredient and genotypes. This study analyzed the herbicide selectivity of two genotypes at an early stage. Pre-emergence (trifluralin, pendimethalin, diclosulam, s-metolachlor, imazethapyr + flumioxazin, clomazone, sulfentrazone, and imazapic) and post-emergence (imazapic, bentazon, bentazon + imazamox, clethodim, quizalofop-p-ethyl, cloransulam-methyl, s-metolachlor, lactofen, 2,4-D, and carfentrazone) applications were assessed in 1253 OL and 2133 OL breeding lines. The effects of pre- (PRE) and post-emergence (POST) herbicides were the same for both genotypes, with PRE not affecting seedling emergence and shoot and root dry mass. Diclosulam was among the most selective PRE herbicides, while the clomazone treatment caused only mild damage. All tested POST herbicides caused damage for up to 14 days after application (DAA). At 28 DAA, most herbicides exhibited the same damage as the untreated control. Lactofen caused mild damage (11.8%) without reducing plant height and shoot and root dry mass. Diclosulam, clomazone, and lactofen are unregistered for peanut crops in Brazil, and further studies should test their selectivity for peanut cultivars. Screening studies on selectivity to imazapic, 2,4-D, and carfentrazone are also relevant to identifying sources of tolerance in peanut germplasm.

Evaluation of postharvest age and dormancy-breaking methods on Echinochloa crus-galli seed germination

2024-05-17T08:18:10-03:00

Barnyardgrass (Echinochloa crus-galli) is one of the most troublesome weeds in irrigated rice cultivation and has increasingly impacted rainfed crops due to the emergence of herbicide-resistant populations. Understanding its germination dynamics is crucial for developing and implementing effective management strategies. Additionally, since barnyardgrass research relies on growing plants from seeds, its dormancy characteristics are of particular interest. The present study aimed to evaluate the influence of postharvest age on barnyardgrass seed germination and the effectiveness of different dormancy-breaking methods in susceptible and herbicide-resistant populations. Germination rate (G), germination speed index (GSI), and seed viability, assessed using the topographic tetrazolium test, were measured in seed lots with four different postharvest ages: two years, one year, two months, and one day postharvest. The seeds were subjected to 15 dormancy-breaking methods, including temperature variation and the use of solutions containing H₂SO₄, KNO₃, and GA₃. Seeds that were one or two years old showed germination rates exceeding 90%, regardless of the method used. In contrast, seeds aged two months or one day postharvest only germinated when exposed to 40°C for seven days, with G values of 25.2% and 5.9%, respectively. Both herbicide-susceptible and resistant barnyardgrass populations exhibited similar dormancy levels and responses to dormancy-breaking methods. The results indicate that newly harvested seeds have high dormancy levels, and specific methods are only partially effective in overcoming barnyardgrass seed dormancy.

Multifunctional fluorescent Pseudomonas: effects on maize development and tools for their selection

2024-10-01T15:43:59-03:00

Maize is one of the most cultivated cereals worldwide. Despite the low nutrient availability in the soil, high amounts of fertilizers are applied causing economic and environmental impacts. Then, Plant Growth Promoting Rhizobacteria (PGPR) as Fluorescent Pseudomonas can be utilized as an alternative. The present work aims to analyze the effect of Pseudomonas isolates on maize development and production and verify the relationship between growth mechanisms and IAA production. Sixteen Pseudomonas isolates were tested in vitro to produce IAA, ACC deaminase, siderophores, and solubilize Fe and Al phosphates. Three isolates (CBSAL02, CBSAL05, and CBSAL06) were selected for the field experiment, in which an A. brasilense inoculant was the control, generating five treatments with four replications. More than 50% of the isolates demonstrated the tested mechanisms. Only CBSAL05 did not produce siderophore or could fix N. Inoculations with A. brasilense and Pseudomonas isolates increased leaf N content among the selected isolates. The CBSAL06 isolate increased productivity, thus demonstrating the potential use as an inoculant.

Sex-related variations in the morphological structure of puma (Puma concolor Linnaeus, 1771) ear skin

2024-10-01T16:15:45-03:00

The ear tissue of vulnerable felines, such as the puma, holds potential biological material for creating biobanks. However, skin composition can differ significantly between individuals of the of the same species and even between sexes, based on different environments. Therefore, identifying morphological similarities across these populations is crucial for developing accurate protocols. This study aimed to characterize and evaluate the structure and composition of ear skin in both a male and a female puma using histological techniques. Histomorphometric analysis revealed a total thickness of 304.65 µm and 238.95 µm for the male and the female, respectively. The epidermis was notably thinner in the female compared to the male. Specifically, the thickness of the basal, spinous, and corneum layers in the female was 2.62 µm, 10.07 µm, and 3.15 µm, respectively, compared to 3.49 µm, 13.94 µm, and 3.66 µm in the male, respectively. Melanocytes, keratinocytes, and fibroblasts totaled 26, 24, and 50 cells in the male and 21, 25, and 54 cells in the female. Moreover, chondrocytes (male: n=33 and female: n=41) and perichondrium (male=13.99 µm and female=9.05 µm) were observed in the cartilage. These results demonstrate the histomorphometric differences and similarities between a male and a female puma, consistent with observation in other felines. This information is relevant for a targeted approach for establishing biobanks for this species.

Genotypic reaction of chickpea to Fusarium oxysporum and seed sanitary quality

2024-03-22T10:40:45-03:00

This research investigated the reaction of chickpea genotypes to Fusarium oxysporum CML 2878, seed health quality, and seed treatment with Carbendazim + Thiram fungicide. The roots of 15-day-old chickpea seedlings were injured, immersed in fungal suspension (4.5 x 10⁷ conidia/mL), and transplanted. Thirty days later, F. oxysporum incidence in the genotypes, pathogenicity, and fresh mass and length of the root and shoot were evaluated. The conducted seed health test followed the incubation methodology on a paper substrate. The seed health and sand emergence tests used a factorial arrangement with two seed treatments (treated with fungicide and untreated) and nine genotypes. Total emergence and the number of normal and abnormal plants were evaluated. All genotypes were susceptible to F. oxysporum CML 2878, thus yellowing and browning the leaves and reducing root development. The primary fungi associated with the seeds of the studied genotypes were Aspergillus sp., Fusarium sp., Rhizopus sp., and Penicillium sp. The fungicide was highly efficient in fungal control but reduced emergence, weight, seedling height, and the number of normal seedlings in the genotypes. This study demonstrated that the evaluated genotypes were susceptible to F. oxysporum CML 2878 and Fusarium sp., spread by seeds. Although fungicidal control was efficient for Fusarium, it may interfere with chickpea germination and vigor.

Rhizobia inoculation increases pea grain yield: an overview and challenges

2023-09-27T21:20:11-03:00

The aim of this study was to evaluate the contribution of inoculation with microorganisms, mainly rhizobia, on pea (Pisum sativum) production using a systematic literature review approach and a comparative analysis of grain yield to provide information to fill gaps in nontraditional regions of cultivation for this legume. A systematic search strategy was implemented, targeting papers published in scientific journals accessible through the Web of Science® (WoS) database spanning from January 1990 to April 2022. The search terms "Pisum sativum," "inoculation," and "strain" were used. The differences between the pea grain yields from plants inoculated with nitrogen-fixing microorganisms and those from noninoculated plants (control) were analyzed using thirteen field-scale studies. Overall, pea inoculation/coinoculation promoted a greater grain yield (3118 ±210 kg ha^-1) than noninoculation (2338± 127 kg ha^-1), showing the importance of biological nitrogen fixation for legume crop production. In the pursuit of reducing pea production costs, emphasis has been placed on inoculation, highlighting the importance of comprehending the symbiotic relationship between peas and Rhizobium. Furthermore, this research aimed to explore the interaction between Rhizobium and selected microorganisms known to enhance plant growth to identify optimal combinations to boost pea productivity.

Multifunctional microorganisms and phosphorus dosages in soybean-maize and soybean-rice successions under no-till systems in the cerrado

2024-03-26T14:27:03-03:00

Multifunctional phosphate solubilizing microorganisms can contribute to reducing phosphorus doses without affecting the grain yield of crops. The aim of this work was to evaluate agricultural production systems involving soybean-maize and soybean-rice successions, inoculation of beneficial microorganisms and application of phosphorus doses with a view to sustainable intensification of agriculture and soil health and fertility in the Cerrados region. The experimental design was a randomized block design in a 2x4 factorial scheme with four replications. The treatments were composed of the combination of two phosphorus doses, 50% (45 kg ha^-1 of P₂O₅) and 100% (90 kg ha^-1 of P₂O₅) of the recommended dose with four uses of multifunctional microorganisms: 1. BRM 32111 (Burkholderia sp.), 2. BRM 32114 (Serratia marcescens), 3. co-inoculation (BRM 32111 + BRM 32114), and 4. control (no application of microorganisms). The microorganisms provided significant increases in the 100-grain weight and grain yield of soybeans, dry matter and nutrient accumulation of rice and maize, reduction of phytopathogenic fungus propagules, and increased accumulation of nutrients and activity of the enzymes Betaglicosidase and Arilsulfatase in the soil. Applying 50% phosphorus reduced the 100-grain weight and grain yield of soybean, dry matter and nutrient accumulation of rice, propagules of Trichoderma spp., and the nutrients in the soil. The soybean-maize succession showed higher levels of Arylsulfatase than the soybean-rice succession. The highest soybean yields were obtained by applying BRM 32114 with 50% and co-inoculation with 100% phosphorus.

Determination of biotechnological potential of poultry litter isolated bacteria

2024-02-28T18:31:22-03:00

Poultry litter is composed of residues from industrial wood processing, serving as a bed for the animals. It has also been widely used as an agricultural biofertilizer, increasing its economic importance. Few studies report the microbiological composition of this material or its biotechnological potential, which was the objective of this study. The microbiological composition was obtained by 16sRNA genetic characterization, as well as, phosphate solubilization capacity, indole-3-acetic acid (IAA) production, fungal antagonism, and enzymatic capacity. As a result, 67 isolates were obtained, of which 30 were taxonomically positioned and 27 were biochemically characterized. The microbiota genera consisted of Staphylococcus (17%), Brevibacillus (27%), Bacillus (37%), Mammaliicoccus (7%), Isoptericola, Microbacterium, and Oxalicibacterium (3.5% each). None of the isolates presented phosphate solubilization capacity. Regarding IAA production, UFPRCA19 showed the highest IAA content (16.60 µg.mg^-1protein). Eleven isolates were positive for antagonism with Aspergillus spp. and 25 for Trichoderma spp. Activity of proteases, caseinases, lipases, amylases, and cellulases were observed with the relative frequencies of 59%, 29%, 11%, 32%, 18%, and 21%. IAA and enzyme-producing bacteria as well as fungal antagonism were found among the isolates. Among all the isolated genera, Bacillus for synthesizing IAA and commercial enzymes becomes an interesting source for biotechnological purposes.

Pomegranate (Punica granatum L.) growth and biochemical alterations in response to meloidogyne incognita infection, minerals, and nano-fertilizers

2024-04-01T17:22:11-03:00

The effects of four inoculum levels (500, 1,000, 2,000, or 4,000 second-stage juveniles (J2) per plant) of the root-knot nematode, Meloidogyne incognita, on two pomegranate cultivars (‘Manfalouty’ and ‘Wonderful’) were investigated under greenhouse conditions in response to plant inorganic and organic chemical concentrations. Furthermore, the effects of six commercial chemical fertilizers (inorganic fertilizers and nano-fertilizers) on plant growth and nematode reproduction were also studied. Both cultivars recorded the highest gall formation, embedded stages, and final nematode population at the inoculum dosage of 2,000 J2/plant. The highest reproductive rate was achieved with 500 J2/plant, while the lowest rate was observed with 4,000 J2/plant on both cultivars. ‘Wonderful’, at all M. incognita inoculum levels, was more sensitive to nematode infestation than ‘Manfalouty’. ‘Wonderful’ showed a greater reduction in fresh and dry plant weights than ‘Manfalouty’ at 2,000 and 4,000 J2/plant. In both cultivars, the concentrations of N, P, and K decreased with increasing nematode inoculum levels. This decline was more pronounced in ‘Manfalouty’ than in ‘Wonderful’. The nano-fertilizers, Hyper Feed®, and Hyper Feed Solo® reduced all nematode parameters but only in ‘Wonderful’. Treatment with Hyper Feed® resulted in the highest percentage increases in all plant growth parameters in ‘Wonderful’. Total carbohydrate concentration was increased in ‘Manfalouty’ treated with the nano-fertilizers, especially with Hyper Feed®. Also, concentrations of total phenols and tannins increased in ‘Wonderful’ when treated with either nano-fertilizer. Generally, both nano-fertilizers showed an increase in plant N levels. We recommend using nano-fertilizers in integrated pest management (IPM) programs on pomegranate where they improved plant growth parameters and reduced nematode multiplication parameters more markedly than inorganic fertilizers.

Efficiency of three indigenous species of coccinellid predators for controlling aphids and whiteflies on cucumbers in greenhouses

2024-02-19T10:38:33-03:00

Cucumber plants can be infested with many insect pests such as aphids, whiteflies, and thrips, especially in greenhouses, resulting in significant damage to the crops. This study investigated the efficacy of three species of coccinellids (Coccinella undecimpunctata, Coccinella septempunctata, and Hippodamia variegata), indigenous to the Taif region of Saudi Arabia, to control Bemisia tabaci whiteflies and Aphis gossypii aphids on cucumbers in greenhouses. The study was conducted with a release rate of five second-instar larvae of each coccinellid species per plant. One week after the first release of coccinellids, there were no significant differences among the treatment groups in terms of infestation rate, but all three groups differed significantly from the control group. After the first week, the B. tabaci and A. gossypii infestation rates decreased significantly in the H. variegata group compared with the other two coccinellid species, a trend that continued until the end of the experiment. These results suggested that H. variegata was the most effective of the three species at decreasing the B. tabaci population. After the second release of each coccinellid species, the reduction rates were >90% in all three groups, being significantly higher in the H. variegata group. Thus, these results suggest that second-instar larvae of all three coccinellid species would be effective predators of B. tabaci and A. gossypii on cucumbers in greenhouses, with those of H. variegata showing the greatest efficacy. Future research should focus on the effects of these predators on outdoor cucumber fields and on other crops grown in the Taif region.

Honey consumer's perception: are brazilian consumers familiar with stingless bee honey?

2024-02-13T11:58:47-03:00

Stingless bees have a fundamental important role in the economies and traditions of South America indigenous cultures. Are mainly responsible for the pollination of several plant species. This way, the aim was to identify if Brazilian consumers know stingless bee honey and its sensorial characteristics, what is the consumption of this, as well as to generate information to assist in the development of the productive chain of the specie. A closed structured questionnaire with 20 questions was used and the participants were invited via social media. Data were collected in November 2020. The statistical analyses were performed related to the description, characterization of the participants and frequencies. In addition to these,, multivariate analyzes, for word association and questionnaire responses were performed. It is clear that most participants have some confusion between the sensory and physical characteristics of honey from native stingless bees and Apis mellifera. This lack of knowledge of the product can cause problems both in the handling of honey for sale and for consumption. Another interference would be linked to the taste, when we highlight the acidity and less sweetness of this pot-honey, and the consumer can often deduce that the product is damaged or not from a good origin. There is little consumer awareness of the benefits and qualities of stingless honey. Many consumers are still unaware of the differences between breeding sites and characteristics of native stingless bees. Still, a large part of these associates the quality of this honey with the honey of Apis Mellifera.

Effects of alternative fertilization on common bean crops regarding productivity and nutritional levels

2023-05-24T16:49:21-03:00

Considering the need for more information about productivity increases and nutritional improvement of soils, the present study evaluated the effects of alternative fertilization on grain yield and nutritional levels of bean crops. It was a randomized block study with four treatments and five replicates. The treatments were the absence of nitrogen fertilization, fertilizer use, chicken litter, and cattle manure. Organic fertilization provided lower variations in soil water availability during flowering and grain filling and increased nutrient concentrations, especially phosphorus and potassium. Chicken litter promoted the highest means for the number of legumes, the number of legumes on branches, the number of six-grain legumes, the mass of a thousand grains, grain mass per plant, and the normalized green-red difference index. However, the two organic fertilizers had significantly higher differences from chemical and no fertilization, showing that organic fertilizer applications must occur when nutrient availability coincides with the phenological stages essential for producing these fertilizers. High yields combined with favorable rainfall conditions occurred during crop development, as high soil moisture allowed faster mineralization of essential organic fertilizer nutrients, directly affecting yield. Pearson’s linear correlation allowed a better understanding of the participation of each plant trait in productivity, and the highest grain yield occurred with organic fertilization.

Development of nicotiana-specific molecular markers and their application in a loop-mediated isothermal amplification assay

2023-06-01T19:45:58-03:00

An effective identification method for detecting illegal goods involving raw tobacco material is crucial for tobacco monopolies to conduct surveillance. We developed Nicotiana-specific molecular markers to determine whether seized goods contain raw tobacco material. The sequence data for genes related to the nicotine metabolism pathway and genomic data from the public Solanaceae database were used to establish Nicotiana-specific molecular markers. These markers were determined by experimentally verifying 17 types of nontobacco plant material and 91 types of tobacco material belonging to 11 sections of 3 subgenera. Two reliable Nicotiana-specific markers, Ntsp027 and Ntsp151, were selected from among the 209 newly developed markers. The results indicated that the primers corresponding to these two markers can amplify the target fragments in the 91 types of Nicotiana material without amplification of any PCR products in the 17 types of non-Nicotiana material. Furthermore, utilizing the marker Ntsp151, we verified the efficacy of the loop-mediated isothermal amplification (LAMP) assay in authenticating tobacco material. The identification of 21 tea-cigarette products via the combination of GC‒MS, a Nicotiana-specific molecular marker and LAMP methods underscores the utility of Nicotiana-specific DNA markers in determining whether illegal goods contain raw tobacco material. Our results indicate an impressive accuracy rate of 100%, which is consistent with the reliability assessment, underscoring the accuracy of these markers in effectively identifying tobacco material. Our findings can significantly augment the capacity for surveillance and anticounterfeiting efforts by aiding the fight against illicit trade and ensuring the integrity of all tobacco-related products in the market.

Alternative products for controlling Sclerotinia sclerotiorum in vivo AND in vitro in canola crops

2023-06-07T18:55:42-03:00

Products that activate plant defense mechanisms, such as white mold, may increase the disease control spectrum in canola crops as an alternative to chemical control for disease management, aiming at lower environmental impacts and selection pressure on pathogens. This study evaluated Sclerotinia sclerotiorum control and resistance induction in canola crops with alternative products. In vitro and detached leaf experiments were conducted in a laboratory, and in vivo experiments occurred in a greenhouse. High Roots®; V6®; Maxi Flor®; Wert Plus®; potassium, manganese, and copper phosphites; manganese; procymidone fungicide; and Ascophyllum nodosum, Bacillus subtilis, and Bacillus thuringiensis extracts assessed S. sclerotiorum mycelial growth in vitro in detached leaves, white mold severity in a greenhouse, and enzymatic analysis in leaf tissues. The last two experiments also received the acibenzolar-S-methyl and Trichoderma asperellum treatments. S. sclerotiorum mycelial growth showed 100% inhibition under B. subtilis and Wert Plus® at 1000 ppm and procymidone at 100 and 1000 ppm in vitro. The procymidone and B. subtilis treatments in detached leaves inhibited 100% of fungus growth. The greenhouse experiments with lower severity were procymidone, copper phosphite, B. thuringiensis, B. subtilis, manganese phosphite, potassium phosphite, High Roots®, and V6®. Higher peroxidase enzyme activity occurred in plants treated with acibenzolar-S-methyl, copper phosphite, and fungicide 48 hours after pathogen inoculation. The polyphenol oxidase enzyme did not show activity. Alternative products were responsive and may aid the chemical control of white mold in canola crops.

Proposal for a new non-linear model to describe growth curves

2023-09-28T07:39:52-03:00

This study was developed with longitudinal data measurements of Norfolk rabbits from birth to 119 days of age to estimate the average growth curve, with the primary objective of proposing a non-linear model. It also selected the most appropriate sigmoidal model to describe the growth of Norfolk rabbits. The adjustments provided by the logistic, von Bertalanffy, Gompertz, Brody, Richards, and proposed models were compared. The parameters were estimated using the “nls” function of the “stats” package in R software, the least-squares method, and the Gauss-Newton convergence algorithm. The goodness-of-fit comparison was based on the following criteria: adjusted coefficient of determination (), mean square error (MSE), mean absolute deviation (MAD), Akaike information criterion (AIC), and Bayesian information criterion (BIC). Cluster analysis helped select and classify the non-linear growth models, considering the other goodness-of-fit criteria results. The proposed non-linear, von Bertalanffy, Gompertz, and Richards models described the growth curve of Norfolk rabbits satisfactorily, providing parameters with practical interpretations. The goodness-of-fit criteria showed that the proposed and von Bertalanffy models best represented the growth of rabbits.

Artificial neural network model for water consumption prediction in dairy farms

2023-10-30T15:01:26-03:00

This work presents a model based on artificial neural network (ANN) applied to predict water consumption in Brazilian dairy farms. Inputs were simple process data such as number of lactating cows, milk productivity, type of management, among others, with low computational cost and satisfactory data prediction. Data used for ANN training was acquired during two years from 31 farms in semi-confined dairy production. The analysis of the results was based on the following statistical models’ indicators: R² (Coefficient of determination), BIAS (trend coefficient), MAE (mean absolute error), RMSE (Root-mean-square deviation), NRMSE (percentage of the mean of the observations) and RAE (Relative absolute error). After performing the ANN training, the results showed good accuracy to predict water consumption in Brazilian dairy farms, with an average absolute error of 28.4% being obtained. On the other hand, considering the dataset used for ANN validation, an average absolute error of 48% was obtained.

Comparison of pure caucasian, carniolan and native anatolian ecotype honey bee (Apis mellifera L.) colonies in the eastern anatolia region with reciprocal F1 hybrids

2023-09-28T07:54:52-03:00

The aim of this study was to compare specific physiological and behavioral characteristics of A. m. caucasica, A. m. carnica, and native honeybees, as well as reciprocal crosses, within the ecological conditions of East Anatolia (Van province), Turkey. The findings revealed that native honeybees exhibited the highest average sealed brood area and the highest average number of frames with bees. In contrast, the highest average honey yield was observed in the crossbreeding of Carniolan bees with native bees. Significant differences were identified among the groups concerning brood area, the number of bee frames, aggression tendency, and honey yield. The study concluded that Carniolan and Caucasian bees exhibited suboptimal performance outside their native habitats. Nevertheless, Carniolan bees were identified as a viable option for mitigating aggression tendencies and enhancing honey yield in native bee populations.

A meta-analysis of physicochemical changes in the rhizosphere and bulk soil under woodlands

2024-01-03T12:09:52-03:00

Monoculture for timber production has been replacing natural environments as the demand for renewable energy sources increases. The lack of nutrient compensation may increase the risk of soil depletion, thus changing soil properties. To summarize the impact of forestry activities in edaphic environments, we present a meta-analysis on the rhizosphere effects of coniferous and broadleaved trees established as monoculture and natural regeneration on soil physicochemical properties. Records of soil attributes published in peer-reviewed journals from eight countries were collected. Clay content changed only in monoculture sites, decreasing 55.51% in the rhizosphere, while silt and sand presented significant variations in both monoculture and naturally regenerated areas. Conifers affected the soil more than broadleaved trees, evidenced by higher pH reduction (-2.96% vs. -1.98%) and higher increase of Al³⁺ (197.43% vs. 50.68%), K⁺ (80.40% vs. 69.90%), CEC (24.61% vs. 17.35%), and total organic carbon (82.21% vs. 69.89%). Also, the rhizosphere affected regeneration soils more than monoculture, indicated by higher Al³⁺ (50.68% vs. ns) and available P (32.31% vs. ns), K⁺ (203.44% vs. ns), CEC (34.90% vs. 20.93), and total organic carbon (91.55% vs. 63.23%). These results indicate higher nutrient availability in naturally regenerated than monoculture sites, as higher species diversity and better plant litter quality are expected. This meta-analysis shows that coniferous and naturally regenerated trees had a higher influence on the rhizosphere and soil properties than broadleaved and monocultures. Management practices must be revisited to ensure the long-term sustainability of forestry activity, and studies in tropical zones must be intensified.

Increased efficiency fertilizers in arabica coffee growth in Vale do Ribeira, SP

2023-12-08T17:09:11-03:00

Controlled-release fertilizers have been increasingly used. This study aimed to evaluate and adapt new technologies applied via soil for sustainable coffee production, in order to generate information that contribute to the technical innovation of the crop for the Vale do Ribeira region. The experiment was set at UNESP, in Registro – SP. The experimental design was in randomized blocks. The experiment consisted of eight treatments with four replications, with plots of six plants. Four doses (200, 300, 400 and 500 kg ha^-1) of a mixed fertilizer 20-05-20 were used, with controlled release in six months, intended for coffee trees in formation and production, compared to the dose of 500 kg ha^-1 of the conventional mixed fertilizer 20-05-20, ammonium sulfate and calcium nitrate with boron, in addition to a control treatment, which did not receive NPK fertilization. The cultivar used was ‘Obatã IAC 1669’ in 3.0 x 0.6 m spacing. The following characteristics were evaluated: number of plagiotropic branches, number of nodes of plagiotropic branches, stem diameter, plant height and yield, in two harvest periods, besides the surface chemical characteristic of the soil. Increasing the dose of the slow-release fertilizer leads to greater plant growth; the coffee plant presents a highly responsive behavior to the increase in fertilizer doses in relation to nitrogen, and the use of the slow-release fertilizer Agroblen (20-05-20) 100% and ammonium sulfate + SS + KCl allows greater yield.

Schizolobium parahyba var. amazonicum seedling growth by Trichoderma spp. strains under nitrogen rates

2023-05-23T17:20:42-03:00

This study evaluated the initial development of paricá (Schizolobium parahyba var. amazonicum) seedlings under different nitrogen rates with the application of Trichoderma spp., using a randomized complete block design in a 4x5 factorial scheme (strains and rates) with seven replications. The evaluated traits were plant height, stem diameter, leaf and stem fresh weights, leaf and stem dry weights, and aerial part dry and fresh weights. Trichoderma spp. strains did not satisfactorily promote paricá seedlings (Schizolobium parahyba var. amazonicum) under high nitrogen rates. However, the Trichoderma harzianum IBLF 006 WP strain was efficient only under low nitrogen availability.

Relationship of potassium doses with bioethanol yield in sweet potato in Cerrado soil

2023-05-23T17:13:57-03:00

Sweet potato-bioethanol yield was evaluated in response to potassium fertilizer application. Experiments were performed using a 5 × 2 factorial design in which factors included the amount of K₂O applied to the soil, with five levels (0, 30, 60, 120, and 240 kg ha^-1) and genotype, with two levels (industrial genotype BDGPI #25 and table genotype BDGPM #04). Root yield, root starch and soluble solid contents, bioethanol yield, and economic viability of potassium application for bioethanol production were evaluated. Potassium affected root yield of both genotypes, with the highest yield observed at 140 kg K₂O ha^-1. Root starch concentration at harvest depended on genotype potential rather than potassium dose. Soluble solid content in fresh roots was lower than that in cooked roots, in which case, maximum conversion efficiency was observed at 109,69 and at 123.75 kg K₂O ha^-1 for BDGPM#04 and BDGPI#25, respectively. Bioethanol yield reached 10,484 and 9,839 L ha^-1 at 151.87 and 136 kg K₂O ha^-1 for BDGPI#25 and BDGPM#04, respectively. Genotype BDGPI#25 was more efficient than sugarcane in converting potassium to bioethanol at 151.87 kg K₂O ha^-1, producing 10,484.29 L of bioethanol. In turn, BGDPM#04 showed maximum conversion efficiency relative to sugarcane at 122 kg K₂O ha^-1.

Effect of glycerol monolaurate nanocapsules on Streptococcus mutans biofilm in vitro

2024-08-06T16:43:49-03:00

This study aimed to prepare and apply nanocapsules containing glycerol monolaurate for eradicating Streptococcus mutans biofilms. The interfacial deposition method of the preformed polymer synthesized the nanocapsules characterized for mean diameter, polydispersity index, zeta potential, pH, and morphology by transmission electron microscopy. The microdilution method investigated antimicrobial activity. Crystal violet staining determined biomass quantification and the ability to inhibit biofilm formation. The study also measured exopolysaccharide production and the number of viable colonies. The characterization outcomes indicated acceptable values for the mean diameter 198.1 ± 2, a polydispersity index of 0.087 ± 0.018, a zeta potential of -21.30 ± 2.00 mV, a pH of 6.19 ± 0.12, and typical nanostructure morphology. The evaluations of minimum inhibitory and bactericidal concentrations of glycerol monolaurate (free and nanoencapsulated) revealed their ineffectiveness in inhibiting microorganisms. Only free glycerol monolaurate inhibits S. mutans growth with 125 µg/mL. Biomass, exopolysaccharide content, and viable colonies in the biofilm were analyzed to assess the compounds' ability to inhibit biofilm formation. The tested compounds did not significantly reduce the formed biofilm. Despite unfavorable outcomes of the formulated preparation, further experimentation with a new formulation is encouraged to explore alternative strategies and potential improvements.

Study of acute and subacute toxicities and genotoxic and mutagenic potentials of the lyophilized extract of Campomanesia sessiliflora (O.Berg) mattos leaves in wistar rats

2024-06-05T14:38:29-03:00

Campomanesia sessiliflora (O.Berg) Mattos is a Brazilian native plant species used in a popular medicinal tea for treating gastrointestinal, urinary, and dermatological pathologies. This study evaluated the toxicity of Campomanesia sessiliflora (O.Berg) Mattos via acute and subacute toxicity tests. It also analyzed mutagenic and genotoxic potentials by the micronucleus test, which detects genetic material damage indicating mutagenicity, and the comet assay, which assesses DNA damage levels as a genotoxicity indicator. The plant extract initially originated from the ultrasonic maceration of Campomanesia sessiliflora (O.Berg) Mattos leaves in a hydroethanolic solution. The involved animals were adult Wistar rats. Ten females were available to evaluate acute toxicity and estimate the LD50, receiving a dose of 2000 mg/kg. The subacute toxicity evaluation used 35 females and 35 males divided into seven groups: negative control (saline control – SC), positive control (cyclophosphamide control – CC), 125 mg/kg (125), 250 mg/kg (250), 500 mg/kg (500), 1000 mg/kg (1000), and the satellite group (ST). Genotoxicity and mutagenicity experiments applied bone marrow micronucleus and comet assays. Acute and subacute toxicity tests did not present behavioral, physical, and physiological changes (p≥0.05). Administering the Campomanesia sessiliflora (O.Berg) Mattos extract reduced spleen size in male and female animals, without histopathological changes. However, doses above 500 mg/kg showed significant genotoxic and mutagenic effects in the comet and micronucleus assays compared to the control group. The extract did not exhibit acute or subacute toxicity, but doses higher than 500 mg/kg indicated some level of genotoxicity and mutagenicity.

In vitro photoautotrophic cultivation of Melocactus zehntneri: insights from allometric characteristics from germination to acclimatization

2024-10-01T15:20:57-03:00

Melocactus zehntneri is a species of ornamental relevance suffering anthropogenic pressures, putting its preservation at risk. Thus, alternatives for propagating and conserving this species are needed. This study evaluated the effect of photoautotrophic cultivation of M. zehntneri to produce plants well acclimated to natural conditions. The seeds underwent different imbibition times before in vitro germination. Besides the growth room, a greenhouse hosted the photoautotrophic cultivation of seedlings. Vermiculite replaced agar in the culture medium. The imbibition pre-treatment in distilled water and the growth environments affected some parameters linked to germination. The greenhouse provided better characteristics for plants grown in vitro and for acclimatization than those kept in the growth room. Plants cultivated and acclimated ex vitro in a greenhouse photoautotrophic environment showed improved characteristics regarding ornamental significance.

Synthesis and evaluation of the antimicrobial activity of purified and unpurified multi-walled carbon nanotubes

2024-06-13T19:27:06-03:00

Multi-walled carbon nanotubes (MWCNTs) were purified and unpurified in this study to obtain hybrid materials with improved activity. The production stage comprised a chemical purification of the produced sample. Raman spectroscopy analyzed the structural composition of purified and unpurified samples. The disc diffusion assay, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and time-kill assessment analyzed antimicrobial activity. MWCNT performed well against the tested bacteria (Klebsiella pneumoniae, Pseudomonas aeruginosa, Enterobacter aerogenes, Staphylococcus aureus, and Streptococcus sp. Staphylococcus epidermidis). The disc diffusion assay revealed inhibition zone differences caused by purified and unpurified MWCNTs. MIC and MBC values of purified and unpurified MWCNTs were similar. The purified and unpurified nanotubes of Staphylococcus epidermidis (ATCC 35985) exhibited inhibition zone diameters of approximately 8 mm and 9 mm, respectively. The microdilution method revealed a MIC of 1.23 mg/ml for the purified nanotube and 0.156 mg/ml for the unpurified nanotube against the same microorganism. The killing curve analysis demonstrated that unpurified carbon nanotubes were more effective against all tested microorganisms. MWCNTs represent a promising method for microbiology, but studies on the toxicity of these materials remain scarce.

Evaluation of behavioral responses to restraint stress in the house mouse (Mus musculus musculus) of wild origin

2024-10-01T15:57:20-03:00

Stress is a fundamental concept for many areas of animal research. In laboratory rodents, exposure to various stressors results in immediate and postponed behavioral changes that can be measured with standard tests. Less is known about how wild rodents that have not undergone domestication respond to stressors in laboratory settings. This study was aimed at evaluating behavioral responses to restraint stress in male and female wild-derived house mice (Mus musculus musculus). One week after the end of three daily restraint sessions, alterations in mouse behavior were assessed in two tests. In the open field test, stressed mice (n = 23) entered the unprotected central zone less frequently (P < 0.05) and showed increased duration of self-grooming (P < 0.05) compared to controls (n = 23), indicating elevated anxiety. In the forced swim test, most mice (44 out of 46) displayed episodes of behavioral despair, but the influence of stress was insignificant. To characterize baseline performance of wild-derived mice, their behavior was compared to that of a laboratory strain. As compared to C57BL/6 males (n = 10), M. m. musculus males (n = 14) showed significantly greater exploratory activity in the open field and longer latency of the first immobility episode in the forced swim test (P < 0.001). Overall, these findings confirm ecological validity of the widely used animal model of restraint stress and may serve as a basis for future studies.

Dodecanoic acid induces oxidative stress-mediated death in liver cancer cells through the mitochondrial pathway

2024-02-09T15:47:03-03:00

It has been reported that dodecanoic acid (DDA) exerts anticancer effects on cancers of the reproductive system and digestive system. However, its role in liver cancer and its potential mechanism have rarely been defined. Therefore, in this study, Hepa 1-6 liver cancer cells were incubated with different DDA concentrations (0.1, 0.3, 0.5, 1, 2, 4 mM) for 24, 48 and 72h, and the optimal DDA concentration was determined via a cell viability test. Apoptosis and cell cycle distribution were determined by flow cytometry. SOD activity, mitochondrial membrane potential (MMP), ATP, GSH and ROS levels were measured by commercial assay kits; Bcl-2, Bax and Caspase-3 protein levels were analyzed by western blot. The results showed that 0.5 mM DDA decreased cell viability in a time-dependent manner, so this concentration was used to investigate how DDA leads to Hepa 1-6 cell apoptosis. After treatment with DDA, a significant, time-dependent increase in the cell apoptotic rate was detected despite the accumulation of S-phase cells. The increased ROS levels and decreased GSH levels and SOD activity in DDA-treated cells indicated the occurrence of oxidative stress. Mitochondrial dysfunction was evidenced by a decreased MMP and reduced ATP levels. Cell apoptotic death via the mitochondrial pathway was indicated by a reduced Bcl-2/Bax ratio and increased caspase-3 protein levels. It can be concluded that DDA can effectively trigger liver cancer cell death by inducing oxidative stress and disrupting mitochondrial function. These findings provide new insight into the potential mechanism of action of DDA in liver cancer.

Study on extraction conditions and characteristics of protein from burdock roots

2024-03-14T13:30:34-03:00

Burdock (Arctium lappa L.), which belongs to the Compositae family, possesses significant medicinal and nutritional value. Burdock root is abundant in proteins and essential amino acids. In this study, proteins from burdock roots were extracted by alkali extraction and acid sedimentation, and the optimal conditions for protein extraction from burdock roots and protein characteristics were explored. The optimal conditions for protein extraction with a yield rate of 9.03% were 40°C extraction temperature, pH 8.0, a solid–liquid ratio of 1:25 (g/mL), and extraction time of 65 minutes. Single-factor and orthogonal tests on the extraction rate of burdock proteins under different conditions found that the extraction rate of protein from burdock roots were influenced by pH, extraction temperature, solid–liquid ratio, and extraction time, in this order. The solubility and gelation properties of burdock protein were similar to those of soybean protein; however, burdock protein demonstrated 1.2 times the oil absorption capacity and half the water retention capacity of soybean protein. Analysis of the amino acid contents of burdock protein revealed that burdock root contains significant essential and non-essential amino acids, with valine content being the highest.

Diversity in pollen grain characteristics and its importance in distinguishing Loranthaceae Juss. species grown in Saudi Arabia

2023-12-27T10:21:37-03:00

Members of the Loranthaceae family are considered parasitic, and mistletoe negatively affects the growth and productivity of host trees. Loranthaceae has several complicated taxonomic issues. Six species of Loranthaceae grow naturally in Saudi Arabia, but these species have not been well studied. The aim of this work was to evaluate the importance of morpho-palynological characters as a taxonomic tool in the identification of Loranthaceae species growing in Saudi Arabia. The discriminatory power of these characteristics was evaluated using different statistical analysis methods. The descriptive characteristics of pollen polar and equatorial views and colpus type are useful for describing the species, and the presence of three colpus patterns was detected among the study samples: syn-(3)-, demisyn-(3)-, and zono-(3)-colpate. The quantitative characteristics, specifically, the equatorial diameter and P/E ratio, are the most useful and can be used to classify species within clusters based on their affiliation with genera, species, and subtribes Emelianthinae and Tapinanthinae. This study confirmed that pollen grain characteristics can be used as a taxonomical tool to identify Loranthaceae species. This information will aid in determining parasitic species that infect trees and could be useful for strengthening efforts in weed management.

Fungal profile of eggshells from commercial and free-range hens of a supermarket chain in Fortaleza, CE, Brazil

2024-03-02T19:58:58-03:00

Eggs are perishable and fragile foods because of their easy contamination by different microorganisms, such as fungi. Hence, research on egg safety and quality has been recently developed worldwide to minimize illness transmitted by this food, although most present mainly pathogenic bacteria. Thus, this study identified potentially pathogenic fungal species in eggshells from free-range and commercial hens offered in supermarkets in Fortaleza, CE, Brazil. The research was performed at the Vettings laboratory at the State University of Ceará (UECE) with 40 eggs: 20 from commercial and 20 from free-range hens. The eggshells were assessed using the dextrose evaluation method for pathogenic cultivation, commensal, and yeast fungi. Subsequently, readings were taken to identify and count the fungi on a binocular light microscope in 100x increments. Both egg categories showed a significant number of colony-forming units (CFU). The study identified eight different fungal species in both egg production types. The most prevalent fungal colonies in the free-range hen lineage (brown shell) were yeast spp. and Penicillium citrinum, and the laying hen lineage (white shell) had mostly Penicillium spp. and yeast spp. Therefore, both egg production systems showed similar fungal contamination, which suggests that the manipulation and/or storage are adequate despite the lack of strict food safety regulations or supervision for eggs in Brazil.

Investigation of the mutagenic, cytotoxic and antimicrobial effect of bikaverin mycotoxin

2024-01-30T18:12:30-03:00

Bikaverin is a reddish pigment produced by different fungi species (Mycogone jaapii, Verticillium agaricinum, Beauveria bassiana, Paecilomyces fumosoroseus, Polyporus sulphureus), mainly of the Fusarium genus. Due to its pigment feature, bikaverin can be used as a dye in various fields in the industry. However, it is extremely important to study the mutagenic/genotoxic effects, cytotoxic effects and antimicrobial properties of bikaverin for application of industrial areas. In the study, the mutagenic, cytotoxic and antimicrobial effects of bikaverin were investigated. The mutagenic effect of bikaverin was studied with the Ames test. Salmonella typhimurium TA97a, TA98, TA100, TA102 and TA1535 strains were used in the test. Five different doses of bikaverin (0.075, 0.1, 0.2, 0.3 and 0.5 μg/plate) were tested against strains. It was determined that there was no mutagenic effect of bikaverin. The cytotoxicity of bikaverin was evaluated by MTT test on L929 fibroblast cell line. Bikaverin demonstrated no cytotoxic effect on L929 fibroblast cell line, according to cell viability calculations that showed >73% for all concentrations (1, 0.5, 0.4, 0.3, 0.2, 0.1, 0.075, 0.05, 0.025, 0.01, 0.005 and 0.001 μg/mL) examined. Bikaverin's IC50 value was determined to be 1.79±0.51 g/mL. The antimicrobial activity of the bikaverin was evaluated by using the microdilution method. Bikaverin was found to have antimicrobial effects on Methicillin resistant Staphylococcus aureus, Vancomycin resistant Enterococcus faecalis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Candida albicans and Candida krusei, as MIC values ranged from 1.25 -5 µg/ mL.

Spray drying of cellulases produced by Bacillus sp. SMIA-2

2023-09-07T18:48:09-03:00

Microbial cellulases present biotechnological potential to be used in several industries, including food, brewery and wine, industrial waste for chemical feedstock, animal feed, pulp and paper, agriculture, textile and detergent production. In this work, cellulases produced by the thermophilic Bacillus sp. SMIA-2 in submerged cultures containing sugarcane bagasse, corn steep liquor and passion fruit rind flour were spray-dried, in an attempt to improve their stability for industrial purposes. The cellulases were spray dried and analyzed before and after the drying process and subsequent storage. A Central Composite Design (CCD) 2³ was used to investigate the effect of different concentrations of arabic gum and microcrystalline cellulose, as well as the spray dryer inlet temperature on the cellulase spray drying process. The results evidenced that the combination of 1.0 % (w/v) arabic gum and 1.0% (w/v) microcrystalline cellulose, at inlet temperature of 70 °C, was effective in maintaining the activities of both avicelases (avicel-hydrolyzing enzymes) and carboxymethylcellulases (carboxymethylcellulose-hydrolyzing enzymes - CMCase). The dried avicelase was completely preserved when stored at 5^oC, while the CMCase retained 89% of its activity, which indicates promising potential for industrial uses, especially in detergent formulations.

Kaempferol promotes wound-healing in diabetic rats through antibacterial and antioxidant effects, devoid of proliferative action

2023-06-23T17:55:30-03:00

The investigation of novel phytochemicals for the prevention and treatment of infections caused by multidrug-resistant pathogens is gaining attention. The current study evaluated the in-vitro antimicrobial activity of kaempferol against methicillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa. Its in-vivo efficacy in inhibiting these pathogens was determined using an excision wound model in nicotinamide-streptozocin- -induced diabetic rats. Kaempferol displayed an inhibitory effect against the tested bacteria both in vitro and in vivo. It also healed excision wounds at a 1% (w/w) concentration. An increase in antioxidant enzymes in wounded tissue was observed on kaempferol treatment. A reduction in the MRSA and P. aeruginosa counts in wounded tissue together with a reduced epithelization period was observed when compared to the infected control. A thicker epithelium, new capillaries, and a decrease in inflammatory cells were detected by hematoxylin and eosin staining. Furthermore, an increase in collagen fibers and their deposition was observed by Masson’s trichrome staining. Kaempferol at 40 µM did not display any toxicity for human keratinocytes grown in media containing high glucose and it did not affect the expression of the pro-healing cytokines genes vascular endothelial growth factor (VEGF) and transforming growth factor-b-1 (TGFβ1). Kaempferol displayed antibacterial and antioxidant actions but did not increase the expression of proliferative genes.

Investigation of crude methanolic extract from poison secreted by the Rhaebo gutattus on status redox antioxidant in mice

2023-09-20T10:52:35-03:00

The study evaluated the antioxidant properties of a crude methanolic extract (CME) from Rhaebo guttatus poison in mice over a period of 7 and 30 days. The mice were divided into groups and treated with different concentrations of the extract (0; 8 μg mL^-1; 16 μg m^L-1 and 32 μg mL-1 or vehicle; 100 μL/animal/day; via gavage). The liver samples were analyzed for status redox parameters as catalase (CAT), glutathione-S-transferase (GST), reduced glutathione (GSH) and thiobarbituric acid reactive substances (TBARS). The results showed that the CME caused changes in the levels of various antioxidants and oxidative stress markers. At 7 days, there was an increase in TBARS levels (8 μg mL^-1 dose) and GST activity (16 μg mL^-1 dose), and a reduction in GSH levels (32 μg mL^-1 dose) compared to the control group. At 30 days, TBARS and GSH levels returned to control values in the same period, but GSH increased (32 μg mL^-1 dose) compared at 7 days; GST activity remained high after 30 days for 32 μg mL^-1 dose compared other groups and time of treatment (7 days). Overall, the study suggests that the extract modulates antioxidant properties per se that can affect various markers of status redox in the liver of mice, mainly 16 μg mL^-1 dose demonstrated to act under antioxidant enzymes in different times (7 or 30 days).

Effect of mite-specific subcutaneous immunotherapy on patients with allergic rhinitis

2024-06-17T14:58:36-03:00

This study analyzed the effect of mite-specific subcutaneous immunotherapy (SCIT) on patients with allergic rhinitis (AR). We enrolled 98 AR patients visiting our hospital from April 2017 to April 2019 and grouped them in a random number table. The control group (n=49) received conventional treatment for three years. The SCIT used a standardized mite allergen injection for the experimental group (n=49) for three years. The study compared total nasal symptom score (TNSS), daily medication score (DMS), total combined score (TCS), visual analog scale (VAS) score, mini-rhinitis quality of life questionnaire (MiniRQLQ) score, and serum immunoglobulin E (sIgE) level before and after treatment. The overall response rate was higher in the experimental group than in the control group (59.18% vs. 30.61%, p<0.05). After treatment, the experimental group had lower values for TCS and VAS score (p<0.05); motion score; practical problems; nasal, ocular, and other symptoms (p<0.05); and sIgE, Dermatophagoides pteronyssinus (Dp)-sIgE, and Dermatophagoides farinae (Df)-sIgE levels (p<0.05) than the control group. The sIgE, Dp-sIgE, and Df-sIgE levels were lower in the effective group than in the ineffective group (p<0.05). The areas under the ROC curves of IgE, Dp-sIgE, and Df-sIgE and their combination for predicting the therapeutic effect of mite-specific SCIT on AR were 0.839, 0.779, 0.814, and 0.903, respectively. Mite-specific SCIT relieved clinical symptoms and improved the quality of life of AR patients, probably by decreasing the IgE expression level.

Increased resistance rates of extended-spectrum β-lactamase-producing Enterobacterales isolated from blood cultures in Brazil

2024-10-01T16:35:45-03:00

This study aimed to identify and evaluate the antimicrobial susceptibility profile of extended-spectrum β-lactamase (ESBL)-producing Enterobacterales isolated from blood cultures of patients with suspected sepsis. This retrospective cross-sectional epidemiological study was performed for four years (2015 to 2018) by accessing the medical records of patients diagnosed with bloodstream infections in two tertiary public hospitals in Brazil. Culture results and susceptibility tests of bacterial strains isolated from blood samples were analyzed. During four years, 2,263 blood culture exams were performed, and Enterobacterales were isolated in 694 (30.7%) samples. The disk approximation test detected ESBL production in 171 (24.6%) of the 694 isolated Enterobacterales (p<0.05), with 125 (73.1%) corresponding to Klebsiella spp., 28 (16.2%) to Escherichia coli, eight (4.6%) to Enterobacter spp., five (2.9%) to Serratia spp., three (1.7%) to Proteus spp., and two (1.2%) to Citrobacter spp. All tested antibiotics demonstrated low susceptibility (2.9% to 26.9%). The classes of monobactams, cephalosporins, and penicillin showed high resistance indices. The evaluated Enterobacterales isolates exhibited expressive antimicrobial resistance to drugs commonly used as empirical treatment in bloodstream infections. The findings reinforce the relevance of assessing the bacterial antimicrobial susceptibility profile, as it drives antibiotic therapy, potentially detecting multiresistance cases.

Impact of occlusal schemes on prognosis of conventional complete dentures in patients with severely resorbed alveolar ridges

2024-04-18T14:53:01-03:00

Bilateral Balanced Occlusion (BBO) and Canine Guidance (CG) are occlusal schemes that have been successfully used in complete dentures (CDs). The objective of this study was to evaluate the effects of CG compared to BBO on satisfaction, oral health-related quality of life (OHRQoL) and masticatory performance in CDs wearers with severe resorbed alveolar ridge by means of a crossover trial. Sixteen edentulous patients with severe resorbed ridges were treated with new maxillary and mandibular CDs. After adaptation period, they were randomly divided into two groups according to a sequence of occlusal schemes: BBO for 30 days followed by CG for 30 days or, CG for 30 days followed by BBO for 30 days. Patient satisfaction with their dentures, OHRQoL and masticatory performance were assessed immediately 30 days after of using each occlusal scheme. The results of satisfaction and quality of life were using Wilcoxon test, and data regarding masticatory performance were analyzed through paired t-test (α = .05). The occlusal scheme used in complete dentures had no significant influence on general patient satisfaction (p = 0.06), overall quality of life (p = 0.09), or masticatory performance of patients (p = 0.182). Both BBO and CG can be considered as suitable for oral rehabilitation with CDs in patients with severe resorbed alveolar ridges. However, CG may be considered as a scheme of choice by clinicians due to its greater simplicity and shorter execution time.

Monocyte-lymphocyte, neutrophil-lymphocyte, and platelet-lymphocyte ratios as inflammatory biomarkers of clinical dengue severity

2023-04-04T15:48:57-03:00

The literature describes monocyte-lymphocyte (MLR), neutrophil-lymphocyte (NLR), and platelet-lymphocyte (PLR) ratios as prognostic biomarkers. However, in the case of dengue infection, patient clinical management and nonspecific laboratory tests determine the prognosis. Therefore, this study analyzed MLR, NLR, and PLR as prognostic biomarkers of dengue infection. Our study was based on a clinical cohort of dengue patients in Brazil between 2012 and 2013. From 193 patients, 164 (85.0%) were classified as dengue fever (DF), 19 (9.8%) as dengue hemorrhagic fever (DHF), and 10 (5.2%) as intermediate DF/DHF. DHF cases were significantly associated with MLR > 0.13 (OR: 5.72, 95% CI: 1.28-25.60, p < 0.05) and PLR ≤ 80.68 (OR: 4.26; 95% CI 1.60-11.33; p < 0.05). Our results suggest that MLR increase, and PLR decrease indicate a higher likelihood of worsening the clinical status.

Infectious disease agent surveillance in fitness centers in northern cyprus: is methicillin-resistant Staphylococcus aureus (mrsa) infection a threat?

2024-03-28T19:11:41-03:00

Fitness centers offer an opportunity for investigating the indirect transmission of pathogens. Many people with varying levels of personal hygiene share sports equipment where direct surface-to-skin contact occurs. This study aimed to investigate the bacterial load and fungal and methicillin-resistant Staphylococcus aureus (MRSA) contamination on predetermined sports equipment in fitness centers in Northern Cyprus. Additionally, volunteer personal trainers and gym members were screened to detect whether they were carriers of MRSA. Samples were collected from six fitness centers on sports equipment. MRSA carrier status was investigated for personal trainers (n=10) and gym members (n=100). The study used culture-dependent techniques and used SPSS 20 software for statistical analysis. There was no statistically significant difference between fungal growth on the sports equipment, and Aspergillus spp. were predominant. However, one of the fitness centers had a statistically significant difference in fungal growth compared to the others (p<0.005). There was no significant difference in the bacterial load among the sports equipment, but there was a significant difference among the fitness centers (p<0.009). Among all tested individuals, only 2.7% (3/110) were MRSA-positive, all of whom were gym members. No MRSA was detected on any of the equipment. Despite the increasing incidence of community-acquired MRSA infections, the fitness centers in this study did not appear to be significant sources of staphylococcal or fungal infections. However, the detection of MRSA carriers among gym members suggests that the spread of MRSA between individuals in gyms is still possible.

Effect of Tai Chi on adult mental health during the pandemic and factors associated: a cross-sectional study

2024-03-22T14:57:39-03:00

This cross-sectional study aimed to assess whether levels of anxiety, perceived stress, and self-perception of happiness during the Covid-19 pandemic were lower among Tai Chi (TC) practitioners. An online questionnaire was applied from September 2020 to November 2021 through email list and instant messaging application "WhatsApp". In total 368 individuals, 341 of whom did not practice TC, coming from a university and sports academies and 27 TC practitioners from the Brazilian Society of TC and Oriental Culture were included. Sociodemographic, mental health, habits related to physical activity and the State Anxiety Inventory (STAI), Perceived Stress Scale (PSS14) and the Andrews Faces Scale were collected to assess level of state-anxiety, perceived-stress, and self-perception of happiness, respectively. Each independent variable with the outcome variables (severe anxiety level, severe stress level, moderate/severe stress level and self-perception of happiness) were analyzed for simple and multiple logistic regression model. Individuals who do not practice TC are more likely to have a severe level of anxiety (OR=3.55; CI95%CI: 1.37-9.20) and a moderate/severe level of stress (OR=2.60; 95%CI: 1.07-6.35), p<0.05. TC practitioners are more likely to perceive themselves as happy than non-practitioners (OR=3.18; 95%CI: 1.16-8.71), p<0.05. Do not practice regular physical activity (OR=2.88; 95%CI:1.50-4.34); (OR=1.7; 95%CI: 95%: 1.09-2.74) and being young were associated with high levels of stress, p<0.05. The TC practice during the pandemic promoted lower level of perceived stress, state anxiety and greater self-perception of happiness, showing the importance of TC practice and maintaining the frequency of physical activity during the pandemic.

Natural dentifrices: how can prolonged toothbrushing influence enamel color and surface roughness?

2023-12-12T08:45:22-03:00

This study evaluated the color change (ΔE00) and surface roughness (Ra) of enamel submitted to prolonged toothbrushing with natural non-fluoride dentifrices. Five groups of bovine teeth (n=10) were brushed with a control dentifrice: (C) Colgate Total 12 – Colgate; and four natural dentifrices: (R) Restore – Jeunesse; (OG) OnGuard – doTerra; (OC) OzonCare – Philozon; and (N) Natural activated charcoal – Suavetex. Mechanical toothbrushing simulated one month (T1), six months (T2), one year (T3), and two years (T4) of product use. ΔE00 and Ra were measured before and after toothbrushing. Two-way repeated measures ANOVA and Tukey's test were performed. Dentifrice (p<0.001), toothbrushing time (p=0.004), and their interaction (p=0.031) influenced color change (ΔE00). Natural dentifrices promoted similar color change and Ra to the traditional product. After six months of simulated toothbrushing, OG promoted more color changes than N and R. Only OG and R reached ΔE00 above the acceptability threshold. OC and N dentifrices generated Ra above the limit of clinical roughness from T2. Toothbrushing with natural toothpaste for up to two years causes similar enamel color changes and roughness to the traditional product.

Effects of dental tissue substructure and size on fracture strengths of lithium disilicate and zirconia ceramics

2023-08-26T19:32:03-03:00

We aimed to assess the effects of standard resin preparation models with five different thicknesses of occlusal surface on the fracture strengths of zirconia (ZrO2) and lithium disilicate glass ceramics. The specimens of 10 first maxillary molars collected between January 2019 and January 2020 were selected. Standard mathematical models were formed after scanning the resin matrices using software. The full crowns with five different thicknesses of occlusal surface were established, among which the molar specimens prepared by ZrO2 glass ceramic composites alone were assigned into ZrO2 group (n=5, 40 specimens) while those prepared using ZrO2-lithium disilicate glass ceramic composites were allocated into ZTCLDC group (n=5, 40 specimens). When the thickness of glass-ceramic full crowns was 0.5, 0.8, 1.0, 1.2 and 1.5 mm, the fracture load of the specimens in ZTCLDC group was not significantly different from that in ZrO2 group, and there was no significant difference in the three-point flexural strength between ZTCLDC group and ZrO2 group (P>0.05). The fracture toughness was not significantly different between the two groups in the case of the thickness of glass-ceramic full crown at 0.5, 0.8, 1.0, 1.2 and 1.5 mm (P>0.05). The thickness was positively correlated with fracture load, three-point flexural strength and fracture toughness (P<0.05). The fracture strength of lithium disilicate and ZrO₂ ceramics is directly proportional to the thickness of ZrO₂ and ZTCLDC crowns.

A multicenter and epidemiological study of patterns and treatment strategies for mandibular condyle fractures

2023-05-31T17:41:38-03:00

To evaluate retrospectively the epidemiological characteristics of the prevalence, type and treatment modalities of the condylar mandibular fractures. Data of all patients who underwent surgical or nonsurgical management for condyle mandibular fracture were collected. The study was conducted to assess the trauma etiology, age group, gender, treatment method, anatomic distribution of injury and complications by reviewing patients’ records. There were 139 patients with 171 mandibular condylar fractures. Among these patients, 85% were men, with an overall male-to-female ratio of 5.6:1. The highest occurrence of trauma was in the 21-30 years age group. The most frequently observed etiology was motorcycle traffic accidents and the subcondylar region was the most common location with 109 fractures. There was statistically significant difference between treatments (p < 0,001). In the nonsurgical treatment group, the prevalence of complications was observed compared to the surgical group (p < 0.001). The predominant complications were: mouth opening limitation, mandibular deviation, malocclusion, temporomandibular disorder, paresthesia and facial paralysis. Considering the limitations of this study, subcondylar fractures were the most frequent, especially in men aged 21-30 years, and the surgical treatment showed the fewest complications.

Influence of game therapy on urinary incontinence patients: a systematic review

2023-10-03T05:25:54-03:00

This study performed a systematic review of game therapy effects on urinary incontinence patients. Eleven databases (PubMed, SCOPUS, SciELO, LILACS, Web of Science, EMBASE, Cochrane library, LIVIVO, OpenGrey, OpenThesis, and OATD) were used as research sources. The search was conducted in January 2021 with the following keywords: urinary incontinence, urinary stress incontinence, pelvic floor muscle, pelvic floor training, virtual reality, game therapy, virtual reality exposure, and virtual reality therapy. The review included only clinical studies using game therapy for treating urinary incontinence in women without restrictions on language, year, and publication status. Only three studies fulfilled the eligibility criteria. The extracted data comprised sample characteristics (the number of patients in each study and average age) and treatment characteristics (game type, associated therapies, treatment duration, evaluated objectives, and result-measuring methods). The methodological quality of the articles showed a low risk of bias. One-hour pad test values decreased in all studies, and ICIQ-SF scores reduced in two articles. One study reported treatment adherence (92%), and another showed neuropsychological index improvements. The reduction of urinary symptoms was similar between the group associating game therapy with treatment and the group using only conventional pelvic floor muscle training. Game therapy, as a resource of pelvic floor muscle training, seems beneficial to urinary incontinence, including decreased urinary symptoms, lower one-hour pad test scores, and neuropsychological index improvements.

Synthesis of (-) - 6,6'-dinitrohinokinin from hinokinin natural product and in silico and in vitro trypanocidal activity assessment

2023-08-14T16:21:14-03:00

Chagas disease is a public health problem affecting approximately seven million people worldwide. Thus, there is a need to discover drugs for the adequate treatment of this disease because currently available drugs have serious side effects. Therefore, this study aimed to evaluate the in vitro trypanocidal activity of (-)-6,6'-dinitrohinokinin, obtained from the partial synthesis of (-)-hinokinin, on the trypomastigotes and amastigotes forms. For the trypomastigote assay, blood was collected from mice infected with Trypanosoma cruzi through cardiac puncture at the parasitemic peak. The results show that (-)-6,6'-dinitrohinokinin was effective against the trypomastigote forms, presenting an IC₅₀ of 19.83 µM and lysis percentage values of 78.4% and 69.4% at concentrations of 200 and 100 µM, respectively. Molecular docking calculations indicate that (-)-6,6'-dinitrohinokinin favorably interacts with the amino acids present in the active site of the protein trypanothione reductase, a typical target for anti-trypanosomal drug development. According to the results, the (-)-6,6'-dinitrohinokinin showed more significant trypanocidal activity with IC₅₀of 1.83 µM than benzonidazole positive control with IC₅₀of 53.2 µM, showing to be a prototype molecule promising for the development of a new antiparasitic drug.

Study of neutrophil/lymphocyte and platelet/lymphocyte ratios in type 2 diabetes mellitus

2023-08-10T11:52:10-03:00

The neutrophil-to-lymphocyte ratio (NLR) and platelet-to-lymphocyte ratio (PLR) are potential inflammatory biomarkers for prognostic analysis in several diseases, such as type 2 diabetes mellitus (T2DM). This study analyzed NLR and PLR as possible T2DM biomarkers. A cross-sectional study was conducted with a secondary database that included laboratory test results from two healthcare units in Goiânia, GO, Brazil, between 2015 and 2016. The participants were normoglycemic (NG), prediabetic (preDM), and had controlled DM (CDM) and uncontrolled DM (UDM). NLR and PLR were compared between study groups according to sex and age group. NLR was higher in group UDM than preDM (1.88 vs. 1.62, p <0.05) and lower in group preDM than NG (1.62 vs. 1.80, p <0.05). NG women aged ≥65 years were six times more likely to present PLR ≤91.83 than preDM ones (OR: 6.34; CI: 1.98 - 20.55; sensitivity = 41.5%, PPV = 84.6, and LR + = 4.15). NG men aged <65 years were almost four times more likely to present PLR ≤120 (OR: 3.64; CI: 1.21 - 10.89; sensitivity = 80%, PPV = 37.7, and LR + 1.53) than preDM ones. NLR and PLR increases are directly proportional to DM severity, except for group preDM compared to NG for NLR values. These biomarkers can help estimate T2DM prognosis.

The incidence of unfilled second mesiobuccal canals and periapical lesions in maxillary molars by cone-beam computed tomography E-VOL DX software

2023-12-18T19:19:52-03:00

This study evaluated the incidence of unfilled second mesiobuccal (MB2) canals and periapical lesions in first maxillary molars using CBCT e-Vol DX software. Hence, 326 CBCT images of first maxillary molars of patients undergoing endodontic treatment were selected. The e-Vol DX software performed a retrospective review of these images, recording the presence or absence of MB2 canals, filling, and periapical lesions in first maxillary molars. Specific statistical analysis was performed at a 5% significance level. Unfilled MB2 canals were highly frequent in first maxillary molars and significantly associated with periapical lesions in the mesiobuccal root of these teeth (p<0.05). The CBCT e-Vol DX software effectively detected MB2 canals, and unfilled canals may be associated with periapical lesions.

Morphometry of the infraorbital foramen in dentate and edentulous adult humans and its clinical relevance

2023-08-14T10:27:33-03:00

The infraorbital foramen (IOF) serves as an important anatomical reference for infraorbital nerve block during different procedures. Its clinical and surgical relevance, associated with conflicting results in its anatomy description justifies the present study. Thus, this work aims to describe the IOF, qualitatively and quantitatively, and propose an accurate measurement to define its location. One hundred and seventy-four IOFs of dry skulls were analyzed and divided in dentate (DE) and edentulous (ED) groups. Distances were measured between the IOF and the margin of the alveolar arch of the maxilla (MAAM), the inferior orbital margin (IOM), the intermaxillary suture (IMS), the vertical plane of IOF. A new methodology was proposed to optimize the identification of IOF based on the IMS distance. Oval-shaped IOFs (n=81/174) with inferomedial orientation (n=104/174) and with a crest-shaped upper margin (n=163/174) constituted the norm for this sample. Distance between IOF and MAAM was 29.97 ± 4.09 mm, to IOM 7.27 ± 1.8 mm, and to IMS 35.09 ± 3.77 mm. ED individuals had IOF closer to MAAM (p=0.0124). The highest point of this methodology was the lack of statistical differences in the IMS distance in ED and DE specimens, making this method applicable for both. Therefore, to minimize iatrogenic injuries, clinicians and surgeons must consider the total or partial presence of teeth, the methodology established to find the IOF, its inferomedial orientation, and the prevalent crest on its superior margin.

Predictors of efficacy, tolerability and discontinuation of Transcranial Direct Current Stimulation (tDCS) for Mild Cognitive Impairment (MCI) and Alzheimer’s Disease (AD): a meta-analysis and meta-regression

2023-05-17T12:58:58-03:00

Numerous patients with mild cognitive impairment (MCI) or Alzheimer’s disease (AD) are refractory to pharmacological treatment, and non-invasive brain neurostimulation has been investigated as another possibility for improving cognition. The performed meta-analysis and meta-regression verified predictors of efficacy, tolerability, and discontinuation of transcranial direct current stimulation (tDCS) for treating MCI or AD. The analyzed studies used the Mini-Mental State Exam, Montreal Cognitive Assessment, or Alzheimer's Disease Assessment Scale - Cognitive Subscale scores as outcome measures. Databases (PubMed, Embase, and Web of Science - primary collection) were searched, resulting in 12 published randomized and controlled trials. The risk of bias assessment was based on Cochrane Review recommendations, considering study characteristics. Other evaluated outcomes were the number of adverse effects (tolerability) and dropouts. Overall and anodal tDCS improved cognition compared to the sham protocol. Group comparisons did not show statistically significant differences for adverse effects and dropouts. Session duration was a response predictor, as stimulations of up to 20 minutes for ten days or more improved the outcome achievement. The AD diagnosis covariate also affected efficacy. The findings should be interpreted carefully in clinical practice because the stimulation effect may vary among subjects.

A comprehensive study on nutrient content of raw and roasted nuts

2023-08-25T10:07:58-03:00

Nuts are highly valued for their properties and are consumed worldwide owing to their health benefits, particularly, for the prevention and treatment of diseases, as well as a source of essential macro- and micronutrients. This study aimed to evaluate the effects of roasting on the nutritional value of nuts. Three types of nuts, raw and roasted almonds, cashews, and walnuts were selected from three well-known companies in Saudi Arabia. To analyze and evaluate the stability of macro- and micronutrients in nuts, modern separation and quantification methods, including inductively coupled plasma-optical emission spectrometry (ICP-OSE), were used to analyze and evaluate the stability of nutrients and heavy metals in nuts that are widely distributed and consumed. The results of this study indicate that nuts have high nutritional value and an inverse roasting effect. Comparing the nutrient and metal contents of raw and roasted nuts provides useful information on the nutritional science of nuts consumed by well-known manufacturers in the Kingdom of Saudi Arabia. Further investigations of nuts are needed to draw firm conclusions regarding their association with the prevention of many different diseases.